FUNCTIONAL RECONSTRUCTION OF NEURONAL SYSTEMS

神经系统的功能重建

• 批准号: 3410423
• 负责人: SCOTT E FRASER
• 金额: $ 13.34万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3410423
• 关键词: calcium channel; computer simulation; computer system design /evaluation; mathematical model; membrane channels; membrane potentials; neurosciences; neurotransmitter receptor; neurotransmitters; second messengers

The overall aim of the proposed projects is to develop, refine, and make available to the neuroscientific community a body of computer software for rigorously based simulation of the behavior of neuronal systems. The programs embody a compartmental description of neuronal morphology and include such mechanisms as voltage-dependent ion channels, kinetics of calcium-ion binding by a number of substrates, mobilization and packaging of neurotransmitter in vesicles, calcium-triggered release of transmitter quanta into the synaptic cleft, receptors for neurotransmitter that produce time- and voltage-dependent conductance changes or a time-varying concentration of second messenger, and modulation of ion channels by calcium ion or by second messenger. Available manipulations include steady or time-varying current injection, deterministic or stochastic stimulation of axons, single- or dual- electrode voltage clamps, and intracellular injection of calcium ion. Among the variables that can be recorded and plotted are membrane potentials, individual ionic and synaptic currents, individual and summed conductances, calcium-ion concentrations, free and bound neurotransmitter, and receptor states. The programs will be interactive and will make extensive use of high-resolution color graphics. They will enable neurobiologists to organize data collection and will provide theoretical frameworks for testing hypotheses and comparing prediction with experiment. Use of such models enriches the information obtained from animal experiments and eventually will make possible more explicit and quantitative determination of therapeutic neuropharmacological regimes.

拟议项目的总体目标是开发、完善和 为神经科学界提供一系列 用于严格模拟行为的计算机软件 的神经系统。 这些程序体现了神经元的分区描述 形态并包括电压依赖性等机制 离子通道，钙离子结合的动力学 神经递质的底物、动员和包装 囊泡，钙触发的递质量子释放到 突触间隙，神经递质受体，产生时间- 和电压相关的电导变化或随时间变化 第二信使的浓度和离子的调制 通过钙离子或第二信使的通道。 可用的 操纵包括稳定或时变电流注入， 轴突的确定性或随机刺激，单或双 电极电压钳和细胞内钙注射 离子。 可以记录和绘制的变量包括 膜电位、个体离子电流和突触电流， 单独和总电导、钙离子浓度、 游离和结合的神经递质和受体状态。 这些计划将是互动的，并将广泛利用 高分辨率彩色图形。 它们将使神经生物学家能够 组织数据收集并提供理论框架 用于检验假设并将预测与实验进行比较。 使用此类模型丰富了从动物身上获得的信息 实验并最终将使更加明确和 治疗神经药理学的定量测定 政权。