NEURAL REGULATION OF THIRST

口渴的神经调节

• 批准号: 3406077
• 负责人: MASSAKO KADEKARO-KUTYNA
• 金额: $ 13.74万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3406077
• 关键词: acetylcholine; angiotensin /renin /aldosterone hypertension; angiotensin II; autoradiography; baroreceptors; brain metabolism; cerebral ventricles; denervation; homeostasis; laboratory rat; neural information processing; neurotransmitters; prosencephalon; serotonin; thirst; water drinking behavior

The objective of this study is to investigate the neural and neuroendocrine mechanisms triggered by increases in plasma osmolality. We will test the hypothesis that stimuli signalling reductions in intracellular volume to bring about body fluid homeostasis originate primarily in areas within the anteroventral third ventricle (AV3V) area and secondarily in areas surrounding the supraoptic nucleus. To accomplish these goals, we will employ the quantitative autoradiographic (14C)deoxyglucose technique. The specific aims of this study are: 1. To measure water intake and glucose utilization in the brain and hypothalamo-neurohypophysial system during intracarotid or intracerebroventricular infusion of hypertonic saline. This study will allow us to: 1) identify the body fluid homeostatic neural pathways involved in the osmoreceptor reflex; and 2) examine whether infusion of hypertonic saline by different routes activate the same neural substrates to bring about body fluid homeostasis. 2. To compare the effects of lesion of the AV3V area with that of sham-operated animals on glucose utilization in the pathways activated by different concentrations of intracarotid or intracerebroventricular infusions of hypertonic saline and relate them with the animal's water drinking behavior. This study will allow us to identify whether the afferent limb of the osmoreceptor reflex originates or crosses in the AV3V area and whether other mechanisms outside the AV3V area can be activated by a higher degree of plasma or cerebrospinal fluid osmolality. 3. To compare the effects of intravenous administration of hexamethonium, a nicotinic blocker agent, and phenoxybenzamine, an alpha-adrenergic blocker, on the basal activity and on the hyperosmotic-stimulated activity of the hypothalamo- neurohypophysial system and relate these findings with the animals, water drinking behavior. This study will allow us to understand how the cholinergic and adrenergic mechanisms are functionally related in the osmoreceptor reflex pathway.

这项研究的目的是研究神经和 血浆增加触发的神经内分泌机制 渗透压。 我们将测试刺激信号传导的假设 减少细胞内体积以产生体液 稳态主要源于前腹区域内的区域 第三脑室（AV3V）区域，其次在周围的区域 上核。 为了实现这些目标，我们将采用 定量放射自显影（14C）脱氧葡萄糖技术。 这项研究的具体目的是： 1。测量大脑中的饮水和葡萄糖利用 和下丘脑神经植物学系统期间或 脑室内输注高渗盐水。 这项研究 将允许我们：1）识别体液稳态神经 渗透受体反射涉及的途径； 2）检查 通过不同路线注入高渗盐水是否激活 相同的神经底物可引起体液稳态。 2。将AV3V区域病变的影响与 假动物在途径中的葡萄糖利用率上 通过不同浓度的核内或 室内室内输注高渗盐水并相关 他们以动物的喝水行为。 这项研究会 允许我们确定渗透受体的传入肢体是否 反射起源于AV3V区域以及其他是否存在 AV3V区域以外的机制可以通过较高的 血浆或脑脊液渗透压的程度。 3。比较静脉给药的影响 六甲基，烟碱阻滞剂和苯氧苯甲胺， α-肾上腺素能阻滞剂，基础活动和 下丘脑的过度刺激性活性 神经型物理系统，并将这些发现与动物相关联 喝水的行为。 这项研究将使我们能够理解 胆碱能和肾上腺素能的机制在功能上如何 相关在孔反射途径中相关。