NEURAL REGULATION OF THIRST

口渴的神经调节

• 批准号: 3406077
• 负责人: MASSAKO KADEKARO-KUTYNA
• 金额: $ 13.74万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3406077
• 关键词: acetylcholine; angiotensin /renin /aldosterone hypertension; angiotensin II; autoradiography; baroreceptors; brain metabolism; cerebral ventricles; denervation; homeostasis; laboratory rat; neural information processing; neurotransmitters; prosencephalon; serotonin; thirst; water drinking behavior

The objective of this study is to investigate the neural and neuroendocrine mechanisms triggered by increases in plasma osmolality. We will test the hypothesis that stimuli signalling reductions in intracellular volume to bring about body fluid homeostasis originate primarily in areas within the anteroventral third ventricle (AV3V) area and secondarily in areas surrounding the supraoptic nucleus. To accomplish these goals, we will employ the quantitative autoradiographic (14C)deoxyglucose technique. The specific aims of this study are: 1. To measure water intake and glucose utilization in the brain and hypothalamo-neurohypophysial system during intracarotid or intracerebroventricular infusion of hypertonic saline. This study will allow us to: 1) identify the body fluid homeostatic neural pathways involved in the osmoreceptor reflex; and 2) examine whether infusion of hypertonic saline by different routes activate the same neural substrates to bring about body fluid homeostasis. 2. To compare the effects of lesion of the AV3V area with that of sham-operated animals on glucose utilization in the pathways activated by different concentrations of intracarotid or intracerebroventricular infusions of hypertonic saline and relate them with the animal's water drinking behavior. This study will allow us to identify whether the afferent limb of the osmoreceptor reflex originates or crosses in the AV3V area and whether other mechanisms outside the AV3V area can be activated by a higher degree of plasma or cerebrospinal fluid osmolality. 3. To compare the effects of intravenous administration of hexamethonium, a nicotinic blocker agent, and phenoxybenzamine, an alpha-adrenergic blocker, on the basal activity and on the hyperosmotic-stimulated activity of the hypothalamo- neurohypophysial system and relate these findings with the animals, water drinking behavior. This study will allow us to understand how the cholinergic and adrenergic mechanisms are functionally related in the osmoreceptor reflex pathway.

本研究的目的是研究神经和 血浆增加触发的神经内分泌机制 渗透压。 我们将检验刺激信号传导的假设 细胞内容量减少，导致体液减少 体内平衡主要起源于前腹侧区域 第三脑室 (AV3V) 区域，其次是周围区域 视上核。 为了实现这些目标，我们将采用 定量放射自显影（14C）脱氧葡萄糖技术。 本研究的具体目的是： 1. 测量大脑的水摄入量和葡萄糖利用率 和下丘脑神经垂体系统在颈内动脉或 脑室内输注高渗盐水。 这项研究 将使我们能够：1）识别体液稳态神经 参与渗透压感受器反射的途径； 2）检查 不同途径输注高渗盐水是否激活 相同的神经基质带来体液稳态。 2. 比较 AV3V 区病变与 AV3V 区病变的影响 假手术动物对葡萄糖利用途径的影响 不同浓度的颈内动脉或 脑室内输注高渗盐水及其相关 他们与动物的饮水行为。 这项研究将 让我们能够识别渗透压感受器的传入肢是否 反射起源或交叉于 AV3V 区，以及是否有其他反射 AV3V 区域之外的机制可以由更高的激活 血浆或脑脊液渗透压的程度。 3. 静脉给药效果比较 六甲铵（一种烟碱阻滞剂）和苯氧苯胺（一种烟碱阻滞剂） α-肾上腺素能阻滞剂，对基础活性和 高渗刺激的下丘脑活动 神经垂体系统并将这些发现与动物联系起来， 饮水行为。 这项研究将使我们了解 胆碱能和肾上腺素能机制如何发挥作用 与渗透压感受器反射途径有关。