The development of circularly polarised luminescence microscopy and responsive CPL probes

圆偏振发光显微镜和响应性 CPL 探针的发展

• 批准号: EP/I010319/1
• 负责人: David Parker
• 金额: $ 81.73万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FI010319%2F1
• 关键词: development; circularly; polarised; luminescence; microscopy

Polarised light is made up by adding together right handed and left handed circularly polarised components. Certain metal ions can emit light when they are raised into a high energy state. If the environment around that metal ion is chiral, i.e. left or right handed, then the emitted light can be enriched in the left or right handed component. This phenomenon is called circularly polarised luminescence. It is most well-defined in the emission of red or green light from chiral europium and terbium complexes, and has been observed for several complexes of these metal ions. When a chiral metal complex is located in the presence of other species that are right or left-handed, the emitted light may change its circular polarisation and this change can be tracked with time using a spectrometer that records these changes. Thus, the local chiral environement of the 'probe' metal complex is reported by changes to the circular polarisation of its emission.In this project, we seek to track such changes while observing the spatial localisation of the light-emitting metal complex using a microscope. We will build for the first time, a microscope that is able to monitor changes in the circular polarisation of emitted light from complexes of the emissive europium and terbium metal ions. In parallel, we shall make a series of left and right handed metal complexes that are not only emissive and resist degradation, but also can bind to important bioactive species that are themselves chiral, such as the protein, serum albumin, or the common chiral molecule, vitamin C. We have already shown that related emissive metal complexes can be engineered to be cell-permeable and can be designed to reside preferentially in certain compartments within a living cell. Thus, we shall pioneer the tracking or mapping of these bioactive chiral probes inside a living cell, using the new microscope to monitor their unique optical signal that reports on their local surroundings.

偏振光是通过将右手和左手圆偏振分量相加而成的。某些金属离子在进入高能态时可以发光。如果该金属离子周围的环境是手性的，即左手或右手，则发射的光可以在左手或右手成分中富集。这种现象称为圆偏振发光。它在手性铕和铽配合物发出的红光或绿光中最为明确，并且已经在这些金属离子的几种配合物中观察到。当手性金属配合物存在于其他右旋或左旋物质存在时，发射的光可能会改变其圆偏振，并且可以使用记录这些变化的光谱仪随时间跟踪这种变化。因此，“探针”金属配合物的局部手性环境是通过其发射的圆偏振的变化来报告的。在这个项目中，我们试图在使用显微镜观察发光金属配合物的空间定位的同时跟踪这些变化。 。我们将首次建造一台能够监测铕和铽金属离子复合物发射光圆偏振变化的显微镜。与此同时，我们将制造一系列左手和右手金属配合物，它们不仅具有发射性和抗降解性，而且还可以与本身手性的重要生物活性物质结合，例如蛋白质、血清白蛋白或常见的手性分子我们已经证明，相关的发射金属络合物可以被设计为可渗透细胞，并且可以被设计为优先驻留在活细胞内的某些隔室中。因此，我们将率先在活细胞内跟踪或绘制这些生物活性手性探针，使用新的显微镜监测其独特的光学信号，报告其局部环境。

项目成果

A chiral probe for the acute phase proteins alpha-1-acid glycoprotein and alpha-1-antitrypsin based on europium luminescence.

基于铕发光的急性期蛋白α-1-酸性糖蛋白和α-1-抗胰蛋白酶的手性探针。

• DOI: 10.1039/c2dt30143a
• 发表时间: 2012-10-10
• 期刊: Dalton transactions
• 影响因子: 4
• 作者: Rachel Carr;L. di Bari;Samuele Lo Piano;D. Parker;R. Peacock;John M. S;erson;erson
• 通讯作者: erson

Targeted pH switched europium complexes monitoring receptor internalisation in living cells.

靶向 pH 值切换的铕络合物监测活细胞中受体的内化。

• DOI: http://dx.10.1039/d1cc01029h
• 发表时间: 2021
• 期刊: Chemical communications (Cambridge, England)
• 作者: Fradgley JD

Utility of tris(4-bromopyridyl) europium complexes as versatile intermediates in the divergent synthesis of emissive chiral probes.

三（4-溴吡啶基）铕配合物作为发射手性探针的发散合成中的多功能中间体的用途。

• DOI: http://dx.10.1039/c4dt00253a
• 发表时间: 2014
• 期刊: 2003)
• 作者: Butler SJ

Lanthanide complexes as chiral probes exploiting circularly polarized luminescence.

稀土配合物作为利用圆偏振发光的手性探针。

• DOI: 10.1039/c2cs35242g
• 发表时间: 2012-11-05
• 期刊: Chemical Society reviews
• 影响因子: 46.2
• 作者: Rachel Carr;N. H. Evans;D. Parker
• 通讯作者: D. Parker

Chemistry-A European Journal

化学-欧洲期刊

• 期刊: Chemistry a European Journal
• 作者: David Parker (Author)