OPTICAL RANGING OF HB/MB IN TISSUES

组织中 HB/MB 的光学测距

• 批准号: 3362901
• 负责人: BRITTON CHANCE
• 金额: $ 19.63万
• 依托单位: UNIVERSITY CITY SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3362901
• 关键词: biomedical equipment development; blood vessel disorder; clinical biomedical equipment; cryopreservation; disease /disorder model; dogs; exercise; hemoglobin; human subject; hypoxia; imaging /visualization /scanning; infarct; interferometry; laser spectrometry; limbs; magnetic resonance imaging; myocardial ischemia /hypoxia; myocardium; myoglobin; phosphorus; respiratory oxygenation; spectrometry; striated muscles; syndrome; tissue /organ preservation

This research program calls for an intensive development and quantitation of a new technology for noninvasive measurements of hemoglobin/myoglobin deoxygenation in skeletal and cardiac muscle. A continuous light method (CWS) is base upon the dual wavelength spectrophotometry principle and can be attached directly to the limb of the exercising animal or human subject. This instrument is a "trend indicator" and is calibrated in relation to cuff ischemia and hyperemia where possible. The optical path is unknown and only relative responses are obtained with human subjects with peripheral vascular disease or congestive heart failure. The results can be closely correlated with those obtained by phosphorus magnetic resonance spectroscopy. In order to improve quantitation by direct measurement of the optical path, time resolved spectroscopy (TRS) involving picosecond or phase modulation spectroscopy (PMS) technologies afford a quantitation of concentration changes for a single wavelength device and direct measurements of concentrations and their changes for a dual and multi- wavelength animal models in normoxic and ischemic limbs. Furthermore, the separate contributions of Hb/Mb will be evaluated by 1) chemical modification of myoglobin, 2) highly resolved laser spectroscopy, 3) synchronization of hemoglobin signals with the arteriolar pulse, and 4) freeze trapping and redox scanning at low temperatures. The imaging potential of TRS will be studied in models, in normal animals and in limbs with an induced compartment syndrome and in model infarcts of the myocardium using single channel CWS and PMS. Upgrading PMS and TRS to multichannel spectroscopy of these models will provide the data sets which can be analyzed by appropriate algorithms by which the imaging potential can be evaluated with the ultimate goal providing a 3-D motion picture of Hb/Mb changes over the contraction cycle. The hypoxic tissue volume of each model will be evaluated by freeze trapping and 3-D redox scanning under other support. Observations through the chest wall will be attempted where synchrony with systole-distole may afford identification of myocardial tissue volumes, with an end in view of providing methods applicable to human subjects. Thus safe, economical and efficient evaluation of muscle hypoxia/ischemia may become generally available to general and speciality medical evaluations without the use of large magnets or radioactive materials.

该研究计划要求进行密集的开发和定量 用于血红蛋白/肌红蛋白无创测量的新技术 骨骼肌和心肌的脱氧。 连续的光方法 （CWS）基于双波长分光光度法原理，并且可以 直接固定在运动动物或人类受试者的肢体上。 该仪器是“趋势指标”，并且经过校准 袖口缺血和充血。 光学路径未知 并且仅通过人类受试者获得相对反应 周围血管疾病或充血性心力衰竭。 结果可以 与通过磷磁共振获得的密切相关 光谱法。 为了通过直接测量来改善定量 涉及picsecond或 相位调制光谱（PMS）技术提供了定量 单个波长设备的浓度变化并直接 浓度及其变化的测量 在常氧和缺血性四肢中的波长动物模型。 此外， HB/MB的单独贡献将通过1）化学评估 肌红蛋白的修饰，2）高度分辨的激光光谱，3） 血红蛋白信号与小动脉脉冲的同步，4） 在低温下冻结捕获和氧化还原扫描。 成像 TR的潜力将在模型，正常动物和四肢中研究 具有诱导室综合征和模型梗死 心肌使用单个通道CWS和PMS。 将PM和TRS升级到 这些模型的多通道光谱将提供数据集 可以通过适当的算法来分析成像电位的算法 可以用最终目标评估3-D电影 HB/MB在收缩周期内发生变化。 低氧组织体积 每个模型将通过冻结和3-D氧化还原扫描来评估 在其他支持下。 将尝试通过胸壁观察 与基质脱轴同步的地方可能可以识别 心肌组织量，鉴于提供方法 适用于人类受试者。 因此安全，经济和高效 肌肉缺氧/缺血的评估通常可以用于 一般和专业的医学评估，无需使用大磁铁 或放射性材料。