Microneedle-mediated enhanced Raman therapeutic drug monitoring

微针介导的增强拉曼治疗药物监测

• 批准号: EP/H021647/1
• 负责人: Ryan Donnelly
• 金额: $ 41.72万
• 依托单位: Queen's University Belfast
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2010
• 资助国家: 英国
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FH021647%2F1
• 关键词: Microneedle; mediated; enhanced; Raman; therapeutic

Over the past 3 decades there has been a dramatic increase in the number of people infected with human immunodeficiency virus and Mycobacterium tuberculosis, such that approximately 40 million are currently infected by HIV and 14 million are infected with tuberculosis. Strict compliance with prescribed drug treatment regimens is required for management of these diseases in individuals and control of their spread to others. Monitoring of compliance using conventional methods is problematic, particularly in the Developing World, where lack of resources and improper use of needles causes significant problems for society. Advances in medical treatment mean more premature neonates now survive. Due to their prematurity and the associated complications, these patients are frequently treated with multiple drugs. Direct blood sampling can, however, cause severe bruising or scarring and such patients also have very limited blood volumes, which prevents frequent sampling. One in five fatal car accidents in the UK are currently due to drivers driving under the use of illegal or prescription drugs. No appropriate roadside test currently exists. When considering these factors, in addition to the time-consuming nature and expense of routine therapeutic drug monitoring in hospitals, it is obvious that development of novel non- or minimally-invasive technologies that permit rapid and frequent ambulatory monitoring without drawing of blood is extremely important. In this project, we will investigate novel technology based on tiny needles that puncture the outer layer of skin without causing any pain or bleeding - the sensation is said to feel like a cat's tongue or sharkskin. These needles will then swell, turning into a jelly-like material that keeps the holes open and allow collection of skin fluid. As the drug content in the fluid in people's skin is very similar to that in their blood, we can use our microneedles, in combination with a sophisticated measurement technique, known as Surface-enhanced Raman Spectroscopy, to accurately monitor blood levels of drugs.Once developed, the technology described here will allow frequent routine monitoring of patients. Accordingly, drug- and exogenous substance-associated adverse events and complications arising from blood sampling will be prevented, to the benefit of patients Worldwide. In the UK, the NHS will benefit from reduced costs due to shorter hospital stays and reduced occurrence of inappropriate dosing. Ultimately, health-related-quality-of-life will be enhanced through improved disease control, rapid detection of dangerously high or low levels, facile monitoring of adherence to prescribed regimens (eg treatment of tuberculosis in the Developing World) and detection of illicit substances in addicts or vehicle drivers. Preterm neonates are likely to derive great benefit from the marked increase in monitoring frequency permitted, as are elderly patients being treated with multiple drugs. Ultimately, commercialisation of the technology will be the primary route by which UK industry, the NHS and patients will derive benefits. In order to attract potential industrial partners, it is vitally important to demonstrate proof of concept for this technology, which is the over-arching aim of the present proposal.

过去30年来，人类免疫缺陷病毒和结核分枝杆菌感染人数急剧增加，目前约有4000万人感染艾滋病毒，1400万人感染结核病。需要严格遵守规定的药物治疗方案来管理个人的这些疾病并控制其传播给他人。使用传统方法监测合规性是有问题的，特别是在发展中国家，资源匮乏和针头使用不当给社会带来了严重问题。医疗技术的进步意味着现在有更多的早产儿能够存活。由于早产和相关并发症，这些患者经常接受多种药物治疗。然而，直接采血可能会导致严重的瘀伤或疤痕，而且此类患者的血量也非常有限，这妨碍了频繁的采血。目前，英国五分之一的致命车祸是由于司机使用非法药物或处方药驾驶造成的。目前不存在适当的路边测试。考虑到这些因素，除了医院常规治疗药物监测的耗时性和费用外，显然，开发能够在不抽血的情况下快速、频繁地进行动态监测的新型非侵入性或微创技术是极其重要的。重要的。在这个项目中，我们将研究基于小针的新技术，这种技术可以刺穿皮肤外层，不会引起任何疼痛或流血——据说这种感觉就像猫的舌头或鲨鱼皮一样。然后这些针会膨胀，变成果冻状材料，使孔保持打开状态并允许收集皮肤液体。由于人皮肤液体中的药物含量与血液中的药物含量非常相似，因此我们可以使用我们的微针，结合复杂的测量技术（称为表面增强拉曼光谱）来准确监测血液中的药物浓度。随着技术的发展，这里描述的技术将允许对患者进行频繁的常规监测。因此，将预防因血液采样而引起的与药物和外源性物质相关的不良事件和并发症，从而造福于全世界的患者。在英国，由于住院时间缩短和不适当剂量的发生减少，国民医疗服务体系（NHS）将受益于成本降低。最终，通过改善疾病控制、快速检测危险的高或低水平、轻松监测对规定治疗方案的遵守情况（例如发展中国家的结核病治疗）以及检测非法物质，将提高与健康相关的生活质量吸毒者或车辆司机。早产儿可能会从允许监测频率的显着增加中获得巨大益处，接受多种药物治疗的老年患者也是如此。最终，该技术的商业化将成为英国工业界、NHS 和患​​者受益的主要途径。为了吸引潜在的工业合作伙伴，展示该技术的概念验证至关重要，这是本提案的首要目标。

项目成果

Hydrogel-forming microneedle arrays: Potential for use in minimally-invasive lithium monitoring.

水凝胶形成的微针阵列：用于微创锂监测的潜力。

• DOI: 10.1016/j.ejpb.2016.03.009
• 发表时间: 2016-05-01
• 期刊: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
• 作者: E. Eltayib;A. Brady;E. Caffarel;P. González;Ahlam Zaid Alkilani;H. McCarthy;J. McElnay;R. Donnelly
• 通讯作者: R. Donnelly

Swellable polymer films containing Au nanoparticles for point-of-care therapeutic drug monitoring using surface-enhanced Raman spectroscopy.

含有金纳米粒子的可膨胀聚合物薄膜，用于使用表面增强拉曼光谱进行即时治疗药物监测。

• DOI: 10.1016/j.aca.2016.01.023
• 发表时间: 2016-03-17
• 期刊: Analytica chimica acta
• 影响因子: 6.2
• 作者: Wendy W. Y. Lee;C. McCoy;R. Donnelly;S. Bell
• 通讯作者: S. Bell

Transdermal on-demand drug delivery based on an iontophoretic hollow microneedle array system.

基于离子电渗空心微针阵列系统的透皮按需给药。

• DOI: 10.1039/d3lc00160a
• 发表时间: 2023-04-19
• 期刊: Lab on a Chip
• 影响因子: 6.1
• 作者: Usanee Detamornrat;Marc Parrilla;Juan Domínguez-Robles;Qonita Kurnia Anjani;Eneko Larrañeta;Karolien De Wael;Ryan F. Donnelly
• 通讯作者: Ryan F. Donnelly

Microarray patches: potentially useful delivery systems for long-acting nanosuspensions.

微阵列贴片：长效纳米混悬液的潜在有用的输送系统。

• DOI: http://dx.10.1016/j.drudis.2017.10.013
• 发表时间: 2018
• 期刊: Drug discovery today
• 影响因子: 7.4
• 作者: Donnelly RF

Hydrogel-Forming Microneedle Arrays for Enhanced Transdermal Drug Delivery.

用于增强透皮药物输送的水凝胶形成微针阵列。

• DOI: http://dx.10.1002/adfm.201200864
• 发表时间: 2012
• 期刊: Advanced functional materials
• 影响因子: 19
• 作者: Donnelly RF