Cross talk between DNA replication and LPS biosynthesis during cell growth

细胞生长过程中 DNA 复制和 LPS 生物合成之间的串扰

• 批准号: BB/Y001265/1
• 负责人: Manuel Banzhaf
• 金额: $ 55.71万
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FY001265%2F1
• 关键词: Cross; talk; between; DNA; replication

Bacteria are everywhere and their presence affects our daily life. They can be our friends, by helping us to digest inaccessible nutrients in our guts, producing the oxygen we breathe, degrading toxic materials or fermenting the foods we love. They can also be our foes by making us sick or spoiling our food. Accepting that we will indefinitely share our planet with them, we think it best to understand in great detail how bacteria grow. This information will help us to get better at selectively destroying the bad bacteria around us, but may also enable us to engineer more efficient bacterial strains for biotechnological or medical applications. The bacterial life cycle consists of several steps. First, cells need to grow in biomass, enlarge its shell, the bacterial envelope and copy its DNA. With all of this completed, the bacterial cells start a process we call cell division. During this process, the bacterium divides in half creating two individual cells that have all it takes to restart their growth cycle for the next generation. These steps happen at the same time during growth, therefore they must be coordinated. However, exactly how bacterial cells coordinate DNA replication and growth of their cell envelopes is not yet known. Studying this question, our laboratory found that one of the master regulators of DNA replication, DiaA, might be the answer. We have shown that DiaA is genetically linked to envelope biogenesis and recognises a metabolite in envelope synthesis. With this proposal we want to better understand how DiaA communicates with envelope biogenesis to coordinate the two processes. We think a detailed understanding of this process is important because knowing this could help us to make bacteria grow better if its desirable, or reduce their growth where it is not.

细菌无处不在，它们的存在影响着我们的日常生活。它们可以成为我们的朋友，帮助我们消化肠道中难以获取的营养物质，产生我们呼吸的氧气，降解有毒物质或发酵我们喜爱的食物。它们也可能成为我们的敌人，让我们生病或破坏我们的食物。考虑到我们将无限期地与它们共享我们的星球，我们认为最好详细了解细菌如何生长。这些信息将帮助我们更好地选择性地消灭我们周围的坏细菌，但也可能使我们能够为生物技术或医学应用设计更有效的细菌菌株。细菌的生命周期由几个步骤组成。首先，细胞需要在生物质中生长，扩大其外壳、细菌包膜并复制其 DNA。所有这些完成后，细菌细胞开始一个我们称为细胞分裂的过程。在此过程中，细菌分裂成两半，产生两个单独的细胞，这些细胞拥有重新启动下一代生长周期所需的一切。这些步骤在生长过程中同时发生，因此它们必须协调一致。然而，细菌细胞究竟如何协调 DNA 复制和细胞包膜的生长尚不清楚。通过研究这个问题，我们的实验室发现 DNA 复制的主要调节因子之一 DiaA 可能就是答案。我们已经证明 DiaA 与包膜生物发生有遗传联系，并识别包膜合成中的代谢物。通过这个提议，我们希望更好地了解 DiaA 如何与包膜生物发生进行通信以协调这两个过程。我们认为详细了解这个过程很重要，因为了解这一过程可以帮助我们在需要时使细菌生长得更好，或者在不需要时减少细菌的生长。