A New Platform for Real Time Analysis of Metabolic Processes in Live Cells

实时分析活细胞代谢过程的新平台

• 批准号: BB/X019098/1
• 负责人: Tessa Moses
• 金额: $ 30.04万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FX019098%2F1
• 关键词: New; Platform; Real; Time; Analysis

We request an Agilent Seahorse XF Pro analyser interfaced with Cytation 1 imager and software for high throughput real-time analysis of metabolic processes in live cells.Metabolic phenotyping is the term given to the comprehensive analysis of biological samples, such as body fluids (eg urine, blood, saliva), tissues (eg muscle, epithelial, nervous), or cells (eg bacteria, yeast, mammalian) to understand physiological (healthy) or pathological (disease) states. Analytical technologies for metabolic phenotyping of living cells in real-time are essential to fully understand biological processes in their near-to-native state. The routine analysis of cellular mechanisms has the potential to improve our understanding of cell growth and implications of cell aging, impact of environmental changes on cellular health, initiation of disease in healthy cells, effect of drugs or treatments on diseased cells, and impact of interventions on cellular homeostasis. Majority of our existing understanding of metabolic phenotypes involve cell disruption (breaking cells open) to access and analyse its metabolome, or the analysis of the cellular environment to deduce internal cellular bioprocesses. Neither of these approaches accurately represent the native state of metabolism in living cells. It is therefore essential to probe cellular metabolic processes in live cells to better understand biology, diseases, aging, as well as to improve the use of cells in engineering organisms for valuable commodities (such as anti-cancer drugs, vaccines, flavours and fragrances, plastic alternatives, etc).The Agilent Seahorse XF Pro analyser is an instrument that can measure vital cellular processes in live cells, without using disruptive (cell lysis) techniques. The instrument preserves cell integrity during analysis and allows repeat measurements to be taken on the same cells over time, providing an unprecedented depth in metabolic phenotyping of live cells. Additionally, the instrument is easy-to-use, has high throughput, is compatible with a wide variety of cell types, requires minimal cleaning and calibration, and is user-friendly with an intuitive software platform, making it an ideal instrument for multi-user environments, such as the EdinOmics facility. By adding this capability to EdinOmics existing portfolio of analytical technologies, we will be able to provide world leading researchers at the University of Edinburgh with a high throughput metabolic phenotyping technology to improve research productivity, minimise experiment costs, reduce time to results and speed up technology translation to market.

We request an Agilent Seahorse XF Pro analyser interfaced with Cytation 1 imager and software for high throughput real-time analysis of metabolic processes in live cells.Metabolic phenotyping is the term given to the comprehensive analysis of biological samples, such as body fluids (eg urine, blood, saliva), tissues (eg muscle, epithelial, nervous), or cells (eg bacteria, yeast, mammalian)了解生理（健康）或病理（疾病）状态。实时对活细胞代谢表型的分析技术对于完全了解其近乎本地状态的生物学过程至关重要。细胞机制的常规分析有可能提高我们对细胞生长的理解和细胞衰老的影响，环境变化对细胞健康的影响，健康细胞中疾病的启动，药物或治疗对患病细胞的影响以及干预对细胞稳态的影响。我们对代谢表型的现有理解的大多数涉及细胞破坏（打开细胞开放）以访问和分析其代谢组，或分析细胞环境以推断内部细胞生物处理。这些方法都不能准确代表活细胞中代谢的本地状态。因此，必须在活细胞中探测细胞中的细胞代谢过程，以更好地了解生物学，疾病，衰老，以及改善细胞在工程生物中的使用来用于有价值的商品（例如抗癌药物，疫苗，风味和香气，香料和香水，塑料替代品等）。技术。该仪器在分析过程中保留了细胞完整性，并允许随着时间的推移对同一细胞进行重复测量，从而为活细胞的代谢表型提供了前所未有的深度。此外，该仪器是易于使用的，具有高通量，与多种细胞类型兼容，需要最少的清洁和校准，并且具有直觉的软件平台对用户友好，使其成为多用户环境的理想工具，例如Edinomics设施。通过将这种能力添加到现有分析技术的现有投资组合中，我们将能够为爱丁堡大学的世界主要研究人员提供高吞吐剂的代谢表型技术，以提高研究生产力，最大程度地降低实验成本，减少时间的成本，并将结果降低到结果并加快技术转换为市场。