SYNTHETIC ANALOGS FOR METAL-CYSTEINE CONTAINING PROTEINS

含有金属半胱氨酸的蛋白质的合成类似物

基本信息

批准号：
3290027
负责人：
MICHELLE M. MILLAR
金额：
$ 7.42万
依托单位：
STATE UNIVERSITY OF NEW YORK AT ALBANY
依托单位国家：
美国
项目类别：
财政年份：
1985
资助国家：
美国
起止时间：
1985-07-01 至 1986-07-31
项目状态：
已结题

项目摘要

Our research objective is to synthesize and fully characterize new types of metal-thiolate complexes for the purpose of obtaining fundamental information about the structural, spectroscopic and reactivity properties of proteins containing metal-cysteine interactions. The metal-cysteine ligation mode occurs in a wide range of metalloproteins. We intend to prepare synthetic analogs for a number of metal-thiolate containing proteins; in particular those proteins which contain: iron (the iron-sulfur proteins), copper (blue copper and cytochrome c oxidase), zinc (alcohol dehydrogenase, aspartate transcarbamylase and metallothionein), cadmium (metallothionein) and molybdenum (xanthine oxidase and dehydrogenase, aldehyde oxidase, sulfite oxidase and nitrate reductase). Moreover, a number of metals (including cobalt, nickle, cadmium, iron and copper) have been incorporated into these proteins as spectroscopic probes. Chemico-physical studies of these model compounds will be used to achieve a fundamental understanding of the relationship between spectroscopic properties and the structural and electronic features of the metal center. In addition, the spectroscopic parameters determined from a variety of metal-thiolate model compounds should be useful to biochemists in their efforts to identify different types of metal-cysteine coordination modes.

我们的研究目标是合成并充分表征新型金属硫醇酸盐复合物的目的是获得基本有关结构，光谱和反应性能的信息包含金属半胱氨酸相互作用的蛋白质。金属半胱氨酸连接模式发生在多种金属蛋白中。我们打算准备多个含有金属硫酸盐的合成类似物蛋白质；特别是那些包含：铁的蛋白质（铁硫蛋白），铜（蓝色铜和细胞色素C氧化酶），锌（酒精脱氢酶，天冬氨酸经氨基甲基酶和金属硫硫蛋白），），镉（金属硫蛋白）和钼（黄嘌呤氧化酶和脱氢酶，醛氧化酶，亚硫酸盐氧化酶和硝酸盐还原酶）。此外，许多金属（包括钴，尼克，镉，铁和铜）已被纳入这些蛋白质作为光谱探针。这些模型化合物的化学物理研究将用于对光谱特性以及该特性的结构和电子特征金属中心。另外，从A确定的光谱参数多种金属 - 硫醇酸盐模型化合物应该对生化主义者有用为了确定不同类型的金属半胱氨酸协调模式。