CELL SPECIFIC COHESION IN D. DISCOIDEUM

盘状 D. 中的细胞特异性粘附

• 批准号: 3285810
• 负责人: CLAUDETTE KLEIN
• 金额: $ 14.23万
• 依托单位: SAINT LOUIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-05 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3285810
• 关键词: Myxomycophyta; affinity chromatography; antigens; cell adhesion; cell aggregation; cell cell interaction; cell motility; complementary DNA; contact inhibition; enzyme mechanism; flow cytometry; gene complementation; gene expression; genetic transcription; genetic translation; histogenesis; immunoprecipitation; laboratory mouse; laboratory rabbit; membrane proteins; messenger RNA; monoclonal antibody; mutagen testing; nucleic acid probes; protein biosynthesis; radioimmunoassay; site directed mutagenesis; tissue /cell culture; Myxomycophyta; affinity chromatography; antigens; cell adhesion; cell aggregation; cell cell interaction; cell motility; complementary DNA; contact inhibition; enzyme mechanism; flow cytometry; gene complementation; gene expression; genetic transcription; genetic translation; histogenesis; immunoprecipitation; laboratory mouse; laboratory rabbit; membrane proteins; messenger RNA; monoclonal antibody; mutagen testing; nucleic acid probes; protein biosynthesis; radioimmunoassay; site directed mutagenesis; tissue /cell culture

Metazoan development is characterized by the specialization of cells which, functioning in harmony, allow for the growth and survival of the organism as a whole. The ultimate goal of this research is to understand the components which regulate the spacial and temporal organization of a multicellular organism. Toward this goal, we propose to continue studies of 117 antigen, a surface protein apparently involved in cell cohesion of D. discoideum amoebae. The Specific Aims are directed toward tow complementary objectives. One is to define the antigen in biochemical terms and thus understand the features which contribute to its cell surface expression and function. The other is to understand the role of the antigen in morphogenesis. The specific Aims are to: 1. Characterize the PI-G tail of 117 antigen. 2. Examine the endogenous enzyme activity responsible for the release of 117 antigen and its physiological role. 3. Study 117 antigen developmental regulation and function. We have documented that 117 antigen possess a number of unusual modifications, including a glycolipid structure (PI-G tail) that anchors the protein to the cello surface. The biological significance of that structure is realized by the presence of an endogenous enzyme capable of releasing the antigen into the medium as part of the cells developmental cycle. This developmental cycle involves the cyclic expression of 117 antigen, underscoring its importance in morphogenesis. Continued studies of the antigen are expected to expand significantly our knowledge of how cells regulate surface levels of specific proteins and do so in a cyclic manner. This plasticity of expression reflects a more usual mechanism of regulation which may be fundamental to the morphogenesis of a multicellular organism.

内唑开发的特征是专业化 和谐功能的细胞可以增长和 整个生物的生存。 最终目标 研究是了解调节空间的组件 以及多细胞生物的时间组织。 走向这个 目标，我们建议继续研究117抗原，表面 蛋白质显然参与了D. discoideum的细胞凝聚力 变形虫。 具体目的是针对牵引互补的 目标。 一种是用生化术语定义抗原和 因此了解有助于其细胞表面的特征 表达和功能。 另一个是了解 形态发生中的抗原。 具体目的是： 1。表征117抗原的Pi-G尾巴。 2。检查负责的内源性酶活性 释放117抗原及其生理作用。 3。研究117抗原发育调节和功能。 我们已经记录了117个抗原具有许多不寻常的 修饰，包括糖脂结构（PI-G尾巴） 将蛋白质锚定在大提琴表面。 生物学 该结构的重要性是通过存在的 能够将抗原释放到培养基中的内源性酶 作为细胞发育周期的一部分。 这个发展周期 涉及117抗原的循环表达，强调其 形态发生的重要性。 对抗原的持续研究是 期望我们对细胞的了解大大扩展 调节特定蛋白质的表面水平，并在循环中这样做 方式。 这种表达的可塑性反映了更常见的 监管机制可能是对 多细胞生物的形态发生。