CHARACTERIZATION OF TRANSVERSE TUBULE MG2+-ATPASE

横管 MG2 -ATP 酶的表征

基本信息

批准号：
3071412
负责人：
TERENCE LEE KIRLEY
金额：
$ 6.82万
依托单位：
UNIVERSITY OF CINCINNATI
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-07-01 至 1996-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3071412
关键词：
adenosinetriphosphatase affinity labeling complementary DNA enzyme structure guinea pigs laboratory rabbit magnesium membrane proteins membrane transport proteins microtubule associated protein molecular biology monoclonal antibody muscle contraction muscle function muscular dystrophy nucleic acid probes protein sequence striated muscles

项目摘要

The proposed work will be on the structure and function of the Mg2+-ATPase located in the transverse tubular membrane system of mammalian skeletal muscle. The physiological function of this protein is unknown, but it is clearly structurally unrelated to the well characterized cation transporting ATPases also present in skeletal muscle. The long term goals are to better understand the role of the transverse tubules in excitation/contraction coupling and to understand the general principles underlying the structural basis for function among the class of proteins known as ATPases. The immediate goals are the elucidation of the structure and function of the transverse tubule Mg2+-ATPase. The work on the Mg2+-ATPase will, involve the identification, purification, and affinity labelling of the protein(s) involved, as well as the elucidation of possible physiological functions. Polyclonal antibodies will be raised to immunolocalize the Mg2+-ATPase. N-terminal as well as internal protein sequence will be obtained on all putative sub-units, and the entire amino acid sequence will be deduced from the cDNA sequence. Models of the structure of the Mg2+-ATPase will be proposed and the structure compared to other possibly related proteins. This work will generate important information about a component of the transverse tubule membrane system, the Mg2+-ATPase, and its relationship to the process of excitation/contraction coupling, a very important yet poorly understood physiological process.

拟议的工作将是关于 Mg2+-ATPase 的结构和功能位于哺乳动物骨骼的横向管状膜系统中肌肉。该蛋白质的生理功能尚不清楚，但它是明显在结构上与已明确表征的阳离子无关运输ATP酶也存在于骨骼肌中。长期目标是为了更好地了解横管的作用兴奋/收缩耦合并理解一般原理蛋白质类别功能的结构基础称为ATP酶。近期目标是阐明结构和横管 Mg2+-ATP 酶的功能。关于的工作 Mg2+-ATPase 将涉及鉴定、纯化和亲和力所涉及蛋白质的标记，以及阐明可能的生理功能。多克隆抗体将被提高到免疫定位 Mg2+-ATPase。 N-末端以及内部蛋白质序列将在所有推定的亚基上获得，并且整个氨基酸序列将从cDNA序列推导出来。型号将提出 Mg2+-ATPase 的结构，并将该结构与其他可能相关的蛋白质。这项工作将产生重要有关横管膜系统的一个组成部分的信息， Mg2+-ATP酶及其与兴奋/收缩过程的关系耦合，一个非常重要但知之甚少的生理过程。