UVR8 photoreceptor action and regulation

UVR8光感受器的作用和调节

基本信息

批准号：
BB/T003251/1
负责人：
Gareth Islwyn Jenkins
金额：
$ 58.89万
依托单位：
University of Glasgow
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2019
资助国家：
英国
起止时间：
2019 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FT003251%2F1
关键词：
UVR8 photoreceptor action regulation

项目摘要

Ultraviolet-B (UV-B) radiation is a minor but very energetic component of sunlight. Exposure to UV-B wavelengths (280-315 nm) has numerous effects on plants, including changes in metabolism and development that impact on biochemical composition, plant architecture and defence. The effects of UV-B are due to its ability to regulate the expression of numerous plant genes, including those involved in metabolic pathways, UV-protection, morphogenesis and defence against pests and pathogens. It is therefore important to understand how UV-B is perceived by plants and how it initiates responses. A key component involved in these processes is a protein called UVR8, which acts as a photoreceptor to detect UV-B light. UVR8 physically interacts with other proteins, COP1 and a number of transcription factors, to initiate responses to UV-B in plants. Further proteins, termed RUP proteins repress UVR8 activity. However, we have little understanding of the molecular mechanisms that determine how UVR8 interacts differentially with these proteins to control its activity, although we know that it involves a particular region of the UVR8 protein. Hence the aim of this project is to understand how UVR8 interacts with key regulatory proteins. In addition to establishing the molecular basis of UVR8 action, the information gained opens up the possibility of identifying variants of UVR8 and its interacting proteins that would allow manipulation of UVR8 activity in crop plants to enhance aspects of quality and productivity.

紫外线 B (UV-B) 辐射是阳光中含量较少但能量非常高的成分。暴露于 UV-B 波长（280-315 nm）对植物有多种影响，包括影响生化成分、植物结构和防御的新陈代谢和发育变化。 UV-B 的作用源于其调节众多植物基因表达的能力，包括涉及代谢途径、紫外线防护、形态发生以及害虫和病原体防御的基因。因此，了解植物如何感知 UV-B 以及它如何引发反应非常重要。这些过程中涉及的一个关键成分是一种名为 UVR8 的蛋白质，它充当检测 UV-B 光的光感受器。 UVR8 与其他蛋白质、COP1 和许多转录因子发生物理相互作用，启动植物对 UV-B 的反应。其他蛋白质，称为 RUP 蛋白质，可抑制 UVR8 活性。然而，我们对决定 UVR8 如何与这些蛋白质差异性相互作用以控制其活性的分子机制知之甚少，尽管我们知道它涉及 UVR8 蛋白质的特定区域。因此，该项目的目的是了解 UVR8 如何与关键调节蛋白相互作用。除了建立 UVR8 作用的分子基础外，获得的信息还为识别 UVR8 及其相互作用蛋白的变体提供了可能性，这些变体将允许操纵作物中的 UVR8 活性，以提高质量和生产力。