Defining the scope and components of ubiquitin-dependent chloroplast-associated protein degradation
定义泛素依赖性叶绿体相关蛋白降解的范围和组成部分
基本信息
- 批准号:BB/V007300/1
- 负责人:
- 金额:$ 83.18万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2021
- 资助国家:英国
- 起止时间:2021 至 无数据
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
The human population is growing rapidly and set to exceed 9bn by 2050. This presents significant challenges to food security, and places ever increasing pressure on natural resources. Thus, the drivers for increased crop yields with resilience to sub-optimal growing conditions are stronger than ever. To meet these demands it will be essential to develop improved crops. Through research on the model plant thale cress, we recently made some significant breakthroughs: We discovered a new regulatory process, named "CHLORAD", that controls vital aspects of plant growth, including plant responses to environmental stresses like drought and salinity. Significantly, modifying CHLORAD activity makes plants more tolerant of such stresses. In this project, we will define the molecular targets and mechanisms of CHLORAD, and in so doing develop a better understanding of how it can be used to deliver novel crop improvement strategies.CHLORAD (which stands for "chloroplast-associated protein degradation") regulates the development and operation of structures inside plant cells called chloroplasts, which are normal cellular constituents (i.e., organelles). They define plants, contain the green pigment chlorophyll, and are responsible for photosynthesis, harnessing sunlight energy to power the activities of the cell and the growth of the plant. As photosynthesis is the only significant mechanism of energy-input into the living world, chloroplasts are of huge importance, not just to plants but to all life on Earth. Chloroplasts also have critical roles in plant responses to stress, and so are ideal targets for engineering resilient crops.Chloroplasts are composed of thousands of different proteins, most of which are encoded by genes in the cell nucleus and so are made outside of the organelle in the cellular matrix known as the cytosol. As chloroplasts are surrounded by a double-membrane "envelope", sophisticated machinery is needed to enable the import of these proteins into the organelle. This comprises molecular machines in both membranes, called TOC (for "Translocon at the Outer membrane of Chloroplasts") and TIC. Each machine is composed of several proteins that work cooperatively.Currently, CHLORAD is known to act on the TOC machinery, breaking up its constituent proteins in order to control which other proteins are imported into the organelle (this in turn influences organelle development and operation). Known components of the CHLORAD machinery are proteins called SP1, SP2 and CDC48. The first, SP1, is a "ubiquitin E3 ligase" that labels-up unwanted proteins to target them for removal. The SP2 protein forms a channel in the chloroplast outer membrane, providing the exit route for removal of proteins labelled by SP1. Lastly, CDC48 is a molecular motor that drives extraction of the unwanted to the cytosol, where they are then broken down.Our unpublished results have revealed that additional proteins (or "cofactors") work together with CDC48 in CHLORAD. We believe that these cofactors are required for the docking of CDC48 onto the unwanted TOC proteins, and for the subsequent release of those proteins from CDC48. Here, we will study these cofactors in detail, to better understand how unwanted chloroplast proteins are removed in CHLORAD. Furthermore, we have additional new results showing that CHLORAD acts on a much larger number of target proteins than previously envisaged (i.e., not just TOC proteins), including proteins of the chloroplast interior. We will systematically identify these novel targets, and seek to understand how they are processed by CHLORAD even when deep inside the organelle. Informed by information on the identity of the targets, we will also explore the broader physiological significance of CHLORAD, for plant growth and development.Overall, the knowledge gained will enhance our understanding of CHLORAD, and will be important for the development of crops with improved chloroplast performance.
到2050年,人口正在迅速增长,并将超过90亿。这给粮食安全带来了重大挑战,并且对自然资源的压力越来越大。因此,抗次最佳生长条件的抗性产量增加的驱动因素比以往任何时候都更强。为了满足这些要求,开发改进的作物至关重要。通过对模型植物Thale Cress的研究,我们最近做出了一些重大突破:我们发现了一个名为“氯拉德”的新调节过程,该过程控制着植物生长的重要方面,包括植物对诸如干旱和盐度等环境压力的反应。值得注意的是,修饰氯拉德活性会使植物更耐受这种应激。在这个项目中,我们将定义氯化氯化藻的分子靶标和机制,并因此可以更好地理解它如何用于提供新的农作物改进策略。氯化(代表“叶绿体相关蛋白质降解”代表植物细胞中植物细胞的开发和操作,植物性细胞的开发和操作称为叶绿体,称为蜂窝状构成。他们定义植物,含有绿色色素叶绿素,并负责光合作用,利用阳光能量来为细胞的活性和植物的生长提供动力。由于光合作用是对生活世界的能量输入的唯一重要机制,因此叶绿体非常重要,不仅对植物,而且对地球上的所有生命。叶绿体在植物对胁迫的反应中也具有关键作用,因此,工程弹性作物的理想靶标也是由数千种不同的蛋白质组成,其中大多数由细胞核中的基因编码,因此在细胞核中的细胞基质中,在细胞核中已知的细胞溶液中已知。由于叶绿体被双膜“包络”包围,因此需要精致的机械以使这些蛋白质将这些蛋白质进口到细胞器中。这包括两个膜中的分子机,称为TOC(用于“叶绿体外膜”)和TIC。每台机器都由几种合作起作用的蛋白质组成。目前,氯拉德众所周知,氯拉德会作用于TOC机械,分解其组成蛋白,以控制哪些其他蛋白质被进口到细胞器中(这反过来又影响了细胞器的发育和操作)。氯拉德机械的已知成分是称为SP1,SP2和CDC48的蛋白质。第一个是SP1,是一种“泛素E3连接酶”,标记不需要的蛋白质以靶向去除。 SP2蛋白在叶绿体外膜中形成通道,为去除SP1标记的蛋白质提供了出口途径。最后,CDC48是一种分子运动,可驱动不需要的细胞质萃取,然后分解它们。我们未发表的结果表明,其他蛋白质(或“辅助因子”)与氯拉德中的CDC48一起起作用。我们认为,这些辅助因子是将Cdc48对接到不需要的TOC蛋白上以及随后从CDC48中释放的这些蛋白所必需的。在这里,我们将详细研究这些辅助因子,以更好地了解如何在氯拉德中去除不必要的叶绿体蛋白。此外,我们还有其他新的结果表明,氯腺比以前设想的(即,不仅是TOC蛋白)(包括叶绿体内部的蛋白质)作用要大得多的靶蛋白。我们将系统地确定这些新颖的目标,并试图了解即使在细胞器内部的深处,藻类也是如何处理的。通过有关目标身份的信息,我们还将探索氯化氯化氯化藻的更广泛的生理意义,对于植物的生长和发育。综上所述,获得的知识将增强我们对氯化氯化的理解,对于通过改善叶绿体表现的农作物的发展非常重要。
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Mutations in TIC100 impair and repair chloroplast protein import and impact retrograde signalling
TIC100 突变损害和修复叶绿体蛋白输入并影响逆行信号传导
- DOI:10.1101/2022.01.18.476798
- 发表时间:2022
- 期刊:
- 影响因子:0
- 作者:Loudya N
- 通讯作者:Loudya N
The chloroplast-associated protein degradation pathway controls chromoplast development and fruit ripening in tomato
- DOI:10.1038/s41477-021-00916-y
- 发表时间:2021-05-01
- 期刊:
- 影响因子:18
- 作者:Ling, Qihua;Sadali, Najiah Mohd;Jarvis, R. Paul
- 通讯作者:Jarvis, R. Paul
Crosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis.
- DOI:10.7554/elife.60960
- 发表时间:2021-09-02
- 期刊:
- 影响因子:7.7
- 作者:Watson SJ;Li N;Ye Y;Wu F;Ling Q;Jarvis RP
- 通讯作者:Jarvis RP
Mutations in the chloroplast inner envelope protein TIC100 impair and repair chloroplast protein import and impact retrograde signaling.
- DOI:10.1093/plcell/koac153
- 发表时间:2022-07-30
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis.
- DOI:10.1126/sciadv.abq7352
- 发表时间:2022-11-18
- 期刊:
- 影响因子:13.6
- 作者:Sun, Yi;Yao, Zujie;Ye, Yiting;Fang, Jun;Chen, Honglin;Lyu, Yuping;Broad, William;Fournier, Marjorie;Chen, Genyun;Hu, Yonghong;Mohammed, Shabaz;Ling, Qihua;Jarvis, R. Paul
- 通讯作者:Jarvis, R. Paul
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Paul Jarvis其他文献
Associations Between Physical Characteristics and Golf Clubhead Speed: A Systematic Review with Meta-Analysis.
物理特征与高尔夫球杆头速度之间的关联:荟萃分析的系统回顾。
- DOI:
10.1007/s40279-024-02004-5 - 发表时间:
2024 - 期刊:
- 影响因子:9.8
- 作者:
Alex Brennan;Andrew Murray;Margo Mountjoy;John Hellstrom;D. Coughlan;Jack Wells;Simon L Brearley;Alex Ehlert;Paul Jarvis;Anthony Turner;Chris Bishop - 通讯作者:
Chris Bishop
Intracerebral Hemorrhage in a Young Urban Population: Etiologies and Outcomes in Patients 50 and Younger
- DOI:
10.1016/j.jstrokecerebrovasdis.2019.07.011 - 发表时间:
2019-10-01 - 期刊:
- 影响因子:
- 作者:
Aron Gedansky;Paul Jarvis;Daohai Yu;Xiaoning Lu;Terry Heiman-Patterson;Guillermo Linares - 通讯作者:
Guillermo Linares
The Arabidopsis Book(control of plastid development, protein import, division and inheritance.)
拟南芥书(质体发育、蛋白质输入、分裂和遗传的控制。)
- DOI:
- 发表时间:
2008 - 期刊:
- 影响因子:0
- 作者:
Wataru Sakamoto;Shin-ya Miyagishima;Paul Jarvis - 通讯作者:
Paul Jarvis
Supporting Creativity and Appreciation of Uncertainty in Exploring Geo-coded Public Health Data
支持探索地理编码公共卫生数据时的创造力和对不确定性的认识
- DOI:
- 发表时间:
2009 - 期刊:
- 影响因子:1.7
- 作者:
S. Thew;Alistair Sutcliffe;O. Bruijn;John McNaught;Rob Procter;Paul Jarvis;Iain Buchan - 通讯作者:
Iain Buchan
Nursing and midwifery students' perceptions of spirituality, spiritual care, and spiritual care competency: A prospective, longitudinal, correlational European study
- DOI:
10.1016/j.nedt.2018.05.002 - 发表时间:
2018-08-01 - 期刊:
- 影响因子:
- 作者:
Linda Ross;Wilfred McSherry;Tove Giske;René van Leeuwen;Annemiek Schep-Akkerman;Tiburtius Koslander;Jenny Hall;Vibeke Østergaard Steenfeldt;Paul Jarvis - 通讯作者:
Paul Jarvis
Paul Jarvis的其他文献
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{{ truncateString('Paul Jarvis', 18)}}的其他基金
Defining the role of SUMO in regulating chloroplast biogenesis and functions
定义 SUMO 在调节叶绿体生物发生和功能中的作用
- 批准号:
BB/W015021/1 - 财政年份:2023
- 资助金额:
$ 83.18万 - 项目类别:
Research Grant
Uncovering how plant pathogens take control of chloroplast protein import to limit chloroplast-mediated immunity
揭示植物病原体如何控制叶绿体蛋白输入以限制叶绿体介导的免疫
- 批准号:
BB/X000192/1 - 财政年份:2023
- 资助金额:
$ 83.18万 - 项目类别:
Research Grant
Application of the plastidic E3 ligase SP1 in crop improvement, using tomato and rice as models
质体E3连接酶SP1在作物改良中的应用(以番茄和水稻为模型)
- 批准号:
BB/R005591/1 - 财政年份:2018
- 资助金额:
$ 83.18万 - 项目类别:
Research Grant
Elucidating the role of SP2 and the SP1-SP2 machinery in chloroplast protein degradation
阐明 SP2 和 SP1-SP2 机制在叶绿体蛋白质降解中的作用
- 批准号:
BB/R016984/1 - 财政年份:2018
- 资助金额:
$ 83.18万 - 项目类别:
Research Grant
Chloroplast-Associated Degradation (CHLORAD): Molecular definition of a ubiquitin-dependent system for plastid protein removal in plants
叶绿体相关降解 (CHLORAD):植物中质体蛋白去除泛素依赖性系统的分子定义
- 批准号:
BB/R009333/1 - 财政年份:2018
- 资助金额:
$ 83.18万 - 项目类别:
Research Grant
Role of the chloroplast ubiquitin E3 ligase SP1 in abiotic stress tolerance in plants
叶绿体泛素 E3 连接酶 SP1 在植物非生物胁迫耐受中的作用
- 批准号:
BB/N006372/1 - 财政年份:2016
- 资助金额:
$ 83.18万 - 项目类别:
Research Grant
Investigating the function of a ClpC/Hsp100-type chaperone in chloroplast preprotein import
研究 ClpC/Hsp100 型伴侣在叶绿体前蛋白输入中的功能
- 批准号:
BB/J017256/2 - 财政年份:2013
- 资助金额:
$ 83.18万 - 项目类别:
Research Grant
Investigating the roles of Arabidopsis STIC1 and STIC2 in chloroplast protein transport
研究拟南芥 STIC1 和 STIC2 在叶绿体蛋白转运中的作用
- 批准号:
BB/J009369/2 - 财政年份:2013
- 资助金额:
$ 83.18万 - 项目类别:
Research Grant
Control of plastid biogenesis by the ubiquitin-proteasome system
泛素-蛋白酶体系统对质体生物发生的控制
- 批准号:
BB/K018442/1 - 财政年份:2013
- 资助金额:
$ 83.18万 - 项目类别:
Research Grant
Investigating the roles of Arabidopsis STIC1 and STIC2 in chloroplast protein transport
研究拟南芥 STIC1 和 STIC2 在叶绿体蛋白转运中的作用
- 批准号:
BB/J009369/1 - 财政年份:2012
- 资助金额:
$ 83.18万 - 项目类别:
Research Grant
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