Targeting Plant Pathogens Through LPMO Gene Silencing

通过 LPMO 基因沉默靶向植物病原体

• 批准号: BB/S018735/1
• 负责人: Katherine Denby
• 金额: $ 25.78万
• 依托单位: University of York
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS018735%2F1
• 关键词: Targeting; Plant; Pathogens; Through; LPMO

We develop novel highly specific crop protection against downy mildew in lettuce and spinach, and post-harvest mould in strawberry and green beans. Host penetration by pathogens typically requires enzymatic disruption of the plant cell wall with the crystalline nature of cellulose making this challenging. Lytic polysaccharide monooxygenases (LPMOs) play a key role in lysing bonds at the crystalline surfaces of polysaccharides allowing their rapid degradation. We recently identified a new family of LPMOs in plant pathogens. Members of this family are expressed during early stages of host infection suggesting they function to degrade the cell wall and enable the pathogen to penetrate the plant. Here we will identify key LPMOs required for virulence in two oomycete and one fungal pathogens. We will optimise application of RNAi for silencing these LPMO genes and preventing infection. A business plan for scale up and commercialisation of this novel approach will be developed.

我们开发了新型高度特异性作物保护，以防止生菜和菠菜中的霜霉病以及草莓和青豆中的收获后霉菌。病原体侵入宿主通常需要酶促破坏植物细胞壁，而纤维素的结晶性质使得这一点具有挑战性。裂解多糖单加氧酶 (LPMO) 在裂解多糖晶体表面的键以使其快速降解方面发挥着关键作用。我们最近在植物病原体中发现了一个新的 LPMO 家族。该家族的成员在宿主感染的早期阶段表达，表明它们的功能是降解细胞壁并使病原体能够渗透植物。在这里，我们将确定两种卵菌和一种真菌病原体毒力所需的关键 LPMO。我们将优化 RNAi 的应用，以沉默这些 LPMO 基因并预防感染。将制定扩大这种新方法并将其商业化的商业计划。