LENS PROTEINS & FLUORESCENCE: IN VIVO AND IN VITRO STUDY

晶状体蛋白

• 批准号: 3256012
• 负责人: SIDNEY LERMAN
• 金额: $ 18.12万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-06-01 至 1991-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3256012
• 关键词: adult human (21+); aging; biophysics; cataract; fluorescence; fluorescence spectrometry; human tissue; image processing; lens proteins; mature animal; nuclear magnetic resonance spectroscopy; photochemistry; radiobiology; radiotracer; spectrometry; ultraviolet radiation

Our aim is to further delineate the relationship between UV exposure, lens aging and cataractogenesis at the molecular level and correlate these data with our other methods of evaluation (optical spectroscopy, morphology, and the CCRG photographic classification system) and with the in vivo methods we have developed (UV slit lamp densitography and surface scanning NMR spectroscopy). We have further refined the UV slit lamp densitographic technique to permit widespread clinical application. An automated program has now been developed which can measure both corneal and lenticular fluorescence levels and provide biometric data as well. This program will be further tested on at least 100 patients during the coming year. In addition to the foregoing biophysical techniques, we are expanding our efforts to perform multiprobe NMR spectroscopy on whole eyes (fresh donor specimens) and on live monkeys and rabbits. We are currently performing 23Na as well as 31P analyses and proton imaging in order to develop specific parameters for in vivo NMR spectroscopy. These two in vivo biophysical approaches will enable us to correlate changes in the living lens with our ongoing multifaceted in vitro spectroscopic analyses. We have also expanded our program in which we perform the Schiempflug photos (with the UV-visible slit lamp apparatus) on patients prior to cataract surgery, and correlate these data with our laboratory analyses on the extracted lens or lens matter from these patients. A similar pilot study involving NMR spectroscopy will be instituted this year. Aside from our ongoing studies on the effects of direct UV radiation exposure on the ocular lens, we are continuing our investigations on (UV) photosensitized damage as exemplified by our work on the Psoralens and Allopurinol. The biophysical (fluorescence) in vivo approach is being applied to such patients, and our in vitro spectroscopic analysis (involving fluorescence, phosphorescence, and NMR spectroscopy) are being utilized to further delineate the types of mechanisms involved in such damage (Type I or Type II reaction).

我们的目的是进一步描述紫外线，镜头之间的关系 分子水平上的衰老和白内生生成并将这些数据相关联 使用我们的其他评估方法（光谱，形态和 CCRG摄影分类系统）和体内方法 我们已经开发了（紫外缝灯光密摄影和表面扫描NMR 光谱法）。 我们进一步完善了紫外缝灯光密度学 允许广泛临床应用的技术。 一个自动程序 现在已经开发了可以测量角膜和凸状的 荧光水平并提供生物特征数据。 这个程序将 在来年，至少对100名患者进行进一步测试。 在 除了上述生物物理技术，我们正在扩大我们的 努力在整个眼睛上执行多功能NMR光谱法（新鲜供体 标本）以及活猴子和兔子。 我们目前正在执行 23NA以及31p分析和质子成像以发展 体内NMR光谱的特定参数。 这两个体内 生物物理方法将使我们能够关联生活的变化 我们正在进行的多面体外光谱分析的镜头。 我们 还扩展了我们执行Schiempflug照片的程序 （带有紫外可见的缝隙灯设备）在白内障之前的患者 手术，并将这些数据与我们的实验室分析相关联 从这些患者中提取的镜头或镜头很重要。 类似的试点研究 涉及NMR光谱法将于今年进行。 除了我们 正在进行的有关直接紫外线辐射暴露对 眼镜镜头，我们正在继续对（UV）光敏的调查 我们在牛cor和别嘌醇上的工作所示的损害。 这 生物物理（荧光）在体内方法正在应用于此类 患者和我们的体外光谱分析（涉及荧光， 磷光和NMR光谱法被用于进一步 描述这种损坏所涉及的机制类型（I型或类型 II反应）。