Auxentric - a hormone-based mechanism to control chromatin state

Auxentric - 一种基于激素的控制染色质状态的机制

基本信息

批准号：
BB/S002901/1
负责人：
Lars Ostergaard
金额：
$ 69.58万
依托单位：
John Innes Centre
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2019
资助国家：
英国
起止时间：
2019 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FS002901%2F1
关键词：
Auxentric hormone based mechanism control

项目摘要

The fate of all cells, whether unicellular or part of a multicellular organism, is a function of the genes they express. During the development of multicellular organisms, mobile molecules such as hormones regulate cell fate by controlling gene expression. The plant hormone auxin was one of the first hormones to be studied and the effect of auxin on light-regulated plant growth (phototropism) was investigated by Charles Darwin and his son Francis in the 1880s. It was, however, not until the 1930s that the auxin molecule was isolated and its molecular structure determined as indole 3-acetic acid (IAA). In plants, auxin plays an essential role in initiating organ formation and in patterning the organs in specific tissue types, for example, lateral roots, young leaves and the gynoecium (the female reproductive organ). In the classical auxin-signalling mechanism, the auxin molecule promotes the interaction between specific proteins thereby causing the breakdown of repressors of gene expression. It has also been established that auxin can influence its own transport by controlling the localisation of auxin transporters. Although these mechanisms of auxin signalling can explain many processes of auxin action, other transcriptional signalling pathways are likely to exist to account for the multitude of processes in which auxin plays a role. We have recently described an alternative auxin-signalling pathway mediated by the auxin response factor, ETTIN, which plays a particularly important role during the establishment of polarity in organ development. This novel auxin-signalling mechanism (referred to here as the 'Auxentric' mechanism) is fundamentally different from established processes of auxin signalling as it involves a direct effect of the hormone molecule on ETTIN-transcription factor (TF) complexes without the involvement of protein degradation. Interestingly, our preliminary data suggest that this effect leads to changes in chromatin states in a mechanism similar to thyroid hormone signalling in animals. In this proposal, we will reveal the mechanism by which auxin mediates its effect on ETTIN-containing complexes to control gene expression. We will, moreover, unravel the biophysical and structural characteristics of the protein and hormonal components involved. Above and beyond this specific mechanism, Auxentric may have far-reaching implications for the existence of alternative mechanisms by which hormones regulate plant growth and development. The work proposed here will thus introduce a novel gene expression-based mechanism of hormone perception in plants and draw unprecedented links between auxin dynamics and gene regulation at the chromatin level.

所有细胞的命运，无论是单细胞生物的一部分还是一部分多细胞生物，都是它们表达的基因的函数。在多细胞生物的发展过程中，移动分子（例如激素）通过控制基因表达来调节细胞命运。植物激素生长素是首先要研究的激素之一，生长素对1880年代的查尔斯·达尔文（Charles Darwin）和他的儿子弗朗西斯（Francis）研究了生长素对光调节植物生长（光质主义）的影响。但是，直到1930年代，生长素分子才被分离出来，其分子结构确定为吲哚3-乙酸（IAA）。在植物中，生长素在启动器官形成和对特定组织类型的器官构图中起着至关重要的作用，例如，侧根，幼叶和妇科（女性生殖器官）。在经典的生长素 - 信号机制中，生长素分子促进了特定蛋白质之间的相互作用，从而导致基因表达的阻遏物分解。还已经确定生长素可以通过控制生长素转运蛋白的定位来影响其自身的运输。尽管这些生长素信号传导的这些机制可以解释生长素作用的许多过程，但其他转录信号传导途径可能存在，以说明生长素发挥作用的多种过程。我们最近描述了由生长素反应因子Ettin介导的一种替代生长素 - 信号途径，该途径在器官发育中在极性建立过程中起着特别重要的作用。这种新型的生长素信号机制（此处称为“辅助”机制）与生长素信号传导的既定过程根本不同，因为它涉及激素分子对ettin-转录因子（TF）络合物的直接作用，而无需蛋白质降解。有趣的是，我们的初步数据表明，这种作用会导致染色质状态的变化，该机制类似于动物的甲状腺激素信号传导。在此提案中，我们将揭示生长素介导其对含Ettin的复合物的影响以控制基因表达的机制。此外，我们将揭示所涉及的蛋白质和激素成分的生物物理和结构特征。除了这种特定机制之外，辅助可能对激素调节植物生长和发育的替代机制的存在可能具有深远的影响。因此，这里提出的工作将引入一种基于基因基因表达的基于植物中激素感知的基因表达机制，并在染色质水平下生长素动力学和基因调节之间存在前所未有的联系。