METAL CHELATION IN METALLOTHIONEIN

金属硫蛋白中的金属螯合

• 批准号: 3251533
• 负责人: Dennis R. Winge
• 金额: $ 12.2万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-05-01 至 1989-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3251533
• 关键词: Escherichia coli; antifungal agents; bacterial genetics; clone cells; double stranded RNA; food poisoning; genetic transcription; genetic translation; genome; laboratory rabbit; messenger RNA; metal complex; metallothionein; microorganism genetics; mycotoxins; nucleic acid sequence; virus envelope

Among the 17 mycoviruses which have been physically characterized, there are two which code for specific toxins lethal to the non-infected strains of the same fungi. These are the mycoviruses Saccharomyces cerevisiae and Ustilago maydis. These viruses have many similar properties, including segmented dsRNA genomes which may be grouped into classes of heavy (4-6 kbp), medium (0.7-1.4 kbp) and light (less than 0.35 kbp) segments. In both viruses the heavy segments code for capsid production and the medium segments code for toxins. There are three strains of the Ustilago virus which are distinguishable by the number of genomic segments of each class and slight differences in specificity of the toxins. In this project relationships of the dsRNA segments within and among the three strains will be determined using heterodupliex and hybridization procedures. The ability of each segment to be transcribed to mRNA and the translation product of representative dsRNA segments will be determined. Specific dsRNA segments will be cloned in E. coli for the study of amplified gene products and sequence analysis. These studies will provide a better understanding of how toxins coded by viruses interrupt or relate to host cell metabolism. Such basic knowledge will be used in the control of pathogenic fungi or the control of specific toxins secreted by fungi which result in food poisoning.

在物理表征的17种菌病毒中， 是两个针对未感染菌株的特异性毒素代码的代码 同样的真菌。 这些是酿酒酵母的葡萄球病毒病毒和 Ustilago Maydis。 这些病毒具有许多相似的特性，包括 分段的DSRNA基因组可以分为重量（4-6 KBP），培养基（0.7-1.4 kbp）和光（小于0.35 kbp）段。 在 这两种病毒均可用于封闭式生产和培养基的重型细分市场代码 毒素的片段代码。 乌斯利戈病毒有三种菌株 每个类的基因组段数量可以区分 以及毒素特异性的略有差异。 在这个项目中 DSRNA段内部和三个菌株之间的关系将 使用杂化和杂交程序确定。 这 每个段的能力被转录为mRNA和翻译 将确定代表性DSRNA段的乘积。 具体的 DSRNA段将在大肠杆菌中克隆以进行扩增基因 产品和序列分析。 这些研究将提供更好的 了解病毒编码的毒素如何中断或与宿主有关 细胞代谢。 这样的基本知识将用于控制 致病真菌或对真菌分泌的特定毒素的控制 导致食物中毒。