GENE REGULATION OF THE CYTOKINES IL-3 AND GM-CSF

细胞因子 IL-3 和 GM-CSF 的基因调控

• 批准号: 3242636
• 负责人: BERNARD MATHEY-PREVOT
• 金额: $ 19.23万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3242636
• 关键词: DNA binding protein; RNase protection assay; T lymphocyte; cell growth regulation; cell type; chromatin; clone cells; colony stimulating factor; complementary DNA; cytokine; fibroblasts; gel mobility shift assay; gene expression; genetic regulation; genetic transcription; granulocyte; hematopoietic stem cells; immunogenetics; interleukin 3; ion exchange chromatography; methylation; molecular cloning; neurons; nucleic acid sequence; nucleoproteins; oligonucleotides; pancreatic ribonuclease; transcription factor; transfection

The proliferation and maturation of hematopoietic stem and progenitor cells is strongly affected by the availability and supply of hematopoietic growth factors, particularly interleukin-3 (IL3) and granulocyte-macrophage colony simulating factor (GM-CSF). As a result, regulation of IL-3 and GM-CSF gene expression will weigh heavily in the process of hematopoietic differentiation. To gain some insights into the molecular events underlying their regulation, IL-3 and GM-CSF gene expression will be first be examined in a model cell line, MLA 144 gibbon T leukemic cells. It will subsequently be broadened to human T lymphocytes, macrophages and fibroblasts. Two broad issues will be addressed. First, experiments are described to identify DNA regions of IL-3 and GM-CSF genes responsible for transcriptional activation, and to characterize and clone nuclear factors which interact with such regions. Transient transfection and RNase protection assays will be performed to identify the regulatory DNA regions which confer transcriptional activation of the cytokine genes. These DNA sequences will be used to identify nuclear factors in gel retardation assays, and various criteria will be applied to characterize the nuclear protein-DNA complexes, including DNA sequence specificity, DNA footprinting, and methylation interference. Mutagenesis of the contact points between DNA and the bound factors will be performed and its effect on DNA binding and transcriptional activation of reporter genes in which the mutated motif replaces wild-type sequences will be assessed. Nuclear factors which are confirmed to participate in the regulation of expression of the two cytokines will be cloned by screening cDNA expression libraries with oligonucleotides consisting of tandem repeats of the bound DNA sequence, or by direct expression in COS cells and gel retardation assay. Also, the nature and role of jun and fos (or their related proteins) will be evaluated in the activation of the IL-3 gene. Second, we will investigate in molecular terms how expression of IL-3 and GM-CSF, in contrast to T cells, is uncoupled in macrophage and fibroblast cells. Chromatin structure, promoter activities and DNA nuclear proteins will be compared and characterized in the different cell types using the same techniques listed earlier. Molecular cloning of new nuclear factors, if identified, will also be carried out. In addition to these topics, the possibility of IL-3 expression by human neuronal cells will be investigated by conventional RNA analyses.

造血干和祖细胞的增殖和成熟 受造血生长的可用性和供应的强烈影响 因素，尤其是白介素3（IL3）和粒细胞 - 巨噬细胞菌落 模拟因子（GM-CSF）。结果，IL-3和GM-CSF基因的调节 表达在造血的过程中会严重称重 分化。为了获得一些对分子事件基础事件的见解 他们的调节IL-3和GM-CSF基因表达将首先进行检查 在模型细胞系中，MLA 144头g型白血病细胞。随后会 扩展到人T淋巴细胞，巨噬细胞和成纤维细胞。两个宽 问题将被解决。首先，描述了识别DNA的实验 IL-3和GM-CSF基因的区域负责转录 激活，并表征和克隆核因子相互作用 与这样的地区。瞬态转染和RNase保护分析将 进行以确定授予的调节DNA区域 细胞因子基因的转录激活。这些DNA序列将 用于识别凝胶延迟测定中的核因子，并 标准将用于表征核蛋白-DNA复合物， 包括DNA序列特异性，DNA足迹和甲基化 干涉。 DNA与结合之间的接触点的诱变 将执行因素及其对DNA结合和转录的影响 突变基序替代野生型的报告基因的激活 序列将被评估。核因素已确认 参与两种细胞因子表达的调节将是 通过用寡核苷酸筛选cDNA表达文库克隆 由结合DNA序列的串联重复或直接组成 COS细胞和凝胶延迟测定中的表达。此外，自然和 JUN和FOS（或其相关蛋白质）的作用将在 IL-3基因的激活。其次，我们将以分子的方式进行调查 与T细胞相比，IL-3和GM-CSF的表达如何在 巨噬细胞和成纤维细胞。染色质结构，启动子活动 和DNA核蛋白将被比较并在 使用前面列出的相同技术的不同单元类型。分子 如果鉴定出新的核因素的克隆也将进行。在 除了这些主题，人类表达IL-3的可能性 神经元细胞将通过常规RNA分析研究。