CYTOCHEMISTRY OF CENTRAL AUDITORY PATHWAYS

中枢听觉通路的细胞化学

基本信息

批准号：
3217415
负责人：
RICHARD L SAINT MARIE
金额：
$ 11.76万
依托单位：
HOUSE RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
1992
资助国家：
美国
起止时间：
1992-09-01 至 1994-02-28
项目状态：
已结题

项目摘要

Binaural processing is important because it allows organisms to localize sounds in their environment. Evidence of this processing can be found at early levels of central auditory pathways in mammals, including nuclei of the superior olive, lateral lemniscus, and inferior colliculus. Neurons in these pathways use interaural time and level differences (ITD's and ILD'S) to compute the source of a sound. Part of this processing involves the interaction of excitatory inputs driven by one ear and inhibitory inputs driven by the other ear. In this project we will investigate which neurons in the auditory pathways provide inhibitory inputs for binaural processing, and how these inputs converge with excitatory inputs at different levels of the auditory pathway. We will relate these findings to the processing of ITD's and ILD's in nuclei of the superior olive, lateral lemniscus, and inferior colliculus. To do this, immunocytochemistry and transmitter-specific uptake will be used to identify which neurons in the nuclei of the superior olive, lateral lemniscus, and inferior colliculus may use inhibitory or excitatory transmitters. These procedures will be combined with retrograde transport of exogenously injected tracers to identify inhibitory or excitatory neurons with ascending projections. Moreover, anterograde tracing of the afferent inputs to these projection neurons will be combined with retrograde transport and immunocytochemistry. In this way we will define the chains of neurons and their inhibitory connections which are likely to be involved in the binaural processing of ITD's and ILD'S.

双耳处理很重要，因为它允许生物本地化听起来在他们的环境中。可以在哺乳动物中央听觉途径的早期水平，包括上橄榄，外侧lemniscus和下丘。神经元在这些途径使用跨层次和水平差异（ITD和ILD）计算声音的来源。该处理的一部分涉及由一只耳朵和抑制性输入驱动的兴奋投入的相互作用由另一只耳朵驱动。在这个项目中，我们将调查哪些神经元在听觉途径中，可提供双耳处理的抑制投入，以及这些输入如何通过不同级别的兴奋性输入收敛听觉路径。我们将将这些发现与处理 ITD和ILD在上级橄榄，外侧Lemniscus和下丘。为此，免疫细胞化学和发射器特异性摄取将是用于识别上橄榄核中哪些神经元 Lemniscus和下丘可能会使用抑制或兴奋性发射器。这些程序将与逆行运输结合外源注射的示踪剂以识别抑制性或兴奋性具有上升预测的神经元。此外，对这些投影神经元的传入输入将与逆行运输和免疫细胞化学。这样我们将定义神经元的链及其抑制连接可能参与ITD和ILD的双耳处理。