14-ERASynBio - IESY - Inducible Evolution of Synthetic Yeast genomes
14-ERASynBio - IESY - 合成酵母基因组的诱导进化
基本信息
- 批准号:BB/M005690/2
- 负责人:
- 金额:$ 10.12万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2017
- 资助国家:英国
- 起止时间:2017 至 无数据
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Induced Evolution of Synthetic Yeast genomes (IESY) will use the first synthetic eukaryote, Saccharomyces cerevisiae 2.0 (Sc2.0), as a platform for metabolic engineering and genome minimization, and more importantly for generating and understanding industrially high-value phenotypes. Synthetic chromosomes in Sc2.0 permit rapid and comprehensive genome evolution through synthetic chromosome rearrangement and modification by loxP-mediated evolution (SCRaMbLE). SCRaMbLE will be exploited here to evolve strains selected for high-value phenotypes for biofuels and biotechnology, using both chemostats and batch transfer methods in the USA and Europe. Technologies for neochromosomes and orthogonal SCRaMbLE of gene classes will be developed. Evolutionary trajectories will be analysed to relate genome structure with genome function: DNA sequencing will reveal the genome sequence, rearrangements, and copy number changes in the evolved strains; chromosome conformation capture will show how massive rearrangements affect 3D structure; and deep sequencing technologies will relate sequence and structure to gene expression and isoform abundance. Computational analysis will identify the evolutionary drivers for high fitness, with the potential for further optimization. IESY builds on resources uniquely available from the international Sc2.0 consortium and will be an international resource for efficient evolution of high-value phenotypes. This project represents a new paradigm in synthetic biology in which a genome is pre-programmed to explore combinatorial diversity space to evolve new and useful function.
诱导的合成酵母基因组(IESY)的演变将使用第一个合成真核生物酿酒酵母2.0(SC2.0)作为代谢工程和基因组最小化的平台,更重要的是生成和理解工业高价值表型。 SC2.0中的合成染色体可以通过LOXP介导的进化(Scramble)进行合成染色体重排和修饰,可以快速而全面的基因组进化。在这里,将使用Chemofule和生物技术的高价值表型,使用Chemodotats和Chemotatats和批处理转移方法在这里开发争夺,以进化为生物燃料和生物技术的高价值表型的发展。将开发针对基因类别的新染色体和正交拼凑的技术。将分析进化轨迹,以将基因组结构与基因组功能相关联:DNA测序将揭示进化菌株中的基因组序列,重排和拷贝数变化;染色体构象捕获将显示大量重排如何影响3D结构;深层测序技术将序列和结构与基因表达和同工型丰度相关联。计算分析将确定高健身的进化驱动因素,并具有进一步优化的潜力。 Iesy建立在国际SC2.0联盟中独特获得的资源基础上,将成为有效发展高价值表型的国际资源。该项目代表了合成生物学的一个新范式,其中预先编程了基因组来探索组合多样性空间以发展新的和有用的功能。
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Designing with living systems in the synthetic yeast project.
- DOI:10.1038/s41467-018-05332-z
- 发表时间:2018-07-27
- 期刊:
- 影响因子:16.6
- 作者:Szymanski E;Calvert J
- 通讯作者:Calvert J
Synthetic genomics: a new venture to dissect genome fundamentals and engineer new functions.
- DOI:10.1016/j.cbpa.2018.04.002
- 发表时间:2018-10
- 期刊:
- 影响因子:7.8
- 作者:Schindler D;Dai J;Cai Y
- 通讯作者:Cai Y
Genetic Constructor: An Online DNA Design Platform.
Genetic Constructor:在线 DNA 设计平台。
- DOI:10.1021/acssynbio.7b00236
- 发表时间:2017
- 期刊:
- 影响因子:4.7
- 作者:Bates M
- 通讯作者:Bates M
Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods.
使用体外和体内合成基因组 SCRaMbLE-in 方法进行快速途径原型设计和工程设计
- DOI:10.1038/s41467-018-04254-0
- 发表时间:2018-05-22
- 期刊:
- 影响因子:16.6
- 作者:Liu W;Luo Z;Wang Y;Pham NT;Tuck L;Pérez-Pi I;Liu L;Shen Y;French C;Auer M;Marles-Wright J;Dai J;Cai Y
- 通讯作者:Cai Y
Leaf LIMS: A Flexible Laboratory Information Management System with a Synthetic Biology Focus.
Leaf LIMS:以合成生物学为重点的灵活实验室信息管理系统。
- DOI:10.1021/acssynbio.7b00212
- 发表时间:2017
- 期刊:
- 影响因子:4.7
- 作者:Craig T
- 通讯作者:Craig T
共 7 条
- 1
- 2
Yizhi Cai其他文献
Identifing and characterizing SCRaMbLEd synthetic yeast using ReSCuES
使用 ReSCuES 识别和表征 SCRaMbLED 合成酵母
- DOI:
- 发表时间:20182018
- 期刊:
- 影响因子:16.6
- 作者:Zhouqing Luo;Lihui Wang;Yun Wang;Weimin Zhang;Yakun Guo;Yue Shen;Linghuo Jiang;Qingyu Wu;Chong Zhang;Yizhi Cai;Junbiao DaiZhouqing Luo;Lihui Wang;Yun Wang;Weimin Zhang;Yakun Guo;Yue Shen;Linghuo Jiang;Qingyu Wu;Chong Zhang;Yizhi Cai;Junbiao Dai
- 通讯作者:Junbiao DaiJunbiao Dai
Perfluoroalkyl substances in water, sediment, and fish from a subtropical river of China: Environmental behaviors and potential risk
中国亚热带河流水体、沉积物和鱼类中的全氟烷基物质:环境行为和潜在风险
- DOI:10.1016/j.chemosphere.2021.13251310.1016/j.chemosphere.2021.132513
- 发表时间:20222022
- 期刊:
- 影响因子:8.8
- 作者:Siquan Wang;Yizhi Cai;Liya Ma;Xiaoping Lin;Qin Li;Yongyu Li;Xinhong WangSiquan Wang;Yizhi Cai;Liya Ma;Xiaoping Lin;Qin Li;Yongyu Li;Xinhong Wang
- 通讯作者:Xinhong WangXinhong Wang
Screening and characterization of aging regulators using synthesized yeast chromosome XIII
使用合成酵母 XIII 染色体筛选和表征衰老调节剂
- DOI:
- 发表时间:20232023
- 期刊:
- 影响因子:0
- 作者:Chun Zhou;Yun Wang;Yikun Huang;Yongpan An;Xian Fu;Daqian Yang;Yilin Wang;Jintao Zhang;Leslie A. Mitchell;J. Bader;Yizhi Cai;Junbiao Dai;J. Boeke;Zhiming Cai;Zhengwei Xie;Yue Shen;Weiren HuangChun Zhou;Yun Wang;Yikun Huang;Yongpan An;Xian Fu;Daqian Yang;Yilin Wang;Jintao Zhang;Leslie A. Mitchell;J. Bader;Yizhi Cai;Junbiao Dai;J. Boeke;Zhiming Cai;Zhengwei Xie;Yue Shen;Weiren Huang
- 通讯作者:Weiren HuangWeiren Huang
Towards Modeling Automation for Synthetic Biology
迈向合成生物学建模自动化
- DOI:
- 发表时间:20142014
- 期刊:
- 影响因子:0
- 作者:Chen Liao;Yizhi CaiChen Liao;Yizhi Cai
- 通讯作者:Yizhi CaiYizhi Cai
Methods to synthesize large DNA fragments for synthetic yeast genome
用于合成酵母基因组的大DNA片段的合成方法
- DOI:
- 发表时间:20172017
- 期刊:
- 影响因子:0
- 作者:Yizhi Cai;Junbiao DaiYizhi Cai;Junbiao Dai
- 通讯作者:Junbiao DaiJunbiao Dai
共 12 条
- 1
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Yizhi Cai的其他基金
GREAT: Genome Refactoring and Engineering Approach to study non-coding genes driving Translation
伟大:研究驱动翻译的非编码基因的基因组重构和工程方法
- 批准号:EP/Y024753/1EP/Y024753/1
- 财政年份:2024
- 资助金额:$ 10.12万$ 10.12万
- 项目类别:Research GrantResearch Grant
A UK-Japan partnership for synergising synthetic biology with systems biology.
英国-日本合作伙伴关系,旨在协同合成生物学与系统生物学。
- 批准号:BB/X018318/1BB/X018318/1
- 财政年份:2023
- 资助金额:$ 10.12万$ 10.12万
- 项目类别:Research GrantResearch Grant
UKRI Switzerland Partnering Awards for a UK-Swiss Engineering Biology Meeting
UKRI 瑞士英国-瑞士工程生物学会议合作奖
- 批准号:BB/X004937/1BB/X004937/1
- 财政年份:2023
- 资助金额:$ 10.12万$ 10.12万
- 项目类别:Research GrantResearch Grant
Engineering and safeguarding synthetic genomes
工程和保护合成基因组
- 批准号:EP/V05967X/1EP/V05967X/1
- 财政年份:2022
- 资助金额:$ 10.12万$ 10.12万
- 项目类别:FellowshipFellowship
21EBTA: Engineering Biology with Synthetic Genomes (EBSynerGy)
21EBTA:合成基因组工程生物学 (EBsynerGy)
- 批准号:BB/W014483/1BB/W014483/1
- 财政年份:2022
- 资助金额:$ 10.12万$ 10.12万
- 项目类别:Research GrantResearch Grant
Synthetic chromosomes to decipher requirements for optimal transmission of DNA in yeast
合成染色体破译酵母 DNA 最佳传输的要求
- 批准号:BB/S018301/1BB/S018301/1
- 财政年份:2019
- 资助金额:$ 10.12万$ 10.12万
- 项目类别:Research GrantResearch Grant
From genetic parts to neochromosome in yeast
从酵母的遗传部分到新染色体
- 批准号:BB/P02114X/1BB/P02114X/1
- 财政年份:2018
- 资助金额:$ 10.12万$ 10.12万
- 项目类别:Research GrantResearch Grant
An engineering platform for rapid prototyping synthetic genetic networks
用于快速构建合成遗传网络原型的工程平台
- 批准号:EP/P017401/1EP/P017401/1
- 财政年份:2017
- 资助金额:$ 10.12万$ 10.12万
- 项目类别:Research GrantResearch Grant
14-ERASynBio - IESY - Inducible Evolution of Synthetic Yeast genomes
14-ERASynBio - IESY - 合成酵母基因组的诱导进化
- 批准号:BB/M005690/1BB/M005690/1
- 财政年份:2015
- 资助金额:$ 10.12万$ 10.12万
- 项目类别:Research GrantResearch Grant
Building national hardware and software infrastructure for UK DNA Foundries
为英国 DNA 铸造厂建设国家硬件和软件基础设施
- 批准号:BB/M025640/1BB/M025640/1
- 财政年份:2015
- 资助金额:$ 10.12万$ 10.12万
- 项目类别:Research GrantResearch Grant
相似海外基金
14-ERASynBio - IESY - Inducible Evolution of Synthetic Yeast genomes
14-ERASynBio - IESY - 合成酵母基因组的诱导进化
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- 项目类别:Research GrantResearch Grant
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