CHRONIC FATIGUE IN LYME DISEASE

莱姆病引起的慢性疲劳

• 批准号: 3146583
• 负责人: PATRICIA K COYLE
• 金额: $ 24.3万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3146583
• 关键词: Borrelia; Lyme disease; antibacterial antibody; bacterial antigens; biomarker; blood chemistry; cerebrospinal fluid; chronic fatigue syndrome; cognition disorders; communicable disease diagnosis; cytokine; density gradient ultracentrifugation; disease /disorder proneness /risk; enzyme linked immunosorbent assay; human subject; humoral immunity; hypersensitivity; longitudinal human study; lymphocyte proliferation; major histocompatibility complex; medical records; pathologic process; psychological tests; statistics /biometry; western blottings

The objective is to define pathogenic mechanisms involved in chronic fatigue syndrome. Studies will concentrate on patients with chronic fatigue + cognitive impairment following infection by the spirochete B.burgdorferi (Bb) (the cause of Lyme disease). Controls will be matched Bb-infected subjects without such problems. Subjects will be evaluated at single time points and serially for clinical parameters (using fatigue and cognition rating scales) and immune parameters. The following specific points will be addressed: 1. Are predisposing factors involved? Document HLA-DR type, allergy history, depression/psychologic profile, and skin test response of patients vs. controls. 2. Is immune activation involved? Examine blood and cerebrospinal fluid for immune complexes (IC) using polyethylene glycol, chromatography, and Raji cell assay; spontaneous, mitogen, and antigen-induced lymphocyte proliferation; and cytokines/soluble markers using ELISA in patients vs. controls. 3. Is immune activation directed against Bb? Isolate IC to probe for anti-Bb antibodies. Compare in vitro lymphocyte reactivity and cytokine production to Bb in patients vs. controls. 4. Compare the immune profile of patient with fatigue alone vs cognitive impairment. Correlate humoral, cellular, and soluble immune parameters with measured clinical parameters of fatigue and cognition. 5. Do fatigue or cognitively impaired patients have persistent Bb infection? Probe isolated IC for Bb antigens using monoclonal/polyclonal antibodies, ELISA, and immunoblot. This study targets a specific subgroup of postinfectious fatigue patients to examine the role of immune activation and persistent infection in their fatigue, and the relationship of cognitive impairment to fatigue. This work should provide insights for the heterogeneous group of patients with chronic fatigue syndromes.

目的是定义涉及慢性的致病机制 疲劳综合征。 研究将集中于慢性患者 疲劳 +螺旋体感染后的认知障碍 B.Burgdorferi（BB）（莱姆病的原因）。 控件将匹配 没有此类问题的BB感染受试者。 主题将在 单个时间点和临床参数的串行（使用疲劳和 认知等级量表）和免疫参数。 以下特定 积分将被解决： 1。易于涉及的因素吗？ 文档HLA-DR类型，过敏 病史，抑郁/心理概况和患者的皮肤测试反应 与控件。 2。免疫激活涉及吗？ 检查血液和脑脊液 用于免疫复合物（IC），使用聚乙烯乙二醇，色谱和色谱和 拉吉细胞测定；自发，有丝分裂原和抗原诱导的淋巴细胞 增殖;在患者中使用ELISA使用ELISA的细胞因子/可溶性标记。 控件。 3。免疫激活针对BB？ 隔离IC至探测 抗BB抗体。 比较体外淋巴细胞反应性和细胞因子 在患者与对照组中生产BB。 4。比较单独疲劳与认知的患者的免疫特征 损害。 相关的体液，细胞和可溶性免疫参数 具有测量的疲劳和认知临床参数。 5。疲劳或认知受损的患者具有持久的BB 感染？ 使用单克隆/多克隆的探针分离BB抗原的IC 抗体，ELISA和免疫印迹。 这项研究针对感染后疲劳患者的特定亚组 检查免疫激活和持续感染在其中的作用 疲劳以及认知障碍与疲劳的关系。 这 工作应为异构患者组提供见解 慢性疲劳综合征。