Application of the SMALP system to generate antibodies for intact transmembrane proteins

应用 SMALP 系统生成完整跨膜蛋白抗体

• 批准号: BB/J010812/1
• 负责人: Michael Overduin
• 金额: $ 19.31万
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FJ010812%2F1
• 关键词: Application; SMALP; system; generate; antibodies; Application; SMALP; system; generate; antibodies

The analysis and exploitation of transmembrane proteins has recently become one of the most exciting areas of drug discovery and biological research. About a third of all proteins are found in membranes, their mechanisms are particularly difficult to study as they are often present only in small amounts and are difficult to handle. These proteins are stabilized by their interactions with native lipids but current methods to study them depend on detergents which strip these lipids away. We have developed a novel bionanoparticle to prepare functionally intact proteins directly from native membrane maintaining the local lipid environment and propose to develop antibodies to these targets for therapeutic and research purposes. This new generation of antibodies will allow specific recognition of native protein states with unprecedented accuracy, and will enable development of new diagnostic tests and biotherapeutic agents for treatment of cancer and many infectious and genetic diseases.

跨膜蛋白的分析和剥削最近已成为药物发现和生物学研究中最令人兴奋的领域之一。在膜中发现了所有蛋白质的三分之一，它们的机制尤其难以研究，因为它们通常仅以少量存在并且难以处理。这些蛋白质通过与天然脂质的相互作用而稳定，但是当前研究它们的方法取决于将这些脂质剥离的洗涤剂。我们已经开发了一种新型的bionanoparticle，可以直接从天然膜上制备功能完整的蛋白质，从而维持局部脂质环境，并建议开发针对这些靶标的抗体，以进行治疗和研究目的。这种新一代的抗体将允许以前所未有的准确性对天然蛋白质态进行特定识别，并能够开发新的诊断测试和生物治疗剂来治疗癌症以及许多传染性和遗传疾病。