Male germline regulatory mechanisms integrating cell cycle control with gamete specification.

雄性种系调节机制将细胞周期控制与配子规范相结合。

• 批准号: BB/I011269/1
• 负责人: David Twell
• 金额: $ 49.26万
• 依托单位: University of Leicester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FI011269%2F1
• 关键词: Male; germline; regulatory; mechanisms; integrating

Plant sexual reproduction is of vital importance both in the natural environment and for crop production. As in animals, plant reproduction relies on the production of male and female reproductive cells (sperms and eggs) that are united at fertilization. This research aims to uncover the underlying processes that control the production and fertility of plant sperm cells that are formed within pollen grains during sexual reproduction. Surprisingly, the molecular pathways that regulate sperm cell formation in flowering plants are largely unknown, even though this process is vital for plant breeding, crop and seed production. We have identified a master regulator gene, termed DUO1 that is vital for sperm cell formation and fertility in the model plant species Arabidopsis thaliana, a close relative of important seed and vegetable crops in the mustard family. The DUO1 gene has an important coordinating role in plant sperm cell development, controlling the activity of a wide network of genes involved in the control of cell division, and in the specialised function of sperm cells at fertilization. In the proposed work we will investigate for the first time how this network of regulatory factors controls plant sperm cell production using a range of technologies involving molecular, genetic and cell biological approaches. We will identify how different sets of genes are coordinated within this network to control cell division and the specialised production of plant sperm cells. This project will provide valuable information on how plants reproduce, allow meaningful comparisons to animal reproduction and generate new knowledge and technology that will allow greater understanding of this economically important part of the plant life cycle. Moreover, collaborative work with rice reproductive biology experts working in China has been established providing a direct route to evaluate the transfer of knowledge in an important food crop in which technologies such as hybrid seed production have significant (~20%) yield advantage. The work will be carried out by a full-time postdoctoral research assistant and a laboratory technician at the University of Leicester. Some aspects of the work will involve collaboration with researchers involving exchange of data and materials with Universities in China and Japan. Some of the specialised high throughput molecular work will be carried out using services provided by the VIB research institute, Belgium.

植物有性繁殖对于自然环境和作物生产都至关重要。与动物一样，植物的繁殖依赖于受精时结合的雄性和雌性生殖细胞（精子和卵子）的产生。这项研究旨在揭示控制有性生殖过程中花粉粒内形成的植物精子细胞的产生和受精的潜在过程。令人惊讶的是，在开花植物中调节精子细胞形成的分子途径在很大程度上是未知的，尽管这个过程对于植物育种、作物和种子生产至关重要。我们已经确定了一个名为 DUO1 的主调控基因，它对于模型植物拟南芥（芥菜科重要种子和蔬菜作物的近亲）的精子细胞形成和生育能力至关重要。 DUO1 基因在植物精子细胞发育中具有重要的协调作用，控制着涉及细胞分裂控制的广泛基因网络的活性，以及​​精子细胞在受精时的特殊功能。在拟议的工作中，我们将首次研究这一调控因子网络如何利用一系列涉及分子、遗传和细胞生物学方法的技术来控制植物精子细胞的产生。我们将确定不同的基因组如何在该网络内协调来控制细胞分裂和植物精子细胞的专门生产。该项目将提供有关植物如何繁殖的宝贵信息，与动物繁殖进行有意义的比较，并产生新的知识和技术，使人们更好地了解植物生命周期中这一经济上重要的部分。此外，与在中国工作的水稻繁殖生物学专家的合作已经建立，为评估重要粮食作物的知识转移提供了直接途径，其中杂交种子生产等技术具有显着（约20％）的产量优势。这项工作将由莱斯特大学的一名全职博士后研究助理和一名实验室技术员来完成。这项工作的某些方面将涉及与研究人员的合作，涉及与中国和日本的大学交换数据和材料。一些专门的高通量分子工作将利用比利时 VIB 研究所提供的服务进行。

项目成果

Genetic control of generative cell shape by DUO1 in Arabidopsis.

• DOI: 10.1007/s00497-023-00462-x
• 发表时间: 2023-09
• 期刊: Plant reproduction
• 影响因子: 3.4

Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants.

• DOI: 10.1007/978-1-4939-7286-9_22
• 发表时间: 2017
• 期刊: Methods in molecular biology
• 作者: Benjamin Peters;Jack Aidley;M. Cadzow;D. Twell;L. Brownfield

Overexpression of TWO-IN-ONE Domains Inhibits Cytokinesis in Arabidopsis

二合一结构域的过度表达抑制拟南芥中的细胞分裂

• DOI: 10.1007/s12374-022-09353-6
• 发表时间: 2022
• 期刊: Journal of Plant Biology
• 影响因子: 2.9
• 作者: Oh S

Epigenetic reprogramming rewires transcription during the alternation of generations in Arabidopsis.

• DOI: 10.7554/elife.61894
• 发表时间: 2021-01-25
• 期刊: eLife
• 影响因子: 7.7
• 作者: Borg M;Papareddy RK;Dombey R;Axelsson E;Nodine MD;Twell D;Berger F
• 通讯作者: Berger F