IMMUNOLOGICAL STUDIES WITH SYNTHETIC POLYPEPTIDES

合成多肽的免疫学研究

• 批准号: 3124391
• 负责人: PAUL H MAURER
• 金额: $ 15.68万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-01-01 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3124391
• 关键词: B lymphocyte; T lymphocyte; affinity chromatography; aminoacid; antibody titering; antigen antibody reaction; antigens; cellular immunity; delayed hypersensitivity; electrofocusing; gel electrophoresis; gene complementation; genes; genetic mapping; helper T lymphocyte; histocompatibility antigens; immune tolerance /unresponsiveness; immunization; immunochemistry; immunogenetics; immunoglobulins; immunoregulation; macrophage; oligopeptides; polymers; radiotracer; suppressor T lymphocyte; tissue /cell culture; ultracentrifugation

The research will continue to employ synthetic polymers of amino acids (T cell dependent and major histocompatibility-complex linked) to study aspects of genetic control of the immune response (lr) in mice. Wild mice (B10.W) having known la antigens, and H-2bm12 mice with mutations lA will be immunized with H-2b linked immunogens to determine the association of lr and known la antigens; i.e. lr=la? Anti-lD against the polymers GLPhe, GPhe, GAPhe, GLA40 and some sequential polymers will be studied to determine a) whether the anti polymer idiotypic determinants (VH region?) from mice within a murine strain as well as with different strains might be conserved and if so the extent of it and b) the possible importance of the association of the lD determinants in anti-GPhe with H2q. The biological activities of the various anti-lD as they effect both B cell i.e. PFC responses, and T cell functions i.e. proliferative responses: will be investigated. The anti-lD will be used to determine the nature of the ID determinants on suppressor, or helper T cells: or "factors" from responder and non-responder mice. The characterization of antigen specific T cell receptors (GLA40 system) will continue and will include determining the possible association of lD and la determinants with "the receptor." Our present success in the production of rabbit anti-lD sera against GLA40 and the production of hybridomas (monoclonal) against GLA40 are important for receptor work. In order to obtain significant amounts of T cells for isolation of receptors, we are developing techniques for T cell cloning and hybridization. The following systems of indicated H-2 will be studied to determine the number of unique T cell lines that may be produced and separated, and the antigen presenting cell haplotype specificities that may be involved. The l-GLPhe9:a) Fl(H-2f x H-2s) responding to GLPhe9 and GLT, b) Fl(H-2d x H-2s) responding to GLPhe9, GLT and GPhe, c) Fl(H-2d x H-2q) responding to GLPhe9, GLT and GPhe, d) H-2q responding to GLT and GPhe. The 2-GLT15: Fl(H-2f x H-2s) responding to GLT15 and GLPhe. The 3-GPhe: Fl(H-2d x H-2s). Sufficient amounts of antibody are being prepared against some of the sequential polymers so that anti-lD sera can be raised and the lD determinants characterized and compared within a single respnding haplotype congenic mice or from 2 different responding haplotypes.

该研究将继续采用氨基酸的合成聚合物（t 链接的细胞依赖性和主要的组织相容性复合） 小鼠免疫反应（LR）的遗传控制方面。 野鼠 （B10.W）已知LA抗原，而H-2BM12小鼠具有突变LA 用H-2B连接的免疫原豁免以确定LR的关联 和已知的La抗原；即lr = la？ 反向聚合物glphe的抗LLD， GPHE，Gaphe，Gla40和一些顺序聚合物将被研究到 确定a）抗聚合物白痴型的决定因素（VH区域？）是否是否？ 从鼠菌株中的小鼠以及不同的菌株中的小鼠可能是 保守的，如果是这样的程度，b）可能的重要性 抗GPHE中LD决定因素与H2Q的关联。 生物学 各种抗LLD的活性都会影响B细胞，即PFC 响应和T细胞功能，即增生响应：将是 调查。 反向将用于确定ID的性质 抑制器或辅助T细胞上的决定因素：响应者的“因子” 和无反应的小鼠。 抗原特异性T细胞的表征 受体（GLA40系统）将继续并包括确定 LD和LA决定因素与“受体”的可能关联。 我们的 目前在兔子抗LLD血清对GLA40和 针对GLA40的杂交瘤（单克隆）的产生对于 受体工作。 为了获得大量的T细胞 分离受体，我们正在开发用于T细胞克隆的技术和 杂交。将研究以下指示的H-2系统 确定可能产生的唯一T细胞系的数量，并 分离，抗原呈现细胞单倍型特异性可能 参与。 L-glphe9：a）FL（H-2F X H-2S）对GLPHE9和GLT的反应， b）FL（H-2D X H-2S）响应GLPHE9，GLT和GPHE，C）FL（H-2D X H-2Q） 响应GLPHE9，GLT和GPHE，D）H-2Q对GLT和GPHE响应。 2GLT15：FL（H-2F X H-2S）对GLT15和GLPHE响应。 3-gphe： FL（H-2D X H-2S）。 正在准备足够量的抗体 一些顺序聚合物，以便可以升高抗LLD血清，并 LD决定因素表征并在单个复位中进行了比较 单倍型先天小鼠或来自两种不同反应的单倍型。