NEURAL INFLUENCE ON AGING OF RECEPTOR CELLS

神经对受体细胞老化的影响

• 批准号: 3117626
• 负责人: STEPHEN D. ROPER
• 金额: $ 12.77万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-01-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3117626
• 关键词: Urodela; aging; autoradiography; cell age; cell growth regulation; chemoreceptors; denervation; electron microscopy; epithelium; familial dysautonomia; histochemistry /cytochemistry; mathematical model; microelectrodes; neurons; neurophysiology; radiotracer; scanning electron microscopy; single cell analysis; synapses; taste; taste threshold; tongue

The vertebrate taste bud is a good model for studying cellular aging in the nervous system. Taste cells possess many characteristics shared by neurons, such as excitability, chemosensitivity, and synapses (Roper, 1983a; Kinnamon, Taylor, Delay and Roper, 1985; Kinnamon and Roper, 1986). Taste cells have the added advantage that their lifespan is short (only a few days) compared to neurons, and thus age-related changes can be measured with reliability and confidence: complicating factors such as metabolic or hormonal differences between young and old animals are avoided. Furthermore, detailed analyses of age- related changes in the biophysical properties of taste cells and in their membrane chemosensitivity can be conducted on taste cells. Taste cells from the mudpuppy (Necturus maculosus) will be impaled with microelectrodes in situ, using a new isolated preparation developed in this laboratory (Roper, 1983). In depth analyses of the membrane conductances, particularly potassium conductance, will shed light on how ionic channels change over the lifespan of an excitable cell. Chemical stimuli will be applied focally onto the apical surface of taste cells to sutdy what age- related changes in chemosensitivity occur. Cells will be impaled with dye-filled micropipettes to identify the cells in subsequent light and electron micrographs in order to correlate the functional properites of the cell with its age: recent observations have indicated that taste cell aging can be determined by ultrastructural features (Kinnamon, et al. 1985; Delay, Kinnamon and Roper, 1986). These findings will provide information on cellular aging in excitable tissues and will also lead to a better understanding of taste transduction mechanisms.

脊椎动物的味蕾是研究细胞的良好模型 神经系统的老化。 味觉细胞拥有许多 神经元共有的特征，例如兴奋性、 化学敏感性和突触（Roper，1983a；Kinnamon，Taylor， 迪莱和罗珀，1985；金纳蒙和罗珀，1986）。 味觉细胞 还有一个优点是它们的寿命很短（只有少数 天）与神经元相比，因此与年龄相关的变化可以 可靠且有信心地进行测量：复杂的因素 例如年轻人和老年人之间的新陈代谢或荷尔蒙差异 动物被避开。 此外，还对年龄进行了详细分析 味觉细胞生物物理特性的相关变化 它们的膜化学敏感性可以在味觉细胞上进行。 来自泥巴犬（Necturus maculosus）的味觉细胞将 使用新的隔离微电极原位刺穿 该实验室开发了制剂（Roper，1983）。 深入 膜电导分析，特别是钾电导分析 电导，将揭示离子通道如何变化 可兴奋细胞的寿命。 将应用化学刺激 集中到味觉细胞的顶端表面来研究年龄- 化学敏感性发生相关变化。 细胞会被刺穿 用充满染料的微量移液器来识别随后的细胞 光学和电子显微照片，以便将功能关联起来 细胞的特性及其年龄：最近的观察 表明味觉细胞的老化可以通过以下方式确定 超微结构特征（Kinnamon 等人，1985 年；Delay，Kinnamon 和罗珀，1986）。 这些发现将提供有关细胞衰老的信息 兴奋组织，也将导致更好的理解 味觉传导机制。