PHOSPHORYLATED BOVINE PROLACTIN AND GROWTH HORMONE

磷酸化牛催乳素和生长激素

• 批准号: 3072607
• 负责人: CHARLES L BROOKS
• 金额: $ 6.44万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3072607
• 关键词: affinity chromatography; bioassay; biological response modifiers; caseins; circular dichroism; cow; disulfide bond; epididymis; hormone receptor; hormone regulation /control mechanism; laboratory mouse; laboratory rat; liver cells; lymphoma; mammary gland; peptide hormone metabolism; phosphoproteins; phosphorylation; pituitary gland; posttranslational modifications; prolactin; protein structure; protein structure function; radioimmunoassay; secretion; somatotropin; statistics /biometry; tissue /cell culture; affinity chromatography; bioassay; biological response modifiers; caseins; circular dichroism; cow; disulfide bond; epididymis; hormone receptor; hormone regulation /control mechanism; laboratory mouse; laboratory rat; liver cells; lymphoma; mammary gland; peptide hormone metabolism; phosphoproteins; phosphorylation; pituitary gland; posttranslational modifications; prolactin; protein structure; protein structure function; radioimmunoassay; secretion; somatotropin; statistics /biometry; tissue /cell culture

The structure of a hormone determines its function in target tissues. Recently, we have identified phosphorylated bovine prolactin (bPRL) and growth hormone (bGH). We have isolated and characterized the phosphorylated bPRL and have preliminary evidence that the structure of phosphorylated bPRL is different from nonphosphorylated bPRL. The hypothesis to be tested in this proposal will determine if in vivo posttranslational phosphorylation of either bPRL or bGH creates a hormone messenger with biological activities different from those of the native hormone. Posttranslational phosphorylation offers a mechanism by which the pituitary can specifically tailor several endocrine messages from a single gene product. These findings may have significant implications in our understanding of the physiology of the endocrine system. The bovine pituitary provides a system where isolation and characterization of PRL and GH are neither complicated by the structural and biological consequences of glycosylation nor limited by tissue availability. The specific objectives to test the hypothesis are: 1.Isolate and structurally characterize phosphorylated bPRL and bGH. 2. Determine the biological actions of phosphorylated bPRL and bGH in a series of bioassays and receptor assays and compare these activities with those induced by nonphosphorylated and reference preparations. If the hypothesis is correct then: 3.Determine the physiological agents that regulate the phosphorylation and secretion of bovine prolactin and growth hormone.

激素的结构决定了其在靶组织中的功能。 最近，我们确定了磷酸化的牛催乳素（BPRL）和 生长激素（BGH）。 我们已经孤立并表征了 磷酸化的BPRL并具有初步证据表明 磷酸化的BPRL与非磷酸化的BPRL不同。 该提议中要检验的假设将确定是否在 BPRL或BGH的体内翻译后磷酸化产生了 激素使者的生物学活动与 天然激素。 翻译后磷酸化提供了一种机制 垂体可以专门定制几条内分泌信息 来自单个基因产物。 这些发现可能具有重要的 我们对内分泌生理学的理解的含义 系统。 牛垂体提供了一个隔离和隔离的系统 PRL和GH的表征既不因结构而复杂 糖基化的生物学后果或受组织限制 可用性。 检验假设的特定目标是：1。隔离和 结构表征磷酸化的BPRL和BGH。 2。确定 一系列磷酸化BPRL和BGH的生物学作用 生物分析和受体分析，并将这些活动与这些活动进行比较 由非磷酸化和参考准备诱导。 如果假设正确，则：3。确定生理学 调节牛的磷酸化和分泌的药物 催乳素和生长激素。