CAPACITOR-BASED INTERFACE FOR MASS SPECTROMETRY

基于电容的质谱接口

• 批准号: 2678764
• 负责人: Murray Hackett
• 金额: $ 10.52万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2678764
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; dielectric property; electrospray ionization mass spectrometry; mass spectrometry

DESCRIPTION (Adapted from the applicant's abstract): The goal of this project is to develop an ultra sensitive liquid phase MS interface, based on the cylindrical capacitor ionization (CCI) technique which has recently been developed in the applicant's laboratory. The new CCI interface does not require an externally applied electric field around the spray tip, such as the metal needle and a counter electrode arrangement normally employed in electrospray ionization (ESI). The sample liquid is charged inside the capacitor instead of primarily at the spray tip. This charged liquid then can be sprayed into a space which may have a variety of pressure and electrical conditions present. The new design will limit corona discharge, and will allow one to put an ion focusing desolvation device near the spray tip, which can be below atmospheric pressure. This new design will have much better ion transmission efficiency relative to existing ESI sources. It will be less solvent dependent and more salt tolerant for most biological samples and will work equally well in positive and negative ion mode. Like electrospray, this new device will be applicable to many types of instruments, including quadrupole, quadrupole ion trap, Fourier transform, and time-of-flight MS. The signal/noise improvements observed for biomolecules are expected to be 10 to 500-fold. Preliminary data generated using a CCI front end, with an otherwise factory standard Finnigan heated capillary interface, indicated roughly a 500-fold improvement in concentration detection limits for the negative ion infusion analysis of diphosphorylated lipid A, at a flow rate of 50 nL/min. The greatest impact on biomedical research will be felt in the trace analysis of biomolecules that are difficult to spray, or that have relatively high detection limits, using existing electrospray technology. This would include many biological anions, or compounds that readily form anions in the gas phase, such as glycolipids, carbohydrates, nucleic acids, acidic proteins and acidic peptides. Improvements are also expected for the analysis of positive ions of biological origin.

描述（根据申请人的摘要改编）：目的 项目是基于 圆柱电容器电离（CCI）技术最近已经是 在申请人的实验室中开发。 新的CCI接口不 在喷雾尖端周围需要外部施加的电场，例如 通常在 电喷雾电离（ESI）。 样品液体在内部充电 电容器，而不是主要在喷雾尖端。 然后是充电的液体 可以喷洒到可能具有多种压力的空间中 电气条件存在。 新设计将限制电晕的排放， 并将允许将离子聚焦的脱溶设备在喷雾器附近放置 尖端，可能低于大气压力。 这个新设计将有 相对于现有的ESI源，离子传输效率更好。 对于大多数生物学而言，它的依赖性较小，耐盐性更高 样品并将在正离子模式下同样良好。 喜欢 电喷雾，该新设备将适用于多种类型的 仪器，包括四极，四极离子陷阱，傅立叶变换， 和飞行时间MS。 观察到的信号/噪声改善 预计生物分子为10至500倍。 生成的初步数据 使用CCI前端，带有其他工厂标准的Finnigan加热 毛细管界面，指示大约有500倍的改善 负离子输注分析的浓度检测极限 二磷酸化的脂质A，流速为50 nl/min。 最大的影响 生物医学研究将在生物分子的痕量分析中感受到 很难喷洒或具有相对较高的检测极限， 使用现有的电喷雾技术。 这将包括许多生物学 阴离子或在气相中易于形成阴离子的化合物，例如 糖脂，碳水化合物，核酸，酸性蛋白质和酸性 肽。 预计分析阳性离子也有所改进 生物来源。