LITHIUM INCREASES DOPAMINERGIC PHENOTYPE IN HNT NEURONS

锂增加 HNT 神经元的多巴胺能表型

• 批准号: 6054764
• 负责人: ALISON E WILLING
• 金额: $ 10万
• 依托单位: LAYTON BIOSCIENCE, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-04 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6054764
• 关键词: Parkinson's disease; cell transplantation; dopamine; dopamine receptor; lithium; method development; neurons; phenotype; serotonin; Parkinson's disease; cell transplantation; dopamine; dopamine receptor; lithium; method development; neurons; phenotype; serotonin

DESCRIPTION: (adapted from abstract): This is a first time Phase I application to investigate the stability and viability of hNT neurons exposed to lithium to increase DA-type phenotype. hNT neurons are postmitotic, terminally differentiated human neurons which can be used in transplant procedures for such diseases as Parkinson's. These neurons can express DA which make them a viable alternative to fetal tissue for such implants, however, the rate of DA expression is not high in these neurons. The rate can be increased significantly by exposure to LiCl. This proposal will determine whether the increased expression of TH is indicative of true functional DA phenotype and whether the phenotype continues to be expressed in an animal model of PD. PROPOSED COMMERCIAL APPLICATION: If this is shown to be the case then we will potentially have a highly dopaminergic cell that is available in large quantities that can replace fetal tissue for neural transplantation treatments in Parkinson's disease.

描述：（从摘要改编）：这是第一阶段的第一阶段 研究暴露于HNT神经元的稳定性和生存能力的应用 锂以增加DA型表型。 Hnt神经元是有丝分裂的，最终分化的人类神经元，可以 用于帕金森氏病等疾病的移植程序。这些 神经元可以表达DA，这使它们成为胎儿组织的可行替代品 但是，这些植入物在这些神经元中的DA表达速率不高。 通过暴露于LICL可以显着提高该利率。该提议将 确定增加Th的表达是否指示真实 功能性DA表型以及表型是否继续在 PD的动物模型。 拟议的商业应用： 如果证明是这种情况，那么我们可能会具有高度多巴胺能 大量可用的细胞可以替代胎儿组织的神经组织 帕金森氏病的移植治疗。