GENETIC ANALYSIS OF LEARNING PERFORMANCE

学习表现的遗传分析

• 批准号: 2191395
• 负责人: BRIAN H. SMITH
• 金额: $ 8.89万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2191395
• 关键词: Insecta; association learning; behavioral genetics; behavioral habituation /sensitization; discrimination learning; genetic markers; linkage mapping; male; molecular genetics; octopamine; olfactions; operant conditionings; performance; recombinant DNA; reversal learning; serotonin

Studies outlined below will investigate the quantitative and molecular genetic bases for variation in learning performance in the honey bee, Apis mellifera. Research into the bases of learning and memory in invertebrates such as mollusks and insects has demonstrated that fundamental properties of behavioral plasticity developed in studies of vertebrates apply across a broad phylogenetic spectrum. Yet, the genetic bases for learning traits are only beginning to be studied. Honey bees provide excellent opportunities for studying genetic factors that influence learning behavior because of the potential to apply several powerful behavioral paradigms for studying learning in vertebrates. The series of proposed studies will take advantage of the ability to train and analyze honey bee drones (males). Drones arise from unfertilized eggs and are thus haploid recombinants of the maternal genotype. Quantitative genetic selection on a haploid proceeds faster than it would on a diploid. Furthermore, linkage mapping from haploid genotypes using a map recently developed from a series of RAPD primers will not have the complications of such mapping using diploid genotypes. The specific aims of the studies are: * To further determine the quantitative genetic bases underlying variability in olfactory learning performance. Lines will be selected for fast versus slow reversal learning performance relative to unselected control lines. Use of this conditioning protocol and inclusion of appropriate control procedures will allow us to test whether associative learning performance can be selected without correlated responses in sensitization or motor systems. * To investigate the molecular genetic background using a linkage map established from RAPD genetic markers. These kinds of analyses will be carried out in recombinant F2 drone progeny of hybrid queens, in which spurious correlations due to founder effects in small base populations would be attenuated.

下面概述的研究将调查定量和分子 蜜蜂学习表现变异的遗传基础 梅利维拉。无脊椎动物学习和记忆基础的研究 例如软体动物和昆虫已经证明了基本特性 脊椎动物研究中发展的行为可塑性适用于 广泛的系统发育谱。然而，学习特征的遗传基础 才刚刚开始研究。 蜜蜂提供优良的 研究影响学习的遗传因素的机会 行为，因为有可能应用几种强大的行为 研究脊椎动物学习的范例。 提出的系列 研究将利用训练和分析蜜蜂的能力 雄蜂（雄性）。雄蜂由未受精卵产生，因此是单倍体 母本基因型的重组体。定量遗传选择 单倍体的进展速度比二倍体快。此外，联动 使用最近开发的图谱从单倍体基因型进行绘图 系列 RAPD 引物不会出现此类作图的并发症 使用二倍体基因型。研究的具体目标是： * 进一步确定潜在的定量遗传基础 嗅觉学习表现的可变性。 线路将被选择为 相对于未选择的快速与慢速逆转学习性能 控制线。 使用该调理方案并包含 适当的控制程序将使我们能够测试关联是否 可以在没有相关响应的情况下选择学习绩效 敏化或运动系统。 * 使用连锁图谱研究分子遗传背景 根据 RAPD 遗传标记建立。这些类型的分析将 在杂交蜂王的重组 F2 雄蜂后代中进行，其中 由于小基数人群的创始人效应而产生的虚假相关性 会被削弱。