POTASSIUM CHANNELS AND EPILEPTOGENESIS

钾通道与癫痫发生

• 批准号: 2735506
• 负责人: Jong Min Rho
• 金额: $ 10.56万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-23 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2735506
• 关键词: aging; brain disorder chemotherapy; developmental genetics; developmental neurobiology; electroencephalography; epilepsy; genetically modified animals; hippocampus; kainate; laboratory mouse; neural plasticity; neurogenetics; neurotoxicology; nonhuman therapy evaluation; phenobarbital; potassium channel; protein structure function; voltage /patch clamp

This application is for a Mentored Clinical Scientist Development Award (K08). The candidate obtained clinical training in Child Neurology at UCLA, and then completed a two-year fellowship in neuropharmacology at the National Institutes of Health (Epilepsy Research Branch, NINDS) prior to taking his current faculty appointment in 1994 at the University of Washington. The long- term career goal of the candidate is to study the relationship between ion channels and epileptogenesis, to identify potentially novel treatments for developmental epilepsies that have a rational molecular basis, and to explore the relationship between genetic susceptibility and environmental influence in the pathogenesis of the developmental epilepsies. The university medical center and Children's Hospital possess complementary strengths in the basic neurosciences and clinical pediatric epileptology, respectively. Laboratory space, equipment, and specialized consultants are available to the candidate on a collaborative basis provided by several academic departments. This environment is ideally suited for supporting and fostering the candidate's long-term professional goals. We have chosen as our experimental model the mKv1.1 potassiumchannel single gene mouse mutant which exhibits spontaneous seizures early in development. First, we hypothesize that loss of a specific potassium channel subunit in the CA 3 region of the hippocampus results in a functional alteration in physiological properties of pyramidal neurons such that they became hyperexcitable and prone to excessive synchronization of discharge. Second, we postulate that pharmacological treatment can alter the natural course of the epilepsy and may protect the animal from neuronal injury as a consequence of repeated seizures. Finally, we will explore whether partial expression of the mKv1.1 gene in plus/minus mice, resulting in an enhanced susceptibility to seizures, can be modified by secondary exposure to kainic acid (a potent convulsant and neurotoxin) early in development. Initial efforts will involve electrophysiological recordings from CA3 pyramidal neurons in hippocampal slices taken from null mutants at different ages; intrinsic and synaptic properties of these cells, as well as whole-cell potassium currents, will be measured with extracellular, intracellular and patch-clamp recording techniques. Video-EEG monitoring (with depth electrodes) will be employed to document epileptiform or seizure activity in the hippocampus, and histochemical analysis of neuronal damage and/or plasticity will be made with routine and special (e.g., Timm) stains. The results of these studies may lead to an improved understanding of the role ion channels play in epileptogenesis, may shed light on the controversial issue of whether chronic seizures can cause brain damage, and will provide a framework for further studies examining genetic and environmental interactions in the developing brain.

该应用程序适用于指导临床科学家的发展 奖（K08）。候选人接受了儿童临床培训 加州大学洛杉矶分校神经病学，然后完成了两年的研究金 美国国立卫生研究院神经药理学（癫痫 研究部门，NINDS）在担任现任教师之前 1994年受聘于华盛顿大学。 长- 候选人的职业目标是研究关系 离子通道和癫痫发生之间的关系，以识别潜在的 具有合理合理性的发育性癫痫新疗法 分子基础，探讨遗传之间的关系 发病机制中的易感性和环境影响 发育性癫痫。 大学医疗中心和 儿童医院基础优势互补 分别是神经科学和临床儿科癫痫学。 实验室空间、设备和专业顾问 在协作的基础上提供给候选人 几个学术部门。 这个环境非常适合 支持和培养候选人的长期专业 目标。 我们选择 mKv1.1 作为我们的实验模型 钾通道单基因小鼠突变体表现出 发育早期自发性癫痫发作。 首先，我们假设 CA 3 中特定钾通道亚基的丢失 海马体区域导致功能改变 锥体神经元的生理特性使得它们 变得过度兴奋并容易过度同步 释放。 其次，我们假设药物治疗 可以改变癫痫的自然病程并可以保护 动物因反复的神经元损伤 癫痫发作。 最后，我们将探讨是否部分表达 正/负小鼠中的 mKv1.1 基因，导致增强 对癫痫发作的易感性，可以通过二次暴露来改变 红藻氨酸（一种强效惊厥剂和神经毒素） 发展。 最初的努力将涉及电生理学 海马切片 CA3 锥体神经元的记录 取自不同年龄的无效突变体；内在和突触 这些细胞的特性以及全细胞钾电流， 将使用细胞外、细胞内和膜片钳进行测量 录音技术。视频脑电图监测（深度 电极）将用于记录癫痫样或癫痫发作 海马体活动和组织化学分析 神经元损伤和/或可塑性将通过常规和 特殊（例如 Timm）污渍。 这些研究的结果可能会导致 更好地了解离子通道在 癫痫发生，可能有助于解决这个有争议的问题 慢性癫痫发作是否会导致脑损伤，并会提供 进一步研究遗传和 发育中的大脑中的环境相互作用。