PRESYNAPTIC GLIAL ENHANCEMENT OF SYNAPTIC EFFICACY

突触前胶质细胞增强突触功效

• 批准号: 2777649
• 负责人: Erik M Ullian
• 金额: $ 2.5万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2777649
• 关键词: astrocytes; calcium channel; glia; laboratory rat; membrane potentials; nerve /myelin protein; neuroregulation; neurotransmitter transport; retinal ganglion; synapses; tissue /cell culture

Synapses throughout the human brain are closely associated with glia. Yet, the role of glia in the development and maintenance of synapses has not been thoroughly investigated. Preliminary data from Dr. Barres' laboratory indicate that synapses formed in the absence of glia in vitro have low efficacy. Glial cells can strongly enhance the efficacy of these synapses. We propose to further characterize how glial cells enhance synaptic efficacy. In particular, we will test the hypothesis that glia enhance synaptic efficacy by increasing the probability of presynaptic transmitter release. We will investigate how glial cells regulate synaptic efficacy. a) Do glial cells regulate transmitter synthesis? b) Do glial cells regulate transmitter release? c) Is electrical activity required for the glial enhancement of synaptic efficacy? d) Do glial cells enhance presynaptic calcium currents? e) Do glial cells upregulate expression of one or more presynaptic proteins.

整个人脑的突触与神经胶质细胞密切相关。 然而，神经胶质细胞在突触的发育和维持中的作用已经 没有被彻底调查。 巴雷斯博士的初步数据 实验室表明突触在体外没有神经胶质细胞的情况下形成 功效较低。 胶质细胞可以强烈增强 这些突触。 我们建议进一步表征神经胶质细胞如何 增强突触功效。特别是，我们将检验假设 神经胶质细胞通过增加突触的概率来增强突触功效 突触前递质释放。 我们将研究神经胶质细胞如何 调节突触功效。 a) 神经胶质细胞调节递质吗 合成？ b) 神经胶质细胞调节递质释放吗？ c) 是 神经胶质增强突触所需的电活动 功效？ d) 神经胶质细胞是否增强突触前钙电流？ e) 做 神经胶质细胞上调一种或多种突触前蛋白的表达。