BIOCHEMICAL STUDIES ON MHCK A RELATED KINASES

MHCK相关激酶的生化研究

• 批准号: 2465615
• 负责人: THOMAS EGELHOFF
• 金额: $ 23.45万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2465615
• 关键词: Dictyostelium; catalyst; cell biology; cell motility; chemotaxis; enzyme mechanism; enzyme structure; enzyme substrate; gene expression; gene mutation; microorganism genetics; myosins; phosphorylation; protein kinase; protein localization; protein sequence; protein structure function; tissue /cell culture; Dictyostelium; catalyst; cell biology; cell motility; chemotaxis; enzyme mechanism; enzyme structure; enzyme substrate; gene expression; gene mutation; microorganism genetics; myosins; phosphorylation; protein kinase; protein localization; protein sequence; protein structure function; tissue /cell culture

DESCRIPTION: The long-term goal of the proposed studies is to understand the biochemical properties and cellular regulation of protein kinases that control myosin localization in Dictyostelium. The goal of the proposed studies is to describe the fundamental biochemical properties and structure of MHCK-A and the related MHCK B. Four specific aims are proposed: (1) To determine which residues and regions of MHCK A are involved in catalytic function and substrate binding; (2) to determine the biochemical and physiological substrate specificity of MHCK B; (3) to determine if there are conserved autoinhibitory or activating motifs in different members of the MHCK family of kinases; and (4) to determine the roles of the WD-repeat domain in MHCK A.

描述：拟议研究的长期目标是了解 蛋白激酶的生化特性和细胞调节 控制肌球蛋白在Dictyostelium中的定位。 提议的目标 研究是为了描述基本的生化特性和结构 MHCK-A和相关的MHCKB。提出了四个具体目的：（1）到 确定MHCK A的残基和区域参与催化 功能和底物结合； （2）确定生化和 MHCK B的生理底物特异性； （3）确定是否有 保守的自身抑制或激活基序 MHCK激酶家族； （4）确定WD重复的作用 MHCK A.