NEURONAL ORGANIZATION IN SIMPLE NERVOUS SYSTEMS

简单神经系统中的神经组织

• 批准号: 2519953
• 负责人: Jian-Young Wu
• 金额: $ 10.67万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2519953
• 关键词: Aplysia; Mollusca; alternatives to animals in research; aquatic organism; behavior; behavioral /social science research tag; biological signal transduction; evoked potentials; fluorescent dye /probe; image processing; interneurons; neural information processing; neurobiology; neurons; paraganglia

The long term objective of this proposal is to understand the neuronal organization that underlies the generation of behaviors by simple nervous systems. To accomplish this goal, we will use voltage sensitive dyes imaging techniques to monitor a large fraction of neurons when behaviors are generated. We plan to study two molluscan ganglia, the Aplysia abdominal ganglion and the Clione intestinal ganglion. We have found that about 300 neurons are activated in the Aplysia abdominal ganglion during two kinds of gill withdrawal behaviors and about 70 per cent of the active neurons are activated in both behaviors. This suggests there may be a large distributed neuronal network in this ganglion. For Aplysia, we plan to monitor a large fraction of neurons during normal and altered behaviors. There are four specific aims: I. To test three models for possible neuronal organization suggested by our preliminary data. II. To examine how neurons are shared by different behavioral events III. To examine the interaction between evoked behavior and intrinsic rhythms and between two behaviors initiated close in time. IV. To study how system changes when the activity of individual neurons is modified and to find important interneurons in the ganglion. With these aims we will try to understand the neuronal organization in this ganglion and the role of individual neurons in a large and extensively inter- connected neuronal network. We will also examine the dynamic behavior of this simpler nervous system and explore the hypothetical "temporary circuits" which forms only during specific behaviors. We also plan to study a smaller nervous system-- the Clione intestinal ganglion. This ganglion has been evaluated in our preliminary experiment and may be unique for optically monitoring all the neurons during behaviors. Two specific aims are proposed on this new preparation: I. To identify all the neurons in the ganglion by combining voltage sensitive dye imaging and phase contrast enhancement. Neurons will be identified by both morphological features and the activity patterns during different behaviors. II. To study how neuronal activity changes when the neuronal hardware varies. We have found that there are a large variations in number of neurons in this ganglion. We will try to examine how neuronal activity patterns change when neurons are missing or there are extra neurons.

该提议的长期目标是了解神经元 简单紧张的组织是基础的行为的基础 系统。为了实现这一目标，我们将使用电压敏感染料 当行为时，成像技术可监测大量的神经元 生成。我们计划研究两个软体动物神经节，Aplysia 腹神经节和肠神经节。 我们发现大约300个神经元在垂直溶质中被激活 腹部神经节在两种g拔出行为中 在两种行为中，有70％的活性神经元被激活。这 建议在此中可能有大型分布式神经元网络 神经节。 对于Aplysia，我们计划在正常情况下监测大量的神经元 和行为改变。有四个特定的目标：I。测试三个 我们的初步提出的可能神经元组织的模型 数据。 ii。检查神经元如何通过不同的行为共享 事件III。检查诱发行为与 固有的节奏和两种行为之间的启动。 iv。 研究单个神经元的活动是如何变化的 修改并在神经节中找到重要的中间神经元。 与这些 目的我们将尝试了解这个神经节中的神经元组织 单个神经元在大型和广泛的相互之间的作用 连接的神经元网络。我们还将检查的动态行为 这种简单的神经系统并探讨了假设的“暂时性 电路”仅在特定行为时形成。 我们还计划研究一个较小的神经系统 - Clione肠道 神经节。该神经节已在我们的初步实验中进行了评估 并且在光学监测所有神经元期间可能是唯一的 行为。在这项新准备工作中提出了两个具体目标：I。 通过组合电压敏感来识别神经节中的所有神经元 染料成像和相对比增强。神经元将由 形态学特征和不同的活动模式 行为。 ii。研究神经元时的神经元活性如何变化 硬件有所不同。我们发现数字有很大的变化 在这个神经节中的神经元。我们将尝试研究神经元活动 当神经元缺失或额外的神经元时，模式会发生变化。