NEURONAL ORGANIZATION IN SIMPLE NERVOUS SYSTEMS

简单神经系统中的神经组织

• 批准号: 2519953
• 负责人: Jian-Young Wu
• 金额: $ 10.67万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2519953
• 关键词: Aplysia; Mollusca; alternatives to animals in research; aquatic organism; behavior; behavioral /social science research tag; biological signal transduction; evoked potentials; fluorescent dye /probe; image processing; interneurons; neural information processing; neurobiology; neurons; paraganglia

The long term objective of this proposal is to understand the neuronal organization that underlies the generation of behaviors by simple nervous systems. To accomplish this goal, we will use voltage sensitive dyes imaging techniques to monitor a large fraction of neurons when behaviors are generated. We plan to study two molluscan ganglia, the Aplysia abdominal ganglion and the Clione intestinal ganglion. We have found that about 300 neurons are activated in the Aplysia abdominal ganglion during two kinds of gill withdrawal behaviors and about 70 per cent of the active neurons are activated in both behaviors. This suggests there may be a large distributed neuronal network in this ganglion. For Aplysia, we plan to monitor a large fraction of neurons during normal and altered behaviors. There are four specific aims: I. To test three models for possible neuronal organization suggested by our preliminary data. II. To examine how neurons are shared by different behavioral events III. To examine the interaction between evoked behavior and intrinsic rhythms and between two behaviors initiated close in time. IV. To study how system changes when the activity of individual neurons is modified and to find important interneurons in the ganglion. With these aims we will try to understand the neuronal organization in this ganglion and the role of individual neurons in a large and extensively inter- connected neuronal network. We will also examine the dynamic behavior of this simpler nervous system and explore the hypothetical "temporary circuits" which forms only during specific behaviors. We also plan to study a smaller nervous system-- the Clione intestinal ganglion. This ganglion has been evaluated in our preliminary experiment and may be unique for optically monitoring all the neurons during behaviors. Two specific aims are proposed on this new preparation: I. To identify all the neurons in the ganglion by combining voltage sensitive dye imaging and phase contrast enhancement. Neurons will be identified by both morphological features and the activity patterns during different behaviors. II. To study how neuronal activity changes when the neuronal hardware varies. We have found that there are a large variations in number of neurons in this ganglion. We will try to examine how neuronal activity patterns change when neurons are missing or there are extra neurons.

该提案的长期目标是了解神经元 构成简单神经行为产生基础的组织 系统。为了实现这一目标，我们将使用电压敏感染料 成像技术可以监测大部分神经元的行为 被生成。我们计划研究两个软体动物神经节，海兔 腹神经节和肠肠神经节。 我们发现海兔体内大约有300个神经元被激活 腹神经节在两种退鳃行为过程中的变化及其影响 70% 的活跃神经元在这两种行为中都被激活。这 表明这可能存在一个大型的分布式神经元网络 神经节。 对于海兔，我们计划在正常情况下监测大部分神经元 并改变了行为。具体目标有四个： 一、测试三个 我们初步建议的可能的神经元组织模型 数据。 二.研究不同行为的神经元如何共享 事件三.检查诱发行为和 内在节奏以及两种行为之间的启动时间相近。 四． 研究当单个神经元的活动受到影响时系统如何变化 修改并找到神经节中重要的中间神经元。 有了这些 目标我们将尝试了解该神经节的神经元组织 以及单个神经元在大而广泛的相互作用中的作用 连接的神经元网络。我们还将检查的动态行为 这个更简单的神经系统并探索假设的“临时” 电路”仅在特定行为期间形成。 我们还计划研究一个更小的神经系统——Clone肠道 神经节。该神经节已在我们的初步实验中进行了评估 并且对于在光学监测过程中所有神经元来说可能是独一无二的 行为。这一新的准备工作提出了两个具体目标： 通过结合电压敏感识别神经节中的所有神经元 染料成像和相差增强。神经元将被识别为 不同时期的形态特征和活动模式 行为。二.研究当神经元受到干扰时，神经元活动如何变化 硬件各不相同。我们发现数量上存在很大差异 这个神经节中的神经元。我们将尝试检查神经元活动如何 当神经元缺失或有额外神经元时，模式就会发生变化。