DEVELOPMENT OF MUTAGENESIS ASSAY USING TRANSGENIC MICE

使用转基因小鼠进行诱变测定的开发

• 批准号: 3642318
• 负责人: CARL PINKERT
• 金额: $ 40.8万
• 依托单位: DNX, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-06-01 至 1994-05-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3642318
• 关键词: genetically modified animals; laboratory mouse; mutagen testing

The objectives of this project is to develop one or more in vivo mutagenesis assay systems to detect and quantitate gene mutations at a precisely defined target sequence in somatic, and possibly germ cells, of mice exposed to a test chemical. Such assays will provide a means to detect and analyze organ and tissue specific mutagenesis and study associations with chemical disposition, toxicity, and carcinogenicity. Ultimately, such systems may provide an in vivo short term assay with predictive value for carcinogenicity.

该项目的目标是开发一个或多个体内 诱变测定系统以检测和定量A处的基因突变 精确定义的在体细胞和可能生殖细胞中的靶序序 暴露于测试化学物质的小鼠。 这样的测定将为 检测和分析器官和组织特异性诱变并进行研究 与化学处置，毒性和致癌性的关联。 最终，这样的系统可以提供体内短期测定法 致癌性的预测价值。