VIBRATIONAL SPECTROSCOPY OF PROTEIN NUCLEATION AND GROWTH

蛋白质成核和生长的振动光谱

• 批准号: 3755800
• 负责人: STEVEN H MORGAN
• 金额: --
• 依托单位: FISK UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3755800
• 关键词: Raman spectrometry; X ray crystallography; aminoacid; analytical method; chemical bond resonance; chemical structure function; crystallization; infrared spectrometry; macromolecule; proteins; solubility; temperature; water solution

The inability to routinely grow single crystals of sufficient size and quality is a major roadblock to the wider application of protein crystallography. The development of more effective growth techniques requires a better understanding of the mechanism of protein crystallization. We proposed to apply some of the spectroscopic techniques used to investigate small molecule nucleation and growth to the study of the growth and nucleation of biological macromolecules Methodology will be developed using small molecule systems on which Raman studies of nucleation have been previously published. The applicability of infrared spectroscopy (GTIR-ATR) for detecting and identifying subnuclei at the very early stages of formation will also be determined. These techniques will be applied initially to amino acids, and then to peptides and proteins. Solubility data and super-saturation for proteins in H2O as a function of temperature, concentration of precipitating agent and pH will be obtained for systems studied. The use of temperature as a control parameter in protein crystal growth will also be investigated. Proteins suitable for temperature programmed growth experiments will be selected based upon either published solubility data or data obtained during the course of the proposed research.

无法常规生长足够尺寸和的单晶 质量是更广泛应用蛋白质的主要障碍 晶体学。 开发更有效的增长技术 需要更好地了解蛋白质的机制 结晶。 我们建议应用一些光谱技术 用于研究小分子成核和生长的研究 生物大分子方法的生长和成核方法将是 使用小分子系统开发的拉曼研究成核研究 以前已发表。 红外光谱的适用性 （GTIR-ATR）用于在很早的阶段检测和识别亚核 也将确定形成。 这些技术将被应用 最初是氨基酸，然后到肽和蛋白质。 溶解度 H2O中蛋白质的数据和超饱和度与温度的关系 对于系统，将获得沉淀剂和pH的浓度 研究。 将温度用作蛋白质晶体中的控制参数 增长也将被研究。 适合温度的蛋白质 将根据已发布的任何一个计划选择程序增长实验 在提议过程中获得的溶解度数据或数据 研究。