FEVER RESPONSE--PHYSIOLOGICAL/ECOLOGICAL ASPECTS

发烧反应——生理/生态方面

• 批准号: 3755836
• 负责人: ALAN E. MUCHLINSKI
• 金额: --
• 依托单位: CALIFORNIA STATE UNIVERSITY LOS ANGELES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3755836
• 关键词: Chelonia; alternatives to animals in research; animal ecology; epizootiology; field study; hyperthermia; lizards; snakes; squirrel; stimulus /response; telemetry

The research described in this proposal seeks to increase our knowledge concerning the fever response in animals other than humans; specifically relating to (1) the generality of the fever response in reptiles, (2) if there is a dose-response relationship between the level of bacteria infection in a reptile and the magnitude and duration of the fever response, (3) the relationship of the acute phase response to the increase in body temperature (which typically defines a fever), and (4) the occurrence of the fever response in a species of mammal (the California ground squirrel, Spermophilus beecheyi) living in its natural environment. Fever responses have been demonstrated in 7 lizards species, 3 snakes species, and 2 turtles species. However, a number of other species of reptiles have not exhibited a fever response upon injection with putative pyrogenic agents. We plan to test more species of reptiles to better define the parameters under which a fever will be demonstrated. A second set of experiments will explore possible dose-response relationships regarding the magnitude and duration of the fever response. Experiments reported in the literature have generally used a single dose of either live or dead bacteria in an attempt to demonstrate a fever response. We believe that more information can be obtained if more detailed studies use a wide range of bacteria dosages. A third set of experiments will better define the relationship between the level of bacteria infection, the presence or absence of the fever response as defined by a significant increase in body temperature, and certain aspects of the acute phase response. Specifically we will look at the aspects of the acute phase response that involve decreases in serum iron and zinc levels and increases in circulating white blood cell counts. A fourth set of experiments will study the response of a free-living mammal to bacteria infection. The physiological response of an animal maintained under laboratory conditions is not always the same as under natural conditions. Therefore, data gathered on the fever response in the natural environment is important for a better understanding of the fever phenomenon. Students will be involved in all aspects of this research project including data design, collection, and analysis, utilization of various pieces of scientific equipment, writing of scientific papers, and presentation of results at scientific meetings.

该提案中描述的研究旨在增加我们的知识 关于人类以外的动物的发烧反应；具体来说 与（1）爬行动物中发烧反应的一般性有关，（2）如果 细菌水平之间存在剂量反应关系 爬行动物感染以及发烧的幅度和持续时间 响应，（3）急性相响应与增加的关系 在体温（通常定义发烧）中，（4） 在哺乳动物（加利福尼亚州）中发生发烧反应 地松鼠，精子beecheyi）生活在其自然环境中。 发烧反应已在7种蜥蜴物种中证明了3条蛇 物种和2种海龟。 但是，许多其他物种 爬行动物在注射时没有表现出发烧反应 热原剂。 我们计划测试更多的爬行动物以更好 定义将显示发烧的参数。 第二 一组实验将探索可能的剂量反应关系 关于发烧反应的大小和持续时间。 实验 文献中报告通常使用了一剂 或死细菌试图证明发烧反应。 我们相信 如果更详细的研究使用广泛的研究，可以获得更多信息 细菌剂量范围。 第三组实验将更好地定义 细菌感染水平，发烧反应的存在或不存在 由体温显着升高和确定 急性相响应的各个方面。 具体来说，我们将查看 涉及血清铁降低的急性相响应的各个方面 和锌水平以及循环白细胞计数的增加。 一个 第四组实验将研究自由哺乳动物的反应 感染细菌。 维持动物的生理反应 在实验室条件下并不总是与自然相同 状况。 因此，收集关于自然发烧反应的数据 环境对于更好地理解发烧很重要 现象。 学生将参与该研究项目的各个方面 数据设计，收集和分析，利用各种 科学设备，科学论文的撰写和介绍 在科学会议上的结果。