RNA TRANSPORT IN DENDRITES

树突中的 RNA 运输

• 批准号: 2273295
• 负责人: HENRI TIEDGE
• 金额: $ 15.52万
• 依托单位: SUNY DOWNSTATE MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-01 至 1997-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2273295
• 关键词: Chordata; RNA; antimitotics; biological transport; biomarker; cytoskeleton; dendrites; electrophysiology; inhibitor /antagonist; intracellular transport; neural inhibition; neural plasticity; neural transmission; neuropharmacologic agent; neuropharmacology; neurotransmitters; nucleic acid sequence; nucleic acid structure; protein biosynthesis; radiotracer; tissue /cell culture

The phenomenon of dendritic protein synthesis in neurons has been well documented. The specific mRNAs for several proteins with specific dendritic functions or localizations are thought to be targeted to sites of active translation in dendrites. This protein synthesis is associated with the postsynaptic side of synaptic contacts and may provide a mechanism for modulation of postsynaptic functional plasticity. However, the mechanisms for specific transport and targeting of RNA, to dendrites have not been delineated. This application will examine the specific structural elements responsible for localization of BC1, a short, neuron- specific RNA, to dendrites. The specific sequence elements required for targeting to dendrites will be determined and their ability to target foreign chimeric RNA sequences to the dendrite will be evaluated. In the second series of experiments, the role of electrical activity and synaptic transmission on the expression and transport of RNA into dendrites will be assessed. The effects of inhibitors for synaptic transmission, electrical activity, and cytoskeletal organization on various components of the translational apparatus will be examined. It is hoped that these studies will provide new insights into the role of dendritic protein synthesis in neuronal plasticity.

神经元中树突状蛋白质合成的现象一直很好 记录。具有特定蛋白质的特定mRNA 树突状功能或定位被认为针对地点 树突中的主动翻译。该蛋白质合成是相关的 突触接触的突触后一侧，可能会提供 调节突触后功能可塑性的机制。然而， RNA的特定运输和靶向的机制，向树突 尚未被描述。该应用程序将检查特定 负责BC1定位的结构元素，一个简短的神经元 - 特定的RNA，至树突。需要的特定序列元素 将确定针对树突的目标，并将其靶向靶向 将评估外国嵌合RNA序列到树突的序列。在 第二系列实验，电活动的作用和 RNA表达和传输到达的突触传播 将评估树突。抑制剂对突触的影响 传播，电活动和细胞骨架组织 将检查转化设备的各种组成部分。它 希望这些研究能为您的作用提供新的见解 神经元可塑性中的树突状蛋白质合成。