CNS DERIVED TROPHIC AGENTS

中枢神经系统衍生的营养剂

• 批准号: 2269313
• 负责人: RONALD W OPPENHEIM
• 金额: $ 17.36万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-03-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2269313
• 关键词: brain; cell death; central nervous system; chick embryo; chromatography; denervation; developmental neurobiology; electrophoresis; fibroblast growth factor; gene expression; growth factor receptors; in situ hybridization; motor neurons; neural degeneration; neurotrophic factors; protein purification; spinal cord; spinal nerves

The long term goal of the proposed research is to identify, and to characterize biochemically and biologically, putative CNS-derived neurotrophic agents that influence the survival and development of spinal motoneurons (MNS) in the chick embryo. By capitalizing on our prior work indicating that a protein(s) derived from chicken CNS can promote the in vitro and in vivo survival on MNS, we will attempt to isolate and identify this agent(s) using standard methods of protein chemistry. We will also examine the in vitro and in vivo effects of members of the NGF or neurotrophin family on MN survival (e.g., NGF, BDNF, NT-3, and possibly NT-4 and NT-5). Finally, we will examine the in vivo expression of mRNAs for suspected trophic agents and their receptors in the avian CNS using in situ hybridization methodology. These include: NGF, FGF, CNTF, S100, BDNF and NT-3. The major significance of these studies is that they represent the first attempt to demonstrate that putative CNS-derived neurotrophic agents can act in vitro and in vivo to promote the survival of a population of neurons (MNS) that are at risk in human motor neuron diseases.

拟议研究的长期目标是识别和 在生物化学和生物学上，推定的CNS衍生 影响脊柱存活和发育的神经营养剂 小鸡胚胎中的运动神经元（MNS）。 通过利用我们的先前工作 表明源自鸡CNS的蛋白质可以促进In 体外和体内生存在MNS上，我们将尝试分离和 使用蛋白质化学的标准方法识别该药物。 我们 还将检查NGF成员的体外和体内影响 或神经营养蛋白家族在MN生存中（例如NGF，BDNF，NT-3和 可能是NT-4和NT-5）。 最后，我们将检查体内表达 可疑的营养剂及其受体中的mRNA 中枢神经系统使用原位杂交方法。 其中包括：NGF，FGF， CNTF，S100，BDNF和NT-3。 这些研究的主要意义是 他们代表了证明该假定的首次尝试 CNS衍生的神经营养剂可以在体外和体内作用以促进 在人类中处于危险中的神经元人群（MN）的生存 运动神经元疾病。

项目成果

The development of interneurons in the chick embryo spinal cord following in vivo treatment with retinoic acid.

视黄酸体内处理后鸡胚脊髓中中间神经元的发育。

• DOI: 10.1002/cne.903600308
• 发表时间: 1995
• 期刊: The Journal of comparative neurology.
• 作者: Shiga,T;Gaur,VP;Yamaguchi,K;Oppenheim,RW
• 通讯作者: Oppenheim,RW

Expression of apogens and engulfens during programmed cell death in the nervous system of the chick embryo.

鸡胚神经系统程序性细胞死亡过程中 apogen 和 engulfen 的表达。

• DOI: 10.1679/aohc.58.243
• 发表时间: 1995
• 期刊: Archives of histology and cytology
• 作者: Takashita,N;Homma,S;Rottello,RJ;Fernandez,PA;Yuan,J;Oppenheim,RW;Yaginuma,H
• 通讯作者: Yaginuma,H

Cell death of spinal motoneurons in the chick embryo following deafferentation: rescue effects of tissue extracts, soluble proteins, and neurotrophic agents.

鸡胚传入神经阻滞后脊髓运动神经元的细胞死亡：组织提取物、可溶性蛋白质和神经营养剂的救援作用。

• DOI: 10.1523/jneurosci.14-12-07629.1994
• 发表时间: 1994
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Yin,QW;Johnson,J;Prevette,D;Oppenheim,RW
• 通讯作者: Oppenheim,RW

The expression of trkB and p75 and the role of BDNF in the developing neuromuscular system of the chick embryo.

trkB 和 p75 的表达以及 BDNF 在鸡胚神经肌肉系统发育中的作用。

• DOI: 10.1242/dev.122.2.715
• 发表时间: 1996
• 期刊: Development (Cambridge, England)
• 作者: McKay,SE;Garner,A;Caldero,J;Tucker,RP;Large,T;Oppenheim,RW
• 通讯作者: Oppenheim,RW

Mechanisms of insulin-like growth factor regulation of programmed cell death of developing avian motoneurons.

类胰岛素生长因子调节发育中鸟类运动神经元程序性细胞死亡的机制。

• 发表时间: 1998
• 期刊: Journal of neurobiology.
• 作者: D'Costa,AP;Prevette,DM;Houenou,LJ;Wang,S;Zackenfels,K;Rohrer,H;Zapf,J;Caroni,P;Oppenheim,RW
• 通讯作者: Oppenheim,RW