MECHANISMS OF LOCAL SYNAPTIC TRANSMISSION IN DORSAL HORN

背角局部突触传递机制

• 批准号: 2265674
• 负责人: STEPHEN P SCHNEIDER
• 金额: $ 8.09万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2265674
• 关键词: antidromic impulse; axon; dorsal horn; electrophysiology; hamsters; immunocytochemistry; interneurons; neural conduction; neural information processing; neural plasticity; neural transmission; neuroanatomy; neuronal transport; neuropharmacology; reflex; sensorimotor system; spinal nerves; synapses

The long-term goal of this project is to understand the neural mechanisms of local synaptic circuits in the spinal dorsal horn that play vital roles in mediating segmental reflexes and integrating diverse types of incoming sensory information. In the next 3 years we propose to test the general hypothesis that neural networks are assembled from groups of interneurons expressing signature membrane properties and connectivity patterns that correlate with their mechanosensory input. Determining the functional and structural basis of these networks is essential for understanding central mechanisms that underlie long-term alteration of sensory and reflex function and contribute to neuropathic pain syndromes. The specific aims are to: l) Determine if interneurons that process specific mechanosensory modalities have intrinsic firing properties and ionic conductances different from interneurons that process other modalities. 2) Determine whether differential expression of ionic conductances in interneurons is related to differences in firing properties and/or axonal organization. 3) Determine the characteristics of synaptic transmission between individual pre- and postsynaptic neurons and identify the synaptic mediators involved. 4) Investigate plastic changes in interneuron firing properties and synaptic linkage. An integrated series of in vitro experiments are planned utilizing a unique rodent spinal cord preparation that allows functional identification of sensory inputs. Local circuit interneurons are studied using whole-cell recording methodology and are identified according to their axonal connections and functional inputs. Pharmacological techniques and immunohistochemical staining are used to provide data about the chemical neuroanatomy of network neurons. The results will contribute to a better understanding of network function in spinal cord neuropathies and will provide a blueprint for future efforts to model network information processing in the dorsal horn.

该项目的长期目标是了解神经机制 在脊柱背角中扮演至关重要的角色的局部突触电路 在中介节段反射和整合多种类型的传入中 感官信息。在接下来的三年中，我们建议测试一般 假设神经网络是由中间神经元组组装的 表达签名膜特性和连通性模式 与它们的机械感觉输入相关。确定功能和 这些网络的结构基础对于理解中心是至关重要的 长期改变感觉和反射的机制 功能并有助于神经性疼痛综合征。具体目标 是： l）确定该处理特定机械感应的中间神经元是否 模式具有内在的触发特性和离子电导 与处理其他方式的中间神经元不同。 2）确定离子电导的差异表达是否在 中间神经元与点火特性和/或轴突的差异有关 组织。 3）确定突触传播的特征 单个前后突触神经元并识别突触 涉及的调解人。 4）研究中间神经元点火特性的塑性变化和 突触连接。 计划利用一系列集成的体外实验 独特的啮齿动物脊髓制备 识别感觉输入。研究了本地电路中间神经元 使用全细胞记录方法，并根据 它们的轴突连接和功能输入。药理技术 免疫组织化学染色用于提供有关 网络神经元的化学神经解剖学。结果将有助于 更好地了解脊髓神经病的网络功能和 将为未来的努力提供建模网络信息的蓝图 在背喇叭中进行处理。