REGULATION OF NEURONAL PROLIFERATION AND DIFFERENTIATION

神经元增殖和分化的调节

• 批准号: 2266859
• 负责人: THOMAS A REH
• 金额: $ 18.96万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2266859
• 关键词: cell differentiation; cell growth regulation; cell type; central nervous system; embryo /fetus; epidermal growth factor; gene expression; germ layers; glia; growth factor receptors; in situ hybridization; laboratory rat; mitogens; neurogenesis; neurons; northern blottings; polymerase chain reaction; retina; tissue /cell culture; transforming growth factors

The central nervous system (CNS) has many neuronal cell types, and within a given brain region, these different types of neurons are present in precise ratios. Such regular ratios of neurons are characteristic of all brain regions and are fundamental to the proper functioning of the nervous system. However, virtually nothing is known of the mechanisms that regulate the production of the various types of neurons during the development of the CNS. Several lines of evidence indicate that this process is in some way controlled by the microenvironment of the developing CNS. The experiments outlined in this proposal will test several specific predictions of a model of central neurogenesis in which age-dependent changes in the retinal microenvironment determine the types of neurons that are produced at any given time. The experiments can be divided into two parts: 1. What factors regulate the proliferation of the germinal neuroepithelial cells during retinal histogenesis? 2. What are the cellular and molecular mechanisms involved in the generation of particular types of neurons/glia from the multipotent progenitor? An understanding of the factors that regulate CNS neuronal proliferation will undoubtedly prove invaluable in future attempts at reconstruction and regeneration of the damaged CNS as well as providing insight into the mechanisms of teratological agents that disrupt the normal development of the nervous system and neoplastic transformation of germinal neuroepithelial cells.

中枢神经系统（CNS）具有许多神经元细胞类型，并且 在给定的大脑区域内，这些不同类型的神经元是 以精确的比率存在。 这种定期的神经元比率是 所有大脑区域的特征，是 神经系统的正确功能。 但是，实际上 对调节生产的机制一无所知 中枢神经系统开发过程中各种类型的神经元。 几条证据表明，此过程以某种方式是 由发育中心的微环境控制。 这 该提案中概述的实验将测试一些特定的 预测中心神经发生模型，其中 视网膜微环境的年龄依赖性变化决定了 在任何给定时间产生的神经元类型。 这 实验可以分为两个部分：1。调节哪些因素 在 视网膜组织发生？ 2。什么是细胞和分子 特定类型的机制 来自多能祖细胞的神经元/神经元？对 调节CNS神经元增殖的因素将 毫无疑问，未来重建的尝试是无价的 以及受损的中枢神经系统的再生以及提供见解 进入破坏正常剂的脑病学剂的机制 神经系统的发展和肿瘤转化 生发神经上皮细胞。