METABOLISM AND NEUROCHEMISTRY OF LYSINE IN THE BRAIN

大脑中赖氨酸的代谢和神经化学

• 批准号: 2262353
• 负责人: YUNG-FENG CHANG
• 金额: $ 16.9万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-12-01 至 1996-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2262353
• 关键词: aminoacid metabolism; aminoacid oxidase; aminoacid transport; astrocytes; brain metabolism; cerebral cortex; chemical reaction; developmental neurobiology; enzyme activity; enzyme mechanism; gamma aminobutyrate; glutamates; immunocytochemistry; kynurenine; laboratory mouse; laboratory rabbit; laboratory rat; lysine; mitochondria; neurochemistry; neuropharmacology; peroxisome; piperidine; tissue /cell culture; transaminases

This research project continues to elucidate the reaction mechanisms involved in the metabolism of L-lysine to delta1-piperideine-2-carboxylic acid (P2C), P2C to L-pipecolic acid, L-pipecolic acid to delta1- piperideine-6-carboxylic acid (P6C), P6C to L-alpha-amonoadipic acid (AAA), and AAA to alpha-ketoadipic acid. It will isolate, purify to homogeneity and characterize L-lysine oxidase, P2C reductase, pipecolate oxidase, L-alpha-aminoadipic delta-semialdehyde (or P6C) oxidoreductase, and alpha-aminoadipate:alpha-ketoglutarate aminotransferase in the brain. It will isolate, purify and compare the characteristics of pipecolate oxidase localized in the peroxisomes and mitochondria. Mono- and polyclonal antibodies against L-lysine oxidase, P2C reductase, pipecolate oxidase, P6C oxidoreductase and alpha-aminoadipate:alpha-kekoglutarate aminotransferase in the rat brain will be prepared. The regional distributions of L-lysine oxidase, P2C reductase, pipecolate oxidase, P6C oxidoreductase and alpha-aminoadipate:alpha-kekoglutarate aminotransferase in the rat brain through enzyme activity study and immunocytochemical study. It will examine the developmental aspects of enzymes in the L-lysine pathway through enzyme activity assays and immunocytochemical study. It will examine the effect of L-alpha- aminoadipic acid and other lysine metabolites on the purified kynurenine aminotransferase and in astrocyte cultures. It will study the uptake of L-AAA in the astrocytes through cell cultures.

该研究项目继续阐明反应机制 参与L-赖氨酸的代谢到Delta1-Piperideine-2-羧基 酸（P2C），P2C至L-Pipecolic Acid，L-Pipecolic Acid到Delta1- Piperideine-6-羧酸（P6C），P6C至L-α-氨基脱磷酸 （AAA）和AAA到α-酮丹哌酸。 它将隔离，净化 同质性和表征L-赖氨酸氧化酶，P2C还原酶，圆柱状 氧化酶，L-α-氨基丙二醇二甲醛（或P6C）氧化还原酶， 和α-氨基磷酸酯：大脑中的α-酮戊二酸氨基转移酶。 它将隔离，纯化和比较圆柱状的特征 氧化酶位于过氧化物酶体和线粒体中。 单声和 抗L-赖氨酸氧化酶，P2C还原酶，圆柱状的多克隆抗体 氧化酶，P6C氧化还原酶和α-氨基磷酸：α-Kekoglutarate 将准备大鼠脑中的氨基转移酶。 区域 L-赖氨酸氧化酶，P2C还原酶，哌二醇氧化酶，P6C的分布 氧化还原酶和α-氨基磷酸：α-Kekoglutarate 通过酶活性研究和 免疫细胞化学研究。 它将检查 通过酶活性测定法和L-赖氨酸途径中的酶 免疫细胞化学研究。 它将检查l-alpha-的影响 纯化的kynurenine上的氨基丙酸和其他赖氨酸代谢产物 氨基转移酶和星形胶质细胞培养物。 它将研究 通过细胞培养物中星形胶质细胞中的L-AAA。