CEREBELLUM AND NM CONDITIONING IN ANESTHETIZED RABBITS

麻醉兔的小脑和 NM 调节

• 批准号: 2250435
• 负责人: Joseph Steinmetz
• 金额: $ 11.61万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2250435
• 关键词: anesthesia; arousal; brain electrical activity; central neural pathway /tract; cerebellum; conditioning; electrostimulus; eyelids; laboratory rabbit; learning; neural plasticity; nictitating membrane; nontherapeutic iontophoresis; single cell analysis

The long-term goal of research proposed in the present application is to delineate neuronal systems and processes involved in simple forms of learning and memory. More specifically, this research seeks to advance our understanding of the involvement of the cerebellum in a simple motor learning task, classical eyelid conditioning in rabbits. Three experiments are proposed that use anesthetized classical conditioning preparations. These preparations involve the pairing of brain microstimulation in place of peripheral conditioning stimuli and recording unit activity from brain stem nuclei known to be involved in generating conditioning responses in place of monitoring overt behavioral responses. The proposed experiments include: (1) An analysis of extracellular single unit cerebellar activity during and after conditioning, (2) Manipulations of the "behavioral" training regimen used during the anesthetized conditioning, and (3) Intracellular recordings coupled with HRP labeling after conditioning. These experiments have been designed to explore similarities and differences in conditioning between awake and anesthetized rabbits, to provide information about changes in the activity of cerebellar neurons induced by the anesthetized conditioning procedures, and to provide some insight into the connectivity between cerebellar and brain stem neurons involved in the conditioning. The further development of this anesthetized conditioning preparation should facilitate the definition and study of cerebellar and brain stem circuits activated during this form of learning. Intracellular analyses of neuronal activity should provide initial information about cellular processes and synaptic mechanisms associated with classical eyelid conditioning. We anticipate that these mechanisms may be generalizable to other motor learning and memory situations and therefore advance our understanding of basic cerebellar and brainstem neuronal function. Results from these experiments should also prove useful for understanding loss of normal function associated with cerebellar pathologies caused by injury or disease. Moreover, advancing our knowledge of cellular and synaptic processes involved in simple motor learning and memory should prove useful for understanding more complex learning and memory processes and the cellular bases of learning impairments and memory loss associated with disease and injury.

本应用中提出的研究的长期目标是 描述以简单形式的神经元系统和过程 学习和记忆。更具体地说，这项研究旨在推动我们的 了解小脑参与简单电机 学习任务，兔子的经典眼睑调节。三个实验 提出了使用麻醉的经典调节准备。 这些制剂涉及对脑微刺激的配对 大脑的外围调节刺激和记录单位活动 已知的茎核参与生成条件反应 监视明显的行为反应的地方。提出的实验 包括：（1）细胞外单个单位小脑活性的分析 调节期间和之后，（2）对“行为”的操纵 麻醉条件期间使用的训练方案，（3） 调节后的细胞内记录以及HRP标记。 这些实验旨在探索相似性和 清醒和麻醉兔子之间的调节差异， 提供有关小脑神经元活动变化的信息 由麻醉条件程序诱导，并提供一些 深入了解小脑和脑干神经元之间的连通性 参与条件。这种麻醉的进一步发展 调理准备应有助于定义和研究 在这种学习形式的过程中，小脑和脑干电路被激活。 神经元活性的细胞内分析应提供初始 有关相关的细胞过程和突触机制的信息 带有经典的眼睑调节。我们预计这些机制 可能可以推广到其他运动学习和记忆情况，并且 因此，促进了我们对基本小脑和脑干的理解 神经元功能。这些实验的结果也应证明有用 了解与小脑相关的正常功能的丧失 受伤或疾病引起的病理。而且，促进我们的知识 简单运动学习与细胞和突触过程的 记忆应该对理解更复杂的学习和 记忆过程以及学习障碍和记忆的细胞基础 与疾病和伤害有关的损失。