CEREBELLUM AND NM CONDITIONING IN ANESTHETIZED RABBITS

麻醉兔的小脑和 NM 调节

• 批准号: 2250435
• 负责人: Joseph Steinmetz
• 金额: $ 11.61万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2250435
• 关键词: anesthesia; arousal; brain electrical activity; central neural pathway /tract; cerebellum; conditioning; electrostimulus; eyelids; laboratory rabbit; learning; neural plasticity; nictitating membrane; nontherapeutic iontophoresis; single cell analysis

The long-term goal of research proposed in the present application is to delineate neuronal systems and processes involved in simple forms of learning and memory. More specifically, this research seeks to advance our understanding of the involvement of the cerebellum in a simple motor learning task, classical eyelid conditioning in rabbits. Three experiments are proposed that use anesthetized classical conditioning preparations. These preparations involve the pairing of brain microstimulation in place of peripheral conditioning stimuli and recording unit activity from brain stem nuclei known to be involved in generating conditioning responses in place of monitoring overt behavioral responses. The proposed experiments include: (1) An analysis of extracellular single unit cerebellar activity during and after conditioning, (2) Manipulations of the "behavioral" training regimen used during the anesthetized conditioning, and (3) Intracellular recordings coupled with HRP labeling after conditioning. These experiments have been designed to explore similarities and differences in conditioning between awake and anesthetized rabbits, to provide information about changes in the activity of cerebellar neurons induced by the anesthetized conditioning procedures, and to provide some insight into the connectivity between cerebellar and brain stem neurons involved in the conditioning. The further development of this anesthetized conditioning preparation should facilitate the definition and study of cerebellar and brain stem circuits activated during this form of learning. Intracellular analyses of neuronal activity should provide initial information about cellular processes and synaptic mechanisms associated with classical eyelid conditioning. We anticipate that these mechanisms may be generalizable to other motor learning and memory situations and therefore advance our understanding of basic cerebellar and brainstem neuronal function. Results from these experiments should also prove useful for understanding loss of normal function associated with cerebellar pathologies caused by injury or disease. Moreover, advancing our knowledge of cellular and synaptic processes involved in simple motor learning and memory should prove useful for understanding more complex learning and memory processes and the cellular bases of learning impairments and memory loss associated with disease and injury.

本申请提出的研究的长期目标是 描绘涉及简单形式的神经元系统和过程 学习和记忆。更具体地说，这项研究旨在推进我们的 了解小脑在简单运动中的参与 学习任务，兔子的经典眼睑调节。三个实验 建议使用麻醉经典调理制剂。 这些准备工作涉及大脑微刺激的配对 外周调节刺激和记录大脑的单位活动 已知干细胞核参与产生调节反应 监视明显行为反应的地方。拟议的实验 包括：（1）细胞外单单位小脑活动分析 在调节期间和之后，（2）“行为”的操纵 麻醉调理期间使用的训练方案，以及 (3) 调节后细胞内记录与 HRP 标记相结合。 这些实验旨在探索相似之处和 清醒和麻醉兔子之间的调节差异 提供有关小脑神经元活动变化的信息 由麻醉调理程序引起，并提供一些 深入了解小脑和脑干神经元之间的连接 参与调理。这种麻醉剂的进一步发展 调理准备应有助于定义和研究 在这种形式的学习过程中，小脑和脑干回路被激活。 神经元活动的细胞内分析应提供初步的 有关细胞过程和相关突触机制的信息 与经典的眼睑调理。我们预计这些机制 可以推广到其他运动学习和记忆情况 因此增进我们对基本小脑和脑干的理解 神经元功能。这些实验的结果也应该被证明是有用的 用于了解与小脑相关的正常功能丧失 损伤或疾病引起的病理。此外，还可以提高我们的知识水平 参与简单运动学习的细胞和突触过程 记忆应该被证明对于理解更复杂的学习和 记忆过程以及学习障碍和记忆的细胞基础 与疾病和伤害相关的损失。