PCB, PCDD, PCDF, AND PAH EXPOSURE FROM HAZARDOUS WASTE AMONG MOHAWK INDIANS

莫霍克印第安人接触危险废物的 PCB、PCDD、PCDF 和 PAH

• 批准号: 3840813
• 负责人: EDWARD F FITZGERALD
• 金额: --
• 依托单位: STATE UNIVERSITY OF NEW YORK AT ALBANY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3840813
• 关键词: Native Americans; air sampling /monitoring; biomarker; blood chemistry; breast feeding; breath tests; carbopolycyclic compound; cytochrome P450; dioxins; embryo /fetus toxicology; environmental contamination; environmental toxicology; enzyme induction /repression; epidemiology; feces analysis; halobiphenyl /halotriphenyl compound; human milk; human pregnant subject; human subject; industrial waste; infant human (0-1 year); mother /embryo /fetus nutrition; nutrition related tag; polychlorodibenzofuran; pregnancy circulation; soil pollution; soil sampling; statistics /biometry; toxin metabolism; umbilical cord; urinalysis; waste disposal; water pollution; water sampling /testing; water supply

An epidemiologic study is proposed of Mohawk women and infants to test the hypotheses that exposure to polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), and polynuclear aromatic hydrocarbons (PAHs) from a nearby Superfund hazardous waste site elevates body burdens and affects the cytochrome P-450-dependent mixed function monooxygenase system. Exposure and body burdens will be assessed by: 1) estimating cumulative maternal dose from ingestion, inhalation, and dermal contact; 2) measuring concentrations of 68 routine PCB congeners in serum and urine, and, if lactating, of an additional 12 coplanar PCB congeners and of 34 PCDD/F congeners and 15 PAHs in breast milk; and 3) measuring infant concentrations of the PCB congeners in cord serum and urine and output of the PCDD/F congeners in feces. P-450IA2 activity in the mothers and infants will be assessed through a caffeine breath test, a safe and noninvasive procedure that measures the rate at which labelled carbon dioxide is exhaled as an indirect monitor. Pregnant Mohawk women will be interviewed and invited to participate in maternal blood sampling during their second trimester and cord blood sampling at delivery. They will also be asked to provide blood, urine, and breast milk samples and to collect infant urine and feces in cloth diapers at 1, 3, and 6 months post-partum to investigate change in pollutant levels over time. Multivariable statistical techniques will be used: 1) to test for differences in the mean contaminant levels of mothers according to cumulative dose and of infants according to breast-feeding status; 2) to relate breast milk pollutant concentrations to maternal and infant urinary levels and infant fecal output; and 3) to correlate P-450IA2 activity with levels of PCB and PCDD/F congeners and PAHs. A novel statistical technique based on summary scores will be used to "fingerprint" the waste site as a point source. The results will significantly enhance our understanding of how important classes of chemicals arising from hazardous waste bioaccumulate in human adults and infants and whether P-450IA2 induction is a sensitive biomarker of their early biologic effect.

提出了一项关于莫霍克族妇女和婴儿的流行病学研究，以测试 假设暴露于多氯联苯（PCB）， 多氯联苯二二氧蛋白和Dibenzofurans（PCDD/FS），以及 来自附近超级基金有害的多核芳族碳氢化合物（PAHS） 废物站点增加了身体负担，并影响细胞色素P-450依赖性 混合功能单加氧化酶系统。 暴露和身体负担将是 评估者：1）从摄入中估算累积母体剂量， 吸入和真皮接触； 2）测量68个例程的浓度 精华和尿液中的PCB同类物，如果哺乳，则有12个 共面PCB同源物和34位PCDD/F同源物和15个PAHS乳房 牛奶; 3）测量绳索中PCB同源物的婴儿浓度 粪便中PCDD/F同源物的血清和尿液和输出。 P-450IA2 母亲和婴儿的活动将通过咖啡因进行评估 呼气测试，一种安全且无创的程序，可用于测量速率 将标记为二氧化碳的标记为间接监视器。 孕 莫霍克族妇女将接受采访并邀请参加母亲 在第二个孕期进行血液采样和脐带血采样 送货。 他们还将被要求提供血液，尿液和母乳 样品并在1、3的布尿布中收集婴儿尿液和粪便，然后 产后6个月以调查污染物水平随时间变化的变化。 将使用多变量统计技术：1）测试 母亲平均污染物水平的差异 根据母乳喂养状态的累积剂量和婴儿； 2）到 将母乳污染物浓度与母亲和婴儿尿 水平和婴儿粪便输出； 3）将P-450IA2活性与 PCB和PCDD/F同源物和PAH的水平。 一种新颖的统计技术 根据摘要，分数将用于将废物现场“指纹”作为一个 点源。 结果将大大增强我们对 危险废物产生的化学物质的重要类别 人类和婴儿的生物积累以及P-450IA2诱导是否是 其早期生物学作用的敏感生物标志物。