CENTER FOR FAST KINETICS RESEARCH

快速动力学研究中心

• 批准号: 3103705
• 负责人: ANTHONY HARRIMAN
• 金额: $ 54.59万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1975
• 资助国家: 美国
• 起止时间: 1975-06-01 至 1994-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3103705
• 关键词: biomedical equipment resource; confocal scanning microscopy; flash photolysis; fluorescence spectrometry; nuclear medicine; online computer; pulse radiolysis

During the next funding period the Center for Fast Kinetics Research (CFKR) will continue to develop its sophisticated array of computerized instruments for the generation and measurement of transient species. These experimental capabilities, together with the expertise of the CFKR staff, will be made available to the scientific community. The CFKR will advertise its capabilities in an effort to open the methods of fast kinetics to as wide an audience as possible. To this end, the CFKR has appointed a new advisory board, demonstrating far greater responsibility and involvement than apparent in earlier years. In order to address specific scientific problems, e.g., rapid energy and electron transfers in biological and biomimetic systems, time-resolved fluorescence from living cells and other organisms, characterization of transient species in opaque materials, observation of weakly-absorbing intermediates, selective excitation of chromophores in proteins, measurement of the energy content of transient species, etc., the CFKR seeks to upgrade and extend its instrumentation. In particular, new detection methods will be implemented consisting of diffuse reflectance spectroscopy, photoconductivity, thermal lensing, and photo-acoustic calorimetry. Substantial modifications will be made in the area of time-resolved fluorescence spectroscopy, in order to improve its existing reliability and resolution (both temporal and spectral). Construction of a novel confocal microscope will be undertaken; this instrument will permit video scanning of the specimen via a linear servo galvanometer system, photoablation through an acousto-optic modular, and time-resolved fluorescence spectroscopy throughout the entire UV-VIS spectrum with time and wavelength resolutions of 5 ps and 5 nm respectively. The available facilities for preparation and handling of biological samples will be extended. Also, control of all experiments will be transferred from the existing PDP 11/70 minicomputer to a dedicated array of microcomputers, thus minimizing down-time, increasing software flexibility, and removing the need for costly maintenance agreements. Further aims for the next funding period include the undertaking of interdisciplinary collaborations with outside researchers in fields relating to biomedical problems, maintaining a close watch for searchers in fields relating to biomedical problems, maintaining a close watch for technological advances of relevance in the field of fast kinetics, and ensuring that the biomedical fraternity are kept fully informed our resources and capabilities.

在下一个资金期间，快速动力学研究中心 （CFKR）将继续开发其复杂的计算机化阵列 瞬态物种生成和测量的仪器。 这些实验能力以及CFKR的专业知识 工作人员将提供给科学界。 CFKR将 宣传其功能以打开快速的方法 动力学尽可能广泛。为此，CFKR有 任命了一个新的顾问委员会，表明了更大的责任 和参与比早期的明显。为了解决 特定的科学问题，例如快速能量和电子传输 在生物学和仿生系统中，从 活细胞和其他生物，瞬态物种的表征 在不透明的材料中，观察弱吸收中间体的观察 蛋白质中发色团的选择性激发，测量 瞬时物种等的能源含量，CFKR试图升级和 扩展其仪器。特别是，新的检测方法将是 由弥散反射光谱组成的实施， 光电导率，热透镜和光声量热法。 将在时间分辨的领域进行实质性修改 荧光光谱，以提高其现有可靠性 和分辨率（时间和光谱）。构造小说 将进行共聚焦显微镜；该乐器将允许视频 通过线性伺服电量计系统扫描样品， 通过声学模块化和时间分辨的光绘 随着时间的流逝，整个UV-VIS光谱中的荧光光谱 分别分别为5 ps和5 nm的波长分辨率。可用 准备和处理生物样品的设施将是 扩展。同样，所有实验的控制将从 现有的PDP 11/70微型计算机到专用的微型计算机， 从而最大程度地减少停机时间，提高软件灵活性并删除 需要昂贵的维护协议。进一步的目标 资金期包括跨学科的承诺 与与生物医学有关的领域的外部研究人员的合作 问题，保持与有关字段中有关的搜索者的密切监视 生物医学问题，保持密切关注技术进步 在快速动力学领域的相关性，并确保 生物医学兄弟会被充分了解我们的资源和 功能。