NATIONAL TRITIUM LABELING FACILITY

国家氚标记设施

• 批准号: 3103813
• 负责人: DAVID E WEMMER
• 金额: $ 59.27万
• 依托单位: UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3103813
• 关键词: biomedical resource; chemical synthesis; radiopharmacology; radiotracer; tritium

The National Tritium Labeling Facility (NTLF) was funded on 08/01/82. During its first year the facility was built and equipped; it became fully operative in August 1983. It is now engaged in an active program of aiding biomedical researchers to obtain needed and commercially-unavailable tritium labeled compounds. For the ten month period Oct. 1983-Aug. 1984 this has averaged two labeling operations per month and has aided scientists from as far away as New York and Hawaii. The compounds labeled have included prednisone, nicotine, pheromones, gibberelic acids, endorphins, tabtoxinine- -lactam, and tuftsin. The tritium facility is now fully staffed, and because knowledge of its labeling services is growing, requests for tritiation help are also growing; the facility is expected to reach its full capacity (one tritiation per week) in its 3rd year. The core research is being rapidly pursued. During the 2nd grant year one manuscript was accepted for publication in the Journal of Physical Chemistry, and a second manuscript was submitted to that journal in September 1984. The core research summarized in these manuscripts contributes to our understanding of the processes involved in "excitation labeling" (exposure of organic compounds to a stream of T2 that has been passed through a microwave generator). The knowledge gained in this work has increased the efficiency with which the excitation procedure can be used. This research will be enhanced in the 3rd and succeeding grant years by the tritium-NMR capability that has been added. The position(s) of tritium in all labeled compounds can now be determined, and this greatly aids the mechanistic interpretations regarding the tritiation procedures that have been used. The staff of the NTLF is doing collaborative research (co-authorship of published papers) with Professor Richard E. Moore of the University of Hawaii; the work is directed towards identifying the cell-membrane receptor sites for the potent tumor promoters lyngbyatoxin and aplysiatoxin. The staff is also collaborating with Professor A.J. Kresge of the University of Toronto on hydrogen-isotope effects on NMR spectra. This kind of collaborative research will be expanded with the increasing use of the NTLF by biomedical researchers.

国家tri列标签设施（NTLF）于08/01/82资助。 在第一年，该设施是建造和配备的；它变得完全 1983年8月的手术人员。它现在正在进行一个积极的协助计划 生物医学研究人员获得所需和商业上可用的 Tritium标记化合物。 在1983年10月的十个月期间。 1984 这平均每月进行了两个标签操作，并有助于 远至纽约和夏威夷的科学家。 标记的化合物 已经包括泼尼松，尼古丁，信息素，gibberelicac Acids， 内啡肽，Tabtoxinine -lactam和tuftsin。 Tritium设施现在 配备员工，并且由于其标签服务的知识正在增长，所以 提出帮助的要求也在增长；该设施有望 在第三年达到其全部容量（每周一次）。 核心研究正在迅速进行。 在第二年的第二年 手稿在《物理杂志》杂志上被接受 化学和第二个手稿在该期刊上提交 1984年9月。核心研究总结在这些手稿中 有助于我们理解“激发”所涉及的过程 标签“（有机化合物暴露于已有的T2流 通过微波生成器）。 这项工作中获得的知识 提高了激发程序的效率 用过的。 这项研究将在第三和随后的赠款年中得到增强 通过添加的Tritium-NMR功能。 位置 现在可以确定所有标记化合物中的tri剂 有助于有关tritiation程序的机械解释 已经使用了。 NTLF的工作人员正在进行合作研究（合着者 发表论文）与大学的理查德·E·摩尔教授 夏威夷；该工作针对识别细胞膜受体 有效的肿瘤启动子林尼毒素和丙酰胺毒素的位点。 这 员工还与A.J.教授合作大学 多伦多对NMR光谱的影响。 这种 随着NTLF的越来越多使用，协作研究将扩大 由生物医学研究人员。