LUMICKS C-trap for Mechanistic Studies of Biological Systems at the University of Rochester

罗切斯特大学用于生物系统机械研究的 LUMICKS C-trap

• 批准号: 10435816
• 负责人: Elio Aaron Abbondanzieri
• 金额: $ 60万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10435816
• 关键词: Address; Bacteria; Biological Assay; Biological Process; CRISPR/Cas technology; Cells; Chromatin Structure; Custom; Funding; Future; Genetic Transcription; Goals; Institutes; Measurement; Measures; Mechanics; Medical center; Microfluidics; New York; Organelles; Phase; Regulation; Research; Research Personnel; Resources; Rivers; Services; System; Techniques; Technology; Translations; United States National Institutes of Health; Universities; base; biological systems; cell motility; design; fluorescence microscope; fluorophore; instrument; interest; laser tweezer; mechanical force; optical traps; protein folding; single molecule; tool development; user friendly software

Abstract We are requesting funding to purchase a LUMICKS C-Trap optical tweezer and confocal fluorescence microscope. This instrument will greatly expand the ability to perform mechanical measurements of single molecules and cells at the University of Rochester. These services will be made available to a user base extending throughout the University of Rochester and Western New York. Mechanical measurements of single molecules and cells greatly advance our understanding of fundamental biological processes such as cell motility, transcription, and protein folding. However, optical tweezers are frequently custom-designed, making it difficult for non- specialists to adapt these techniques to their system of interest. The LUMICKS C-Trap will remove this barrier by providing a versatile microfluidic delivery system and user-friendly software that will serve as a single integrated platform for applying a wide range of mechanical forces, precisely detecting nm-scale structural transitions, and measuring the emission of multiple fluorophores concurrently. Dr. Elio Abbondanzieri, a pioneer in the field of optical tweezers, will assist users in designing assays tailored to their scientific questions. The broad range of measurements that can be performed with this instrument will be utilized by our diverse initial user group covering a wide range of scientific topics including phase-separated condensates from bacteria, regulation of translation, CRISPR tool development, cell migration, chromatin structure, and organelle motility. This group includes researchers from the University of Rochester River Campus, University of Rochester Medical Center, and Rochester Institute of Technology. Although the initial users have varied interests, a common theme is that they have scientific questions that cannot be addressed with instruments currently available on campus. The LUMICKS C-Trap is thus ideally suited to function as a state-of-the-art resource that enables current and future investigators to further the goals of their NIH-funded research.

抽象的 我们正在申请资金购买 LUMICKS C-Trap 光镊和共焦 荧光显微镜。该仪器将极大地扩展执行机械操作的能力 罗切斯特大学的单分子和细胞测量。这些服务将 可供整个罗切斯特大学和西安大略大学的用户群使用 纽约。单分子和细胞的机械测量极大地推进了我们的研究 了解基本的生物过程，例如细胞运动、转录和蛋白质 折叠式的。然而，光镊通常是定制设计的，这使得非专业人士很难 专家使这些技术适应他们感兴趣的系统。 LUMICKS C-Trap 将去除 通过提供多功能微流体输送系统和用户友好的软件来克服这一障碍 作为一个单一的集成平台，可以精确地施加各种机械力 检测纳米级结构转变，并测量多个荧光团的发射 同时。光镊领域的先驱Elio Abbondanzieri博士将协助用户 设计适合他们的科学问题的分析方法。广泛的测量范围 使用该仪器进行的操作将被我们多元化的初始用户群所使用，涵盖广泛 一系列科学主题，包括细菌的相分离冷凝物、调节 翻译、CRISPR 工具开发、细胞迁移、染色质结构和细胞器运动。 该小组包括来自罗彻斯特大学河校区、 罗彻斯特医学中心和罗彻斯特理工学院。尽管最初的用户已经 兴趣各异，但共同的主题是他们有无法解决的科学问题 使用校园内现有的仪器。因此，LUMICKS C-Trap 非常适合 作为最先进的资源，使当前和未来的研究人员能够进一步推进 NIH 资助的研究目标。