IMMUNOREGULATORY DEFECTS IN HEMOPHILIA

血友病的免疫调节缺陷

• 批准号: 2220363
• 负责人: JOHN Lewis SULLIVAN
• 金额: $ 26.11万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2220363
• 关键词: Epstein Barr virus; HIV infections; antibody neutralization test; cytotoxic T lymphocyte; disease /disorder proneness /risk; helper T lymphocyte; hemophilia As; host organism interaction; human immunodeficiency virus 1; human subject; immunoregulation; longitudinal human study; lymphocyte proliferation; microorganism immunology; mixed lymphocyte reaction test; neutralizing antibody; polymerase chain reaction; tissue /cell culture; virus protein; Epstein Barr virus; HIV infections; antibody neutralization test; cytotoxic T lymphocyte; disease /disorder proneness /risk; helper T lymphocyte; hemophilia As; host organism interaction; human immunodeficiency virus 1; human subject; immunoregulation; longitudinal human study; lymphocyte proliferation; microorganism immunology; mixed lymphocyte reaction test; neutralizing antibody; polymerase chain reaction; tissue /cell culture; virus protein

The long term objectives of this project are to understand the immunological and virological factors involved in the pathogenesis of HIV-1 infection. The information will be useful in the development of an effective vaccine for prevention of HIV infection. The major hypothesis to be tested is that strong virus specific CTL responses and neutralizing antibodies are associated with a delayed progression of HIV- 1 induced immune attrition. These studies will focus on a cohort of 164 patients with hemophilia, 73 percent of whom have been infected with HIV- 1 since 1981-83. In this cohort we have identified HIV-1 infected non- progressors, slow progressors and rapid progressors. Immunological and virological studies will be carried out on fresh and repository samples of sequential plasma and peripheral blood mononuclear collected prospectively during the course of the project. These investigators propose that HIV-specific cytotoxic T lymphocyte responses against a panel of HIV-1 proteins and neutralizing antibodies against autologous viral isolates. Viral isolates will be characterized for cytopathic Epstein Barr virus and measure CD4 T helper cell function to evaluate non-HIV directed defenses.

该项目的长期目标是了解 涉及的免疫学和病毒学因素参与 HIV-1感染。 该信息将在开发中有用 一种预防HIV感染的有效疫苗。 专业 要检验的假设是强大的病毒特异性CTL反应和 中和抗体与HIV的延迟进展有关 1诱导免疫损耗。 这些研究将集中于164的队列 血友病患者，其中73％感染了HIV- 1自1981 - 83年以来。 在此队列中，我们已经确定了HIV-1感染的非 - 进步者，慢速进步者和快速进步者。 免疫学和 病毒学研究将在新鲜和存储库样品上进行 序性血浆和外周血单核收集 在项目过程中前景。 这些调查人员 提出针对A的HIV特异性细胞毒性T淋巴细胞反应 HIV-1蛋白的面板和对自体的中和抗体 病毒分离株。 病毒分离株将用于细胞质 Epstein Barr病毒并测量CD4 T辅助细胞功能以评估 非HIV的指示防御措施。