University of Washington Arboviral Research Network (UWARN)

华盛顿大学虫媒病毒研究网络 (UWARN)

基本信息

批准号：
10393401
负责人：
Michael Gale
金额：
$ 17.65万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-05-22 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10393401
关键词：
Acute Address Administrative Coordination Alphavirus Animals Antibodies Antiviral Agents Arbovirus Infections Arboviruses Area Arthropod Vectors Back Bacteriology Binding Biological Assay Biological Markers Blood Body Fluids Brazil Cell Separation Chikungunya virus Clinical Clinical Management Clinical Trials Collaborations Collection Congo Containment Data Dengue Dengue Infection Dengue Virus Detection Development Diagnosis Diagnostic Diagnostic Reagent Disease Emergency Situation Emerging Communicable Diseases Engineering Enzyme-Linked Immunosorbent Assay Epitopes Far East Fever Flavivirus Fluorescence Freezing Future Genomic approach Human Immune response Immune system Immunoglobulin G Individual Infection Infectious Diseases Research Innate Immune Response Innate Immune System Institutes Institution International Intervention Investigation Japanese Encephalitis Knowledge Laboratories Laboratory Research Lead Light Logistics Measurement Metagenomics Microcephaly Molecular Monitor Monoclonal Antibodies Natural Immunity Oropouche virus Orthobunyavirus Outcome Pakistan Parasitic infection Pathogenesis Pathway interactions Patients Population Prevalence Process Production Protein Engineering Proteins Readiness Reagent Research Resources Rift Valley Fever Risk Sampling Senegal Sensitivity and Specificity Serology Seroprevalences Site South Africa South America Specimen Statistical Data Interpretation Structure System Taiwan Technology Technology Transfer Testing The science of Mycology Therapeutic Time Universities Vaccines Variant Veterinarians Viral Viral Antibodies Viral Hemorrhagic Fevers Viral Load result Virus Virus Diseases Washington Yellow Fever ZIKA Zika Virus arthropod-borne base biobank candidate marker chikungunya cohort cross reactivity data management design experience global health global health emergency human monoclonal antibodies improved improved outcome innovation neutralizing antibody neutralizing monoclonal antibodies next generation sequencing novel novel diagnostics novel strategies pandemic disease pandemic preparedness pathogen pathogenic virus point of care population based prospective public health relevance severe dengue therapeutic target transcriptomics viral detection virology virus host interaction

项目摘要

Abstract: The United World Antiviral Research Network (UWARN, formerly referred to as the University of Washington Arboviral Research Network) will address emerging and re-emerging infectious diseases by carrying out research on arboviruses that include current high burden pathogens including dengue, chikungunya, and Zika viruses. Arboviruses also include emerging and re-emerging viruses such as Mayaro, Una, Usutu, Japanese encephalitis, yellow fever, Crimean-Congo hemorrhagic fever, and Oropouche viruses. UWARN will leverage the UW Alliance (formerly Metacenter) for Pandemic Disease Preparedness and strong research laboratory partners in Brazil, Senegal, South Africa, Pakistan and Taiwan as well as the UW Department of Global Health global reach in capacity building. The UWARN international Collaborating Partners have institution-based and population-based cohorts in place to carry out the planned research and monitor for new virus emergence. The proposed UWARN research will create new human viral-neutralizing monoclonal antibodies (Hu-nMabs) that can be used as therapeutics or diagnostics. UWARN research will also create new diagnostics, created ab initio by the Institute for Protein Design, as artificial proteins that release light when antibodies to virus are present in body fluids. Finally, UWARN research will generate an understanding of how viruses manipulate the human innate immune system, and this information will be used to design biomarkers to predict severe disease as well as to suggest host-directed therapies that could lead to better outcomes after arboviral infection. The proposed technologies are generic and could be rapidly adapted to any future emerging arbovirus threat. UWARN collaborators span virology, bacteriology, mycology, and parasitic infections, such that UWARN can respond to diverse emerging infectious diseases threats. UWARN international laboratories have advanced capabilities including biorepositories with generator-power backed-up -80C freezers, next-generation sequencing (NGS) capabilities, fluorescence cell sorting, and BSL-3 containment facilities. UWARN cohorts have biobanked samples taken from individuals during acute febrile illnesses, with known and unknown arboviral diseases, and partnership sites are poised for prospective collection of population and facility–based samples. UWARN labs will use metagenomic NGS to detect novel viral emergence in affiliated cohorts. UWARN Partners have collaborating entomologists and veterinarians to sample arthropod vectors and animal reservoirs as needed. Additional innovative capacity included the Brazil UWARN partner’s mobile van with on board NGS capability for real-time, on-site viral sequencing and sampling to viral identification in less than 18 hrs. A second partner, IRESSEF in Senegal, plans to set up a similar mobile vehicle for viral sampling and sequencing in Senegal and the Brazil group will collaborate with IRESSEF on technology transfer and best practices. UWARN partners will serve as a resource to other Emerging Infectious Diseases Research Centers (EIDRC) providing expertise in monoclonal antibodies, protein design, and host directed therapies.

摘要：联合世界抗病毒研究网络（UWARN，以前称为华盛顿大学虫媒病毒研究网络）将通过对虫媒病毒进行研究来应对新出现和重新出现的传染病，其中包括当前高负荷病原体，包括登革热、基孔肯雅热和寨卡病毒还包括新出现和重新出现的病毒，例如 Mayaro、Una、Usutu、日本脑炎、黄热病、克里米亚-刚果出血热病毒。 UWARN 将利用威斯康星大学流行病防备联盟（前身为 Metacenter）和巴西、塞内加尔、南非、巴基斯坦和台湾的强大研究实验室合作伙伴以及威斯康辛大学全球卫生部的全球影响力进行能力建设。 UWARN 国际合作伙伴拥有基于机构和人群的队列，以开展计划中的研究并监测新病毒的出现。拟议的 UWARN 研究将创造新的人类病毒中和剂。可用作治疗或诊断的单克隆抗体（Hu-nMab）也将创建新的诊断方法，由蛋白质设计研究所从头开始创建，作为当体液中存在病毒抗体时释放光的人造蛋白质。最后，UWARN 研究将深入了解如何操纵人类先天免疫系统中的病毒，这些信息将用于设计生物标志物来预测严重疾病，并建议针对宿主的治疗，从而在治疗后获得更好的结果。所提出的技术是通用的，可以快速适应任何未来出现的虫媒病毒威胁，UWARN 合作者涵盖病毒学、细菌学、真菌学和寄生虫感染，因此 UWARN 可以应对各种新出现的传染病威胁。功能包括带发电机备用 -80C 冰箱的生物样本库、下一代测序 (NGS) 功能、荧光细胞分选和 BSL-3 密封UWARN 队列拥有从患有已知和未知虫媒病毒疾病的急性发热性疾病个体中采集的样本，并且合作伙伴站点已准备好前瞻性地收集人群和基于设施的样本，UWARN 实验室将使用宏基因组 NGS 来检测新型病毒的出现。 UWARN 合作伙伴拥有合作的昆虫学家和兽医，可根据需要对节肢动物媒介和动物宿主进行采样。巴西 UWARN 合作伙伴的其他创新能力也包括在内。具有车载 NGS 功能的移动货车，可在 18 小时内进行实时现场病毒测序和采样，以进行病毒鉴定第二个合作伙伴塞内加尔的 IRESSEF 计划在塞内加尔建立类似的移动车辆，用于病毒采样和测序。塞内加尔和巴西集团将与 IRESSEF 合作进行技术转让和最佳实践，UWARN 合作伙伴将作为其他新兴传染病研究中心 (EIDRC) 的资源，提供单克隆抗体、蛋白质方面的专业知识。设计和主持定向疗法。