HIGH-RESOLUTION EXTENDED DNA MAPPING OF YACS

YACS 的高分辨率扩展 DNA 作图

• 批准号: 2209211
• 负责人: BRAD E. WINDLE
• 金额: $ 7.5万
• 依托单位: CTRC RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-12-01 至 1996-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2209211
• 关键词: DNA; artificial chromosomes; computer assisted sequence analysis; computer program /software; digital imaging; fluorescence microscopy; fluorescent dye /probe; genetic mapping; genetic techniques; genome; human genetic material tag; in situ hybridization; method development; molecular cloning; nucleic acid probes; nucleic acid structure; polymerase chain reaction; DNA; artificial chromosomes; computer assisted sequence analysis; computer program /software; digital imaging; fluorescence microscopy; fluorescent dye /probe; genetic mapping; genetic techniques; genome; human genetic material tag; in situ hybridization; method development; molecular cloning; nucleic acid probes; nucleic acid structure; polymerase chain reaction

The first goal of this project is to assess the feasibility of using a newly developed, rapid, high resolution, visual mapping procedure for the mapping of yeast artificial chromosomes. The assembly of maps from yeast artificial chromosomes for the human genome will facilitate identification of genetic disorders and the gene responsible. The second goal is to improve this new mapping procedure by developing a multi-color multi-probe strategy and developing computer software capable of analyzing the visual images that are generated. These improvements will allow faster mapping as well as provide the ability to rapidly and precisely detect gene rearrangements involved in human disorders. The new mapping technology that will be used involves the stretching of human genomic DNA on a slide and hybridizing fluorescent DNA probes to the extended DNA strands. The result is a multi-color string as viewed through a fluorescence microscope and represent a map of the DNA region of interest. Resolution as low as .4 Kb has been obtained. Yeast artificial chromosomes from human chromosome 3 will be studied with this technology.

该项目的第一个目标是评估使用 新开发，快速，高分辨率，视觉映射过程 酵母人工染色体的映射。 酵母中的地图组装 人工染色体的人类基因组将有助于 遗传疾病的鉴定和负责的基因。 第二个 目标是通过开发多色来改善这种新的映射程序 多探针策略和开发能够的计算机软件 分析生成的视觉图像。 这些改进将会 允许更快的映射以及提供快速和快速的能力 精确检测到与人类疾病有关的基因重排。 将使用的新映射技术涉及 载玻片上的人类基因组DNA并杂交荧光DNA探针至 扩展的DNA链。 结果是一个多色字符串 通过荧光显微镜并表示DNA区域的图 感兴趣的。 已经获得低至0.4 kb的分辨率。 酵母 将研究来自人类染色体3的人造染色体 技术。