CORE--STEM CELLS

核心--干细胞

• 批准号: 5205910
• 负责人: STEPHEN J FORMAN
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5205910
• 关键词: biomedical facility; cooperative study; hematopoietic stem cells

The role of this core in this grant proposal is to coordinate the collection, transduction and subsequent reinfusion of mobilized peripheral blood stem cells (PBSC) in individuals who are HIV-1 positive and who will be receiving a reinfusion of these cells after either retroviral or adeno- associated virus (AAV) transduction. The specific aims of the core are: 1) to coordinate the G-CSF mobilization of PBSC from HIV-1 positive infected individuals; 2) to collect and isolate CD34+ PBSC mobilized from such HIV-1 infected subjects; 3) to distribute CD34+ PBSC to Projects 2 and 3 for transduction of antiviral HIV-1 ribozymes utilizing either retroviral or AAV vectors; 4) to perform colony assays to confirm efficiency of transduction of these cells; 5) to provide PBSC and other clinical specimens to the Virology Core for HIV-1 marker analysis; 6) to coordinate laboratory analysis of peripheral blood samples from patients within Projects 2 and 3 who have received CD34+ transduced cells for analysis of DNA and of trans-gene expression. This core takes advantage of the extensive experience of the City of Hope Hematology and Bone Marrow Transplantation program in the techniques related to the procurement of PBSC utilized in transplantation for treatment of solid tumors and hematologic malignancies. Thus, these studies will be able to compare the degree of CD34+ cell enrichment observed in HIV-1 infected individuals compared to non-HIV-1 infected donors.

该核心在该赠款建议中的作用是协调 收集，转导和随后的动员外周的再灌注 HIV-1阳性的个体中的血液干细胞（PBSC） 在逆转录病毒或腺 相关病毒（AAV）转导。 核心的具体目的是： 1）协调HIV-1阳性PBSC的G-CSF动员 感染的个体； 2）收集和隔离CD34+ PBSC 此类HIV-1感染受试者； 3）将CD34+ PBSC分发给项目2 3用于使用任何一种的抗病毒HIV-1核酶转导 逆转录病毒或AAV载体； 4）进行殖民测定以确认 这些细胞转导的效率； 5）提供PBSC和其他 HIV-1标记分析的病毒学核心的临床标本； 6）到 协调患者外周血样本的实验室分析 在已收到CD34+转导的细胞的项目2和3中 DNA和跨基因表达的分析。 该核心利用了希望之城的丰富经验 技术中的血液学和骨髓移植程序 与用于移植的PBSC的采购有关 治疗实体瘤和血液学恶性肿瘤。 因此，这些 研究将能够比较CD34+细胞富集的程度 与未感染的非HIV-1相比，在HIV-1感染的个体中观察到 捐助者。