BIOCARS--A SYNCHROTRON STRUCTURAL BIOLOGY RESOURCE

BIOCARS--同步加速器结构生物学资源

• 批准号: 2283561
• 负责人: JOHN Keith MOFFAT
• 金额: $ 230.68万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-15 至 1997-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2283561
• 关键词: X ray; X ray crystallography; biomedical equipment resource; cooperative study; particle accelerators; structural biology

We wish to design, construct and operate a single sector at the Advanced Photon Source (APS) under construction at Argonne National Laboratory. The sector will consist of an undulator and wiggler source, a bending magnet source, associated x-ray optical elements, beam transport, three hutches, a BL3 biohazards facility, and experimental apparatus suitable for five different styles of experiments in structural biology: virus crystallography, small angle scattering, protein crystallography/multiple wavelength anomalous dispersion, time-resolved diffraction, and diffraction from microcrystals. The sector will be operated for the 29-member group of structural biologists denoted BioCARS (roughly 75% of the available beamtime) and for general users (roughly 25%). Technological research and development will concentrate on: X-ray optics, including transmission optics and cooling schemes that will enable the optics to withstand the high heat loads imposed by the insertion device sources without significant degradation of their properties; storage phosphor and other detectors; sample handling devices such as liquid helium cryostats and thin film crystal mounts; software for efficient, user-friendly data acquisition, reduction and storage of both static and time-resolved data; and, novel experimental techniques. Collaborative and service projects will encompass X-ray diffraction from single crystals and from less-ordered membranes, fibers and solutions. Particular emphasis will be placed on crystals with very large unit cells such as viruses, and on time-resolved studies on time scales down to and including the x-ray pulse length at the APS, around 100 ps. These studies exploit the unique properties of the APS: its brilliance, intensity, and dedicated availability. Prior to the start-up of the APS in 1995-96, initial experiments and tests of X-ray equipment will be conducted at the National Synchrotron Light Source, on beam line X26C. BioCARS forms an integral part of the interdisciplinary Consortium for Advanced Radiation Sources, CARS. CARS consist of three universities (The University of Chicago, Northern Illinois University, and Southern Illinois University) and four national scientific groups, in structural biology (BioCARS), the earth and planetary sciences (GeoCARS), chemical sciences (ChemCARS) and soil and environmental science (Soil/EnviroCARS). CARS at present consists of 97 principal investigators, and is a Collaborative Access Team (CAT) which proposes to develop and operate two sectors at the APS, of which this request is for support of one. Substantial facilities, obtained with State and University support, are already in place or committed, including design, construction, X-ray, and metrology laboratories.

我们希望在高级设计，构建和操作一个部门 Argonne国家实验室正在建设的光子来源（AP）。 该行业将由一个弯曲器和Wiggler来源组成 磁铁源，相关的X射线光学元件，光束传输，三个 Hutches，BL3 Biohazards设施和实验设备合适 对于五种不同样式的结构生物学实验：病毒 晶体学，小角度散射，蛋白质 晶体学/多个波长异常分散，时间分辨 衍射和微晶的衍射。 该部门将是 为由29名成员组成的结构生物学家组成 生物剂（约占可用Beamtime的75％）和普通用户 （大约25％）。 技术研发将集中于：X射线 光学器件，包括传输光学和冷却方案 启用光学元件可以承受由 插入设备源，而没有大量退化 特性;储存磷和其他检测器；样品处理 液体氦低温器和薄膜晶体架等设备； 用于高效，用户友好的数据获取，减少和 静态和时间分辨数据的存储；而且，新颖的实验 技术。 协作和服务项目将包括X射线衍射 单晶体以及有序的膜，纤维和溶液。 特别重点将放在具有非常大单元的晶体上 诸如病毒之类的细胞，以及按时间分辨研究缩小的时间分辨研究 到AP处的X射线脉冲长度，约为100 ps。 这些研究利用了AP的独特特性：它的才华， 强度和专用的可用性。 在AP启动之前 在1995 - 96年，X射线设备的初始实验和测试将是 在Beam Line X26C上的国家同步器光源进行。 生物群是跨学科财团不可或缺的一部分 高级辐射源，汽车。汽车由三所大学组成 （芝加哥大学，北伊利诺伊大学和南部 伊利诺伊大学）和四个国家科学团体 生物学（生物群体），地球和行星科学（Geocars），化学 科学（Chemcars）以及土壤与环境科学 （土壤/环境）。 目前，汽车由97个校长组成 调查人员，是一个合作访问团队（CAT） 要在APS开发和运营两个扇区，此请求是 为了支持一个。 通过州和 大学的支持已经到位或承诺，包括设计， 建筑，X射线和计量实验室。